Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidylinositol 3-kinase catalytic subunit type 3

Gene

PIK3C3

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).By similarity

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

Cofactori

Mn2+By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAutophagy, Cell cycle, Cell division
LigandATP-binding, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase catalytic subunit type 3 (EC:2.7.1.137)
Short name:
PI3-kinase type 3
Short name:
PI3K type 3
Short name:
PtdIns-3-kinase type 3
Alternative name(s):
Phosphoinositide-3-kinase class 3
Gene namesi
Name:PIK3C3By similarity
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000888041 – 887Phosphatidylinositol 3-kinase catalytic subunit type 3Add BLAST887

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei163Phosphothreonine; by AMPKBy similarity1
Modified residuei165Phosphoserine; by AMPKBy similarity1
Modified residuei244PhosphoserineBy similarity1
Modified residuei261PhosphoserineBy similarity1
Modified residuei282PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5D891
PeptideAtlasiQ5D891
PRIDEiQ5D891

Interactioni

Subunit structurei

Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are: the PI3K complex I (PI3KC3-C1) containing ATG14, and the PI3K complex II (PI3KC3-C2) containing UVRAG. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits such as RUBCN, SH3GLB1/Bif-1 and AMBRA1. PI3KC3-C1 probably associates with PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Interacts with AMBRA1. Interacts with BECN1P1/BECN2. Interacts with SLAMF1. May be a component of a complex composed of RAB5A (in GDP-bound form), DYN2 and PIK3C3 (By similarity). Interacts with NCKAP1L (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000004057

Structurei

3D structure databases

ProteinModelPortaliQ5D891
SMRiQ5D891
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 184C2 PI3K-typePROSITE-ProRule annotationAdd BLAST150
Domaini282 – 520PIK helicalPROSITE-ProRule annotationAdd BLAST239
Domaini631 – 885PI3K/PI4KPROSITE-ProRule annotationAdd BLAST255

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0906 Eukaryota
COG5032 LUCA
HOGENOMiHOG000174003
HOVERGENiHBG082145
InParanoidiQ5D891
KOiK00914

Family and domain databases

Gene3Di1.10.1070.11, 1 hit
2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR035892 C2_domain_sf
IPR011009 Kinase-like_dom_sf
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR002420 PI3K_C2_dom
IPR008290 PI3K_Vps34
IPR015433 PI_Kinase
IPR001263 PInositide-3_kin_accessory_dom
PANTHERiPTHR10048 PTHR10048, 1 hit
PfamiView protein in Pfam
PF00454 PI3_PI4_kinase, 1 hit
PF00792 PI3K_C2, 1 hit
PF00613 PI3Ka, 1 hit
PIRSFiPIRSF000587 PI3K_Vps34, 1 hit
SMARTiView protein in SMART
SM00142 PI3K_C2, 1 hit
SM00145 PI3Ka, 1 hit
SM00146 PI3Kc, 1 hit
SUPFAMiSSF48371 SSF48371, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS51547 PI3K_C2, 1 hit
PS51545 PIK_HELICAL, 1 hit

Sequencei

Sequence statusi: Complete.

Q5D891-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGEAEKFHYI YSCDLDINVQ LKIGSLEGKR EQKSYKAVLE DPMLKFSGLY
60 70 80 90 100
QETCSDLYVT CQVFAEGKPL ALPVRTSYKA FSTRWNWNEW LKLPVKYPDL
110 120 130 140 150
PRNAQVALTI WDVYGPGKAV PVGGTTVSLF GKYGMFRQGM HDLKVWPNVE
160 170 180 190 200
ADGSEPTKTP GRTSSTLSED QMSRLAKLTK AHRQGHMVKV DWLDRLTFRE
210 220 230 240 250
IEMINESEKR SSNFMYLMVE FRCVKCDDKE YGIVYYEKDG DESSPILTGF
260 270 280 290 300
EIVKVPDPQM SMENLVESKH HKLARSLRSG PSDHGLKPNA ATRDQLNIIV
310 320 330 340 350
SYPPTKQLTY EEQDLVWKFR YYLTNQEKAL TKFLKCVNWD LPQEAKQALE
360 370 380 390 400
LLGKWKPMDV EDSLELLSSH YANPTVRRYA VARLRQADDE DLLMYLLQLV
410 420 430 440 450
QALKYENFDD IKNGLEPTKK ESQGSVSESV SNSGIGSAEI DSSQIITSPL
460 470 480 490 500
PPVSSPPPAS KTKESSDGES LEQDLCTFLI SRACKNSTLA NYLYWYVIVE
510 520 530 540 550
CEGQDTQQRD PKTHEMYLNV MRRFSQALLK GDKSVRVMRS LLAAQQTFVD
560 570 580 590 600
RLVHLMKAVQ RESGNRKKKN ERLQALLGDN EKMNLSDVEL IPLPLEPQVK
610 620 630 640 650
IRGIIPETAT LFKSALMPAQ LFFKTEDGGK YPVIFKHGDD LRQDQLILQI
660 670 680 690 700
ISLMDKLLRK ENLDLKLTPY KVLATSTKHG FMQFIQSVPV AEVLDTEGSI
710 720 730 740 750
QNFFRKYAPS ENGPNGISAE VMDTYVKSCA GYCVITYILG VGDRHLDNLL
760 770 780 790 800
LTKTGKLFHI DFGYILGRDP KPLPPPMKLN KEMVEGMGGT QSEQYQEFRK
810 820 830 840 850
QCYTAFLHLR RYSNLILNLF SLMVDANIPD IALEPDKTVK KVQDKFRLDL
860 870 880
SDEEAVHYMQ SLIDESVHAL FAAVVEQIHK FAQYWRK
Length:887
Mass (Da):101,234
Last modified:March 29, 2005 - v1
Checksum:i1D1D954B3A6BB433
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY823302 mRNA Translation: AAX12416.1
RefSeqiNP_001012974.1, NM_001012956.2
UniGeneiSsc.13366

Genome annotation databases

GeneIDi503700
KEGGissc:503700

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY823302 mRNA Translation: AAX12416.1
RefSeqiNP_001012974.1, NM_001012956.2
UniGeneiSsc.13366

3D structure databases

ProteinModelPortaliQ5D891
SMRiQ5D891
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000004057

Proteomic databases

PaxDbiQ5D891
PeptideAtlasiQ5D891
PRIDEiQ5D891

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi503700
KEGGissc:503700

Organism-specific databases

CTDi5289

Phylogenomic databases

eggNOGiKOG0906 Eukaryota
COG5032 LUCA
HOGENOMiHOG000174003
HOVERGENiHBG082145
InParanoidiQ5D891
KOiK00914

Family and domain databases

Gene3Di1.10.1070.11, 1 hit
2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR035892 C2_domain_sf
IPR011009 Kinase-like_dom_sf
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR002420 PI3K_C2_dom
IPR008290 PI3K_Vps34
IPR015433 PI_Kinase
IPR001263 PInositide-3_kin_accessory_dom
PANTHERiPTHR10048 PTHR10048, 1 hit
PfamiView protein in Pfam
PF00454 PI3_PI4_kinase, 1 hit
PF00792 PI3K_C2, 1 hit
PF00613 PI3Ka, 1 hit
PIRSFiPIRSF000587 PI3K_Vps34, 1 hit
SMARTiView protein in SMART
SM00142 PI3K_C2, 1 hit
SM00145 PI3Ka, 1 hit
SM00146 PI3Kc, 1 hit
SUPFAMiSSF48371 SSF48371, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS51547 PI3K_C2, 1 hit
PS51545 PIK_HELICAL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPK3C3_PIG
AccessioniPrimary (citable) accession number: Q5D891
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: March 29, 2005
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again