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Protein

Ubiquitin carboxyl-terminal hydrolase 22

Gene

Usp22

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185NucleophilePROSITE-ProRule annotation1
Active sitei479Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri61 – 121UBP-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Chromatin regulator, Hydrolase, Protease, Thiol protease
Biological processCell cycle, Transcription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiC19.075

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 22 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22
Gene namesi
Name:Usp22
Synonyms:Kiaa1063
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2144157 Usp22

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806511 – 525Ubiquitin carboxyl-terminal hydrolase 22Add BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei129N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ5DU02
PaxDbiQ5DU02
PeptideAtlasiQ5DU02
PRIDEiQ5DU02

PTM databases

iPTMnetiQ5DU02
PhosphoSitePlusiQ5DU02

Expressioni

Tissue specificityi

Highly expressed in brain and weakly in other organs.1 Publication

Developmental stagei

Highly expressed between E10.5 and E12.5. Expressed in the midbrain, forebrain, hindbrain and dorsal root ganglia of embryos at E12.5.1 Publication

Gene expression databases

BgeeiENSMUSG00000042506 Expressed in 283 organ(s), highest expression level in brain
CleanExiMM_USP22
ExpressionAtlasiQ5DU02 baseline and differential
GenevisibleiQ5DU02 MM

Interactioni

Subunit structurei

Component of some SAGA transcription coactivator-HAT complexes, at least composed of ATXN7, ATXN7L3, ENY2, GCN5L2, SUPT3H, TAF10, TRRAP and USP22. Within the SAGA complex, ATXN7L3, ENY2 and USP22 form a subcomplex required for histone deubiquitination. Interacts directly with ATXN7L3; leading to its recruitment to the SAGA complex. Interacts with ATXN7L3 and weakly with ATXN7L3B.By similarity

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-916 TFTC histone acetylation complex
CPX-920 SAGA complex
IntActiQ5DU02, 2 interactors
MINTiQ5DU02
STRINGi10090.ENSMUSP00000041263

Structurei

3D structure databases

ProteinModelPortaliQ5DU02
SMRiQ5DU02
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini176 – 520USPAdd BLAST345

Sequence similaritiesi

Belongs to the peptidase C19 family. UBP8 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri61 – 121UBP-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1867 Eukaryota
ENOG410XQQ0 LUCA
GeneTreeiENSGT00890000139343
HOVERGENiHBG058014
InParanoidiQ5DU02
KOiK11366
OMAiCDDHLIT
OrthoDBiEOG091G09GI
PhylomeDBiQ5DU02
TreeFamiTF323554

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
IPR013083 Znf_RING/FYVE/PHD
IPR001607 Znf_UBP
PfamiView protein in Pfam
PF00443 UCH, 1 hit
PF02148 zf-UBP, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit
PS50271 ZF_UBP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q5DU02-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVARPEPEVE AMDAELAVPP PGCSHLGSFK VDNWKQNLRA IYQCFVWSGT
60 70 80 90 100
AEARKRKAKS CVCHVCGIHL NRLHSCLYCV FFGCFTKKHI HDHAKSKRHN
110 120 130 140 150
LAIDLMYGGI YCFLCQDYIY DKDIEIIAKE EQRKAWKMQG VGEKFSTWEP
160 170 180 190 200
TKRELELLKH NPKRRKITSN CTIGLRGLIN LGNTCFMNCI VQALTHTPLL
210 220 230 240 250
RDFFLSDRHR CEMQSPSSCL VCEMSSLFQE FYSGHRSPHI PYKLLHLVWT
260 270 280 290 300
HARHLAGYEQ QDAHEFLIAA LDVLHRHCKG DDNGKKANNP NHCNCIIDQI
310 320 330 340 350
FTGGLQSDVT CQVCHGVSTT IDPFWDISLD LPGSSTPFWP LSPGSEGSVV
360 370 380 390 400
NGESHASGTT TLTDCLRRFT RPEHLGSSAK IKCSGCHSYQ ESTKQLTMKK
410 420 430 440 450
LPIVACFHLK RFEHSAKLRR KITTYVSFPL ELDMTPFMAS SKESRMNGQY
460 470 480 490 500
QQPLDSLNND NKYSLFAVVN HQGTLESGHY TSFIRQHKDQ WFKCDDAIIT
510 520
KASIKDVLDS EGYLLFYHKQ FLEYE
Length:525
Mass (Da):59,954
Last modified:September 13, 2005 - v2
Checksum:iC4CD69A0317CD0EF
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BLQ1H3BLQ1_MOUSE
Ubiquitin carboxyl-terminal hydrola...
Usp22
21Annotation score:

Sequence cautioni

The sequence BAD90248 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77L → H in BAE36137 (PubMed:16141072).Curated1
Sequence conflicti188N → D in AAH80737 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK084022 mRNA Translation: BAC39100.1
AK220368 mRNA Translation: BAD90248.1 Different initiation.
AK155071 mRNA Translation: BAE33026.1
AK160992 mRNA Translation: BAE36137.1
AL646093 Genomic DNA No translation available.
BC058419 mRNA Translation: AAH58419.1
BC080737 mRNA Translation: AAH80737.1
CCDSiCCDS24807.1
RefSeqiNP_001004143.2, NM_001004143.4
UniGeneiMm.30602

Genome annotation databases

EnsembliENSMUST00000041683; ENSMUSP00000041263; ENSMUSG00000042506
GeneIDi216825
KEGGimmu:216825
UCSCiuc007jhc.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK084022 mRNA Translation: BAC39100.1
AK220368 mRNA Translation: BAD90248.1 Different initiation.
AK155071 mRNA Translation: BAE33026.1
AK160992 mRNA Translation: BAE36137.1
AL646093 Genomic DNA No translation available.
BC058419 mRNA Translation: AAH58419.1
BC080737 mRNA Translation: AAH80737.1
CCDSiCCDS24807.1
RefSeqiNP_001004143.2, NM_001004143.4
UniGeneiMm.30602

3D structure databases

ProteinModelPortaliQ5DU02
SMRiQ5DU02
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-916 TFTC histone acetylation complex
CPX-920 SAGA complex
IntActiQ5DU02, 2 interactors
MINTiQ5DU02
STRINGi10090.ENSMUSP00000041263

Protein family/group databases

MEROPSiC19.075

PTM databases

iPTMnetiQ5DU02
PhosphoSitePlusiQ5DU02

Proteomic databases

MaxQBiQ5DU02
PaxDbiQ5DU02
PeptideAtlasiQ5DU02
PRIDEiQ5DU02

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041683; ENSMUSP00000041263; ENSMUSG00000042506
GeneIDi216825
KEGGimmu:216825
UCSCiuc007jhc.2 mouse

Organism-specific databases

CTDi23326
MGIiMGI:2144157 Usp22
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1867 Eukaryota
ENOG410XQQ0 LUCA
GeneTreeiENSGT00890000139343
HOVERGENiHBG058014
InParanoidiQ5DU02
KOiK11366
OMAiCDDHLIT
OrthoDBiEOG091G09GI
PhylomeDBiQ5DU02
TreeFamiTF323554

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases

Miscellaneous databases

PROiPR:Q5DU02
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042506 Expressed in 283 organ(s), highest expression level in brain
CleanExiMM_USP22
ExpressionAtlasiQ5DU02 baseline and differential
GenevisibleiQ5DU02 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
IPR013083 Znf_RING/FYVE/PHD
IPR001607 Znf_UBP
PfamiView protein in Pfam
PF00443 UCH, 1 hit
PF02148 zf-UBP, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit
PS50271 ZF_UBP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBP22_MOUSE
AccessioniPrimary (citable) accession number: Q5DU02
Secondary accession number(s): Q3TU34
, Q3U2W4, Q5SU81, Q66JV8, Q6PDX3, Q8BJG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 7, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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