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UniProtKB - Q5C9I9 (ISPD_MENPI)
Protein
(-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrial
Gene
N/A
Organism
Mentha piperita (Peppermint) (Mentha aquatica x Mentha spicata)
Status
Functioni
Involved in the biosynthesis of menthol and related monoterpenes in leaves. Can use (-)-trans-carveol and, with a lower relative velocity, (-)-trans-isopiperitenol, (+)-neomenthol, (+)-neoisomenthol and (-)-cis-isopiperitenol as substrates, but not (-)-cis-carvenol, (-)-menthol, (+)-isomenthol, 7-hydroxy-limonene, (-)-isopiperitenone or (-)-carvone.
Miscellaneous
Can use both NAD+ or NADP+ as cofactor.
Catalytic activityi
Kineticsi
kcat is 0.02 sec(-1) with (-)-trans-carveol as substrate (at pH 7.5), kcat is 0.06 sec(-1) with (-)-trans-carveol as substrate (at pH 10), kcat is 0.002 sec(-1) with (-)-trans-isopiperitenol as substrate (at pH 7.5).
- KM=1.8 µM for (-)-trans-carveol (at pH 7.5)1 Publication
- KM=72 µM for (-)-trans-isopiperitenol (at pH 7.5)1 Publication
- KM=410 µM for NAD+ (at pH 7.5)1 Publication
pH dependencei
Optimum pH is 10.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 147 | SubstrateBy similarity | 1 | |
Active sitei | 160 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 13 – 38 | NADBy similarityAdd BLAST | 26 |
GO - Molecular functioni
- carveol dehydrogenase activity Source: UniProtKB
- isopiperitenol dehydrogenase activity Source: UniProtKB
- nucleotide binding Source: UniProtKB
GO - Biological processi
- menthol biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD, NADP |
Names & Taxonomyi
Protein namesi | |
Organismi | Mentha piperita (Peppermint) (Mentha aquatica x Mentha spicata) |
Taxonomic identifieri | 34256 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Lamiales › Lamiaceae › Nepetoideae › Mentheae › Menthinae › Mentha |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 30 | MitochondrionSequence analysisAdd BLAST | 30 | |
ChainiPRO_0000418415 | 31 – 265 | (-)-isopiperitenol/(-)-carveol dehydrogenase, mitochondrialAdd BLAST | 235 |
Expressioni
Tissue specificityi
Peltate glandular trichomes.
Interactioni
Subunit structurei
Homodimer and homotetramer.
1 PublicationFamily & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Keywords - Domaini
Transit peptideFamily and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH |
SUPFAMi | SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q5C9I9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASVKKLAGK VAIVTGGASG IGEVTARLFA ERGARAVVIA DMQPEKGGTV
60 70 80 90 100
AESIGGRRCS YVHCDITDEQ QVRSVVDWTA ATYGGVDVMF CNAGTASATA
110 120 130 140 150
QTVLDLDLAQ FDRVMRVNAR GTAACVKQAA RKMVELGRGG AIICTASATV
160 170 180 190 200
HHAGPNLTDY IMSKCGVLGL VRSASLQLGV HGIRVNSVSP TALATPLTAT
210 220 230 240 250
IGLRTAADVE SFYGQVTSLK GVAITAEHVA EAVAFLASDE AAFVTGHDLA
260
VDGGLQCLPF VAVAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY641428 mRNA Translation: AAU20370.1 |
Genome annotation databases
KEGGi | ag:AAU20370 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY641428 mRNA Translation: AAU20370.1 |
3D structure databases
AlphaFoldDBi | Q5C9I9 |
SMRi | Q5C9I9 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:AAU20370 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ISPD_MENPI | |
Accessioni | Q5C9I9Primary (citable) accession number: Q5C9I9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2013 |
Last sequence update: | April 12, 2005 | |
Last modified: | May 25, 2022 | |
This is version 66 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |