UniProtKB - Q5CD77 (TBC14_RAT)
Protein
TBC1 domain family member 14
Gene
Tbc1d14
Organism
Rattus norvegicus (Rat)
Status
Functioni
Plays a role in the regulation of starvation-induced autophagosome formation. Together with the TRAPPIII complex, regulates a constitutive trafficking step from peripheral recycling endosomes to the early Golgi, maintaining the cycling pool of ATG9 required for initiation of autophagy.By similarity
GO - Molecular functioni
- GTPase activator activity Source: GO_Central
- protein kinase binding Source: RGD
- Rab GTPase binding Source: GO_Central
GO - Biological processi
- activation of GTPase activity Source: GO_Central
- intracellular protein transport Source: GO_Central
- negative regulation of autophagy Source: RGD
- recycling endosome to Golgi transport Source: RGD
- regulation of autophagosome assembly Source: RGD
Keywordsi
Molecular function | GTPase activation |
Enzyme and pathway databases
Reactomei | R-RNO-8854214, TBC/RABGAPs |
Names & Taxonomyi
Protein namesi | Recommended name: TBC1 domain family member 14Alternative name(s): Spermatogenesis-related factor 2 Short name: SRF-2 Up-regulated in nephrectomized rat kidney #2 |
Gene namesi | Name:Tbc1d14 ORF Names:UR-NR#2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1309993, Tbc1d14 |
Subcellular locationi
Golgi apparatus
- cis-Golgi network By similarity
- trans-Golgi network By similarity
Note: After amino acid starvation, Golgi apparatus-associated protein levels increase compared with fed conditions. May be cycling between the Golgi apparatus and an endosomal pool, redistributing to the Golgi apparatus upon starvation.By similarity
Cytosol
- cytosol Source: GOC
Endosome
- recycling endosome Source: RGD
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Other locations
- autophagosome Source: RGD
Keywords - Cellular componenti
Golgi apparatusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000319418 | 1 – 694 | TBC1 domain family member 14Add BLAST | 694 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 92 | PhosphoserineBy similarity | 1 | |
Modified residuei | 296 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q5CD77 |
PTM databases
PhosphoSitePlusi | Q5CD77 |
Expressioni
Tissue specificityi
PubMed:15758561 detected expression at the stage of sexual maturation in testis, mainly in the spermatocytes. No expression detected in the ovary, brain, heart, lung, liver and kidney. PubMed:15200410 detected expression in brain, heart, lung, liver, spleen and kidney but not in small intestine.2 Publications
Developmental stagei
First detected in the testis at 5 weeks. Level of expression increases up to 7 weeks and maintains even at 63 weeks.1 Publication
Inductioni
Up-regulated in nephrectomized kidney.1 Publication
Interactioni
Subunit structurei
Interacts with ULK1. May interact with RAB11A and RAB11B, but does not exhibit any GTPase-activating activity toward these proteins.
Interacts with TRAPPC8.
By similarityGO - Molecular functioni
- protein kinase binding Source: RGD
- Rab GTPase binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000055215 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 402 – 612 | Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST | 211 |
Phylogenomic databases
eggNOGi | KOG2223, Eukaryota |
InParanoidi | Q5CD77 |
OrthoDBi | 798837at2759 |
PhylomeDBi | Q5CD77 |
Family and domain databases
InterProi | View protein in InterPro IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf |
Pfami | View protein in Pfam PF00566, RabGAP-TBC, 1 hit |
SMARTi | View protein in SMART SM00164, TBC, 1 hit |
SUPFAMi | SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50086, TBC_RABGAP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q5CD77-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDGNLSTSM NGVALMGILD GRPGDSLQEL QHLSIKAVPR SLSVPDYGPT
60 70 80 90 100
LKLGALEDRH SLQSVDSGIP TLEIGNPEPV PCSVVHVKRK QSESEIIPER
110 120 130 140 150
AFQSACPLPS CTPSAPTGSE REQAVRKSST FPRTGYDSVK LYSPTSKALS
160 170 180 190 200
RSDDVSVCSV SSLGTELSTT LSVSNEDILD LMVTSNSSAI VTLENDDDPQ
210 220 230 240 250
FTDVTLSSIK ETSDLHQQDC VAETEEGRKL RLLQPFSHFF TRNLLARKQN
260 270 280 290 300
ARLDRQRDLG WKLFGKVPLR ETAQKDSKKT QKEYEDKAGR PSRPPSPKQN
310 320 330 340 350
VRKNLDFEPL STTALILEDR PANLPAKPAE EAQKHRQQYE EMVVQAKKRE
360 370 380 390 400
LKEAQRRKKQ LEERCKVEES IGNAVLTWNN EILPNWETMW CSKKVRDLWW
410 420 430 440 450
QGIPPSVRGK VWSLAIGNEL NITHELFDIC LARAKERWRS LSTGGSEVEN
460 470 480 490 500
EDAGFSAADR EASLELIKLD ISRTFPNLCI FQQGGPYHDM LHSILGAYTC
510 520 530 540 550
YRPDVGYVQG MSFIAAVLIL NLDTADAFIA FSNLLNKPCQ MAFFRVDHGL
560 570 580 590 600
MLTYFAAFEV FFEENLPKLF AHFKKNNLTA DIYLIDWIFT LYSKSLPLDL
610 620 630 640 650
ACRIWDVFCR DGEEFLFRTA LGILKLFEDI LTRMDFIHSA QFLTRLPEDL
660 670 680 690
PADDVFAAIS TVQMQSRNKK WAQVLSALQK DSREMEEGSP SVRD
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2KAD0 | A0A0G2KAD0_RAT | TBC1 domain family member 14 | Tbc1d14 | 722 | Annotation score: | ||
A0A0G2K7D5 | A0A0G2K7D5_RAT | TBC1 domain family member 14 | Tbc1d14 | 694 | Annotation score: | ||
G3V9P9 | G3V9P9_RAT | TBC1 domain family member 14 | Tbc1d14 rCG_36199 | 714 | Annotation score: | ||
A0A0G2K2K3 | A0A0G2K2K3_RAT | TBC1 domain family member 14 | Tbc1d14 | 413 | Annotation score: |
Sequence cautioni
The sequence AAH99760 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD23893 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD91010 differs from that shown. Reason: Frameshift.Curated
The sequence BAD91010 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 278 – 282 | KKTQK → GLASM in BAD23893 (PubMed:15200410).Curated | 5 | |
Sequence conflicti | 304 | N → S in BAD23893 (PubMed:15200410).Curated | 1 | |
Sequence conflicti | 416 | I → M in BAD23893 (PubMed:15200410).Curated | 1 | |
Sequence conflicti | 420 | L → V in BAD23893 (PubMed:15200410).Curated | 1 | |
Sequence conflicti | 431 | L → F in BAD23893 (PubMed:15200410).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB117752 mRNA Translation: BAD91010.1 Sequence problems. BC099760 mRNA Translation: AAH99760.1 Different initiation. AB108668 mRNA Translation: BAD23893.1 Different initiation. |
RefSeqi | NP_001012152.2, NM_001012152.2 NP_001029193.2, NM_001034021.2 NP_001106836.1, NM_001113365.1 |
Genome annotation databases
GeneIDi | 360956 |
KEGGi | rno:360956 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB117752 mRNA Translation: BAD91010.1 Sequence problems. BC099760 mRNA Translation: AAH99760.1 Different initiation. AB108668 mRNA Translation: BAD23893.1 Different initiation. |
RefSeqi | NP_001012152.2, NM_001012152.2 NP_001029193.2, NM_001034021.2 NP_001106836.1, NM_001113365.1 |
3D structure databases
SMRi | Q5CD77 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000055215 |
PTM databases
PhosphoSitePlusi | Q5CD77 |
Proteomic databases
PaxDbi | Q5CD77 |
Genome annotation databases
GeneIDi | 360956 |
KEGGi | rno:360956 |
Organism-specific databases
CTDi | 57533 |
RGDi | 1309993, Tbc1d14 |
Phylogenomic databases
eggNOGi | KOG2223, Eukaryota |
InParanoidi | Q5CD77 |
OrthoDBi | 798837at2759 |
PhylomeDBi | Q5CD77 |
Enzyme and pathway databases
Reactomei | R-RNO-8854214, TBC/RABGAPs |
Miscellaneous databases
PROi | PR:Q5CD77 |
Family and domain databases
InterProi | View protein in InterPro IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf |
Pfami | View protein in Pfam PF00566, RabGAP-TBC, 1 hit |
SMARTi | View protein in SMART SM00164, TBC, 1 hit |
SUPFAMi | SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50086, TBC_RABGAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TBC14_RAT | |
Accessioni | Q5CD77Primary (citable) accession number: Q5CD77 Secondary accession number(s): Q499U3, Q6I7R4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | February 26, 2008 | |
Last modified: | December 2, 2020 | |
This is version 80 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |