UniProtKB - Q5DU57 (SPT13_MOUSE)
Spermatogenesis-associated protein 13
Spata13
Functioni
Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA (By similarity).
Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
By similarity1 PublicationActivity regulationi
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: UniProtKB
- identical protein binding Source: MGI
GO - Biological processi
- cell migration Source: UniProtKB
- filopodium assembly Source: UniProtKB
- intracellular signal transduction Source: InterPro
- lamellipodium assembly Source: UniProtKB
- regulation of cell migration Source: UniProtKB
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Enzyme and pathway databases
Reactomei | R-MMU-9013148, CDC42 GTPase cycle R-MMU-9013149, RAC1 GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Spermatogenesis-associated protein 13Alternative name(s): APC-stimulated guanine nucleotide exchange factor 2 Short name: Asef2 |
Gene namesi | Name:Spata13 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104838, Spata13 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- ruffle membrane By similarity
Other locations
- filopodium By similarity
- lamellipodium By similarity
Note: Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Plasma Membrane
- ruffle membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- filopodium Source: UniProtKB
- lamellipodium Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000278449 | 1 – 656 | Spermatogenesis-associated protein 13Add BLAST | 656 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 82 | PhosphoserineCombined sources | 1 | |
Modified residuei | 118 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q5DU57 |
MaxQBi | Q5DU57 |
PaxDbi | Q5DU57 |
PeptideAtlasi | Q5DU57 |
PRIDEi | Q5DU57 |
ProteomicsDBi | 254542 [Q5DU57-1] 254543 [Q5DU57-2] |
PTM databases
iPTMneti | Q5DU57 |
PhosphoSitePlusi | Q5DU57 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts (via ABR and SH3 domain) with APC. The binding of APC enhances its GEF activity by relieving it from an autoinhibitory conformation, in which the ABR and SH3 domains are associated with the C-terminal tail.
Interacts (via C-terminal tail) with PPP1R9B (via C-terminus).
Interacts with RAC1 (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 230117, 2 interactors |
IntActi | Q5DU57, 2 interactors |
STRINGi | 10090.ENSMUSP00000022566 |
Miscellaneous databases
RNActi | Q5DU57, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 151 – 210 | SH3PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 244 – 428 | DHPROSITE-ProRule annotationAdd BLAST | 185 | |
Domaini | 459 – 565 | PHPROSITE-ProRule annotationAdd BLAST | 107 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 26 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 85 – 115 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 102 – 154 | ABR (APC-binding region) domainBy similarityAdd BLAST | 53 | |
Regioni | 215 – 242 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 565 – 656 | C-terminal tailBy similarityAdd BLAST | 92 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 16 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 218 – 241 | Basic and acidic residuesSequence analysisAdd BLAST | 24 |
Domaini
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG3519, Eukaryota |
InParanoidi | Q5DU57 |
OrthoDBi | 507598at2759 |
PhylomeDBi | Q5DU57 |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR001331, GDS_CDC24_CS IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS00741, DH_1, 1 hit PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHPASVTTTS QDPCAPSGSC RGGRRRRPIS VIGGVSFYGN TQVEDVENLL
60 70 80 90 100
VQPAARPPVP AHQVPPYKAV SARLRPFTFS QSTPIGLDRV GRRRQMKTSN
110 120 130 140 150
VSSDGGAESS ALVDDNGSEE DFSYEELCQA NPRYLQPGGE QLAINELISD
160 170 180 190 200
GSVVCAEALW DHVTMDDQEL GFKAGDVIQV LEASNKDWWW GRNEDKEAWF
210 220 230 240 250
PASFVRLRVN QEELPENCSS SHGEEQDEDT SKARHKHPES QQQMRTNVIQ
260 270 280 290 300
EIMNTERVYI KHLKDICEGY IRQCRKHTGM FTVAQLATIF GNIEDIYKFQ
310 320 330 340 350
RKFLKDLEKQ YNKEEPHLSE IGSCFLEHQE GFAIYSEYCN NHPGACVELS
360 370 380 390 400
NLMKHSKYRH FFEACRLLQQ MIDIALDGFL LTPVQKICKY PLQLAELLKY
410 420 430 440 450
TTQEHGDYNN IKAAYEAMKN VACLINERKR KLESIDKIAR WQVSIVGWEG
460 470 480 490 500
LDILDRSSEL IHSGELTKIT RQGKSQQRIF FLFDHQLVSC KKDLLRRDML
510 520 530 540 550
YYKGRMDMDE VELVDVEDGR DKDWSLSLRN AFKLVSKATD EVHLFCARKQ
560 570 580 590 600
EDKARWLQAY ADERRRVQED QQMGMEIPEN QKKLAMLNAQ KAGHGKSKGY
610 620 630 640 650
NSCPVAPPHQ SLPPLHQRHI TVPTSIPQQQ VFALAEPKRK PSIFWHTFHK
LTPFRK
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE0CYU0 | E0CYU0_MOUSE | Spermatogenesis-associated protein ... | Spata13 | 574 | Annotation score: | ||
E9Q3I3 | E9Q3I3_MOUSE | Spermatogenesis-associated protein ... | Spata13 | 1,244 | Annotation score: | ||
F6SRZ4 | F6SRZ4_MOUSE | Spermatogenesis-associated protein ... | Spata13 | 369 | Annotation score: | ||
F6V9S1 | F6V9S1_MOUSE | Spermatogenesis-associated protein ... | Spata13 | 240 | Annotation score: | ||
E0CXN6 | E0CXN6_MOUSE | Spermatogenesis-associated protein ... | Spata13 | 124 | Annotation score: | ||
E0CZ51 | E0CZ51_MOUSE | Spermatogenesis-associated protein ... | Spata13 | 138 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023287 | 1 – 352 | Missing in isoform 2. 1 PublicationAdd BLAST | 352 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK158193 mRNA Translation: BAE34401.1 AC151835 Genomic DNA No translation available. AC114000 Genomic DNA No translation available. AK220313 mRNA Translation: BAD90389.1 |
RefSeqi | NP_001028444.1, NM_001033272.2 NP_001297523.1, NM_001310594.1 |
Genome annotation databases
GeneIDi | 219140 |
KEGGi | mmu:219140 |
UCSCi | uc007ufe.2, mouse [Q5DU57-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK158193 mRNA Translation: BAE34401.1 AC151835 Genomic DNA No translation available. AC114000 Genomic DNA No translation available. AK220313 mRNA Translation: BAD90389.1 |
RefSeqi | NP_001028444.1, NM_001033272.2 NP_001297523.1, NM_001310594.1 |
3D structure databases
SMRi | Q5DU57 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230117, 2 interactors |
IntActi | Q5DU57, 2 interactors |
STRINGi | 10090.ENSMUSP00000022566 |
PTM databases
iPTMneti | Q5DU57 |
PhosphoSitePlusi | Q5DU57 |
Proteomic databases
EPDi | Q5DU57 |
MaxQBi | Q5DU57 |
PaxDbi | Q5DU57 |
PeptideAtlasi | Q5DU57 |
PRIDEi | Q5DU57 |
ProteomicsDBi | 254542 [Q5DU57-1] 254543 [Q5DU57-2] |
Genome annotation databases
GeneIDi | 219140 |
KEGGi | mmu:219140 |
UCSCi | uc007ufe.2, mouse [Q5DU57-1] |
Organism-specific databases
CTDi | 221178 |
MGIi | MGI:104838, Spata13 |
Phylogenomic databases
eggNOGi | KOG3519, Eukaryota |
InParanoidi | Q5DU57 |
OrthoDBi | 507598at2759 |
PhylomeDBi | Q5DU57 |
Enzyme and pathway databases
Reactomei | R-MMU-9013148, CDC42 GTPase cycle R-MMU-9013149, RAC1 GTPase cycle |
Miscellaneous databases
BioGRID-ORCSi | 219140, 0 hits in 63 CRISPR screens |
ChiTaRSi | Spata13, mouse |
PROi | PR:Q5DU57 |
RNActi | Q5DU57, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR001331, GDS_CDC24_CS IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS00741, DH_1, 1 hit PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SPT13_MOUSE | |
Accessioni | Q5DU57Primary (citable) accession number: Q5DU57 Secondary accession number(s): Q3TZ09 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | February 20, 2007 | |
Last modified: | February 23, 2022 | |
This is version 119 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot