UniProtKB - Q5BL50 (AT8B1_XENTR)
Protein
Phospholipid-transporting ATPase IC
Gene
atp8b1
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Functioni
Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids (By similarity). Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. May also participate in the establishment of the canalicular membrane integrity by ensuring asymmetric distribution of phospholipids in the canicular membrane (By similarity).By similarity
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).By similarity EC:7.6.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 454 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 891 | MagnesiumBy similarity | 1 | |
Metal bindingi | 895 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase-coupled intramembrane lipid transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- magnesium ion binding Source: InterPro
- phosphatidylserine flippase activity Source: UniProtKB
GO - Biological processi
- apical protein localization Source: UniProtKB
- Golgi organization Source: GO_Central
- phospholipid translocation Source: GO_Central
- regulation of chloride transport Source: UniProtKB
Keywordsi
Molecular function | Translocase |
Biological process | Lipid transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-XTR-936837, Ion transport by P-type ATPases |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipid-transporting ATPase IC (EC:7.6.2.1)Alternative name(s): ATPase class I type 8B member 1 P4-ATPase flippase complex alpha subunit atp8b1 |
Gene namesi | Name:atp8b1 |
Organismi | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
Taxonomic identifieri | 8364 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Proteomesi |
|
Organism-specific databases
Xenbasei | XB-GENE-490143, atp8b1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
- Apical cell membrane By similarity
Golgi apparatus
- Golgi apparatus By similarity
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Other locations
- stereocilium By similarity
Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A or TMEM30B. Localizes to apical membranes in epithelial cells.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Golgi apparatus
- trans-Golgi network Source: GO_Central
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- phospholipid-translocating ATPase complex Source: UniProtKB
- stereocilium Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 133 | CytoplasmicSequence analysisAdd BLAST | 133 | |
Transmembranei | 134 – 154 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 155 – 338 | Exoplasmic loopSequence analysisAdd BLAST | 184 | |
Transmembranei | 339 – 359 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 360 – 385 | CytoplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 386 – 406 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 407 – 956 | Exoplasmic loopSequence analysisAdd BLAST | 550 | |
Transmembranei | 957 – 977 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 978 – 980 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 981 – 1001 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1002 – 1034 | Exoplasmic loopSequence analysisAdd BLAST | 33 | |
Transmembranei | 1035 – 1055 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1056 – 1069 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 1070 – 1090 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1091 – 1092 | Exoplasmic loopSequence analysis | 2 | |
Transmembranei | 1093 – 1113 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1114 – 1117 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 1118 – 1138 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1139 – 1140 | Exoplasmic loopSequence analysis | 2 | |
Transmembranei | 1141 – 1161 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1162 – 1250 | CytoplasmicSequence analysisAdd BLAST | 89 |
Keywords - Cellular componenti
Cell membrane, Cell projection, Endoplasmic reticulum, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000370863 | 1 – 1250 | Phospholipid-transporting ATPase ICAdd BLAST | 1250 |
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit. The flippase ATP8B1:TMEM30A complex can form an intermediate phosphoenzyme in vitro.
Also interacts with beta subunit TMEM30B.
By similarityProtein-protein interaction databases
STRINGi | 8364.ENSXETP00000045089 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 478 – 481 | Poly-Asp | 4 |
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0206, Eukaryota |
InParanoidi | Q5BL50 |
OMAi | TSIYERW |
OrthoDBi | 587717at2759 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit |
InterProi | View protein in InterPro IPR030346, ATP8B1 IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
PANTHERi | PTHR24092, PTHR24092, 1 hit PTHR24092:SF48, PTHR24092:SF48, 1 hit |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5BL50-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDTDYESTYE DDSQVPNDDV VPYSDDETDD ELDSPQTDEP EQNRRNVQAE
60 70 80 90 100
QSREPVIKEC TWQVKANDRN FYDQPEFKKK VFLCLKKSKY AGNAIKTYKY
110 120 130 140 150
NPITFLPVNL YEQFKRAANA YFLVLLILQT IPQISTVTWS TTLIPLLLVL
160 170 180 190 200
GITAIKDLVD DIARHKMDNE INNRPSEVIT DGRFKKTKWK HIHVGDIIRI
210 220 230 240 250
NKNEFVPADV LLLSSSDPNS LCYVETAELD GETNLKFKMS LEITDKLLQK
260 270 280 290 300
EEQLAGFDGL VECEEPNNRL DKFVGTLFWR GNSFGLDADK ILLRGCTVRN
310 320 330 340 350
TEYCHGLVLF AGADTKIMRN SGKTRLKRTK IDYLMNYMVY TIFVLLILAA
360 370 380 390 400
AGLAIGQTFW EAKLGAANVS WYLYDGNNYS PSYRGFLAFW GYIIVLNTMV
410 420 430 440 450
PISLYVSVEV IRLGQSYFIN WDLQMYFSPK DTPAKARTTT LNEQLGQIQY
460 470 480 490 500
IFSDKTGTLT QNIMTFKKCT INGTTYGDDD DELKSGQTKQ VDFSWNPLAD
510 520 530 540 550
PSFTFHDNYL IEQIRAGKDK DVYEFFKLLA LCHTVMAEKT DGELIYQAAS
560 570 580 590 600
PDEGALVTAA RNFGFVFLSR TQSTITISEL GQEKTYEVLA ILDFNSDRKR
610 620 630 640 650
MSIIVRQPDG RIRLYCKGAD TVIYERLHPD NPIKDQTQKA LDIFANASLR
660 670 680 690 700
TLCLCYKDIN KGDFENWSKK YKQASVATSN RDEALDRVYE AIETDLKLLG
710 720 730 740 750
ATAIEDKLQD DVSGTIFNLA RADIKIWVLT GDKKETAENI GYSCKLLDDD
760 770 780 790 800
TEILYGEDIN VHLQTRMENQ RNQMSGNQGA QSNQSGAFLP TDKKHALIIT
810 820 830 840 850
GSWLNEILLE KKKRKKKRLK LKFPRTKEEK EQQLHEKLKA YALKEQRQRS
860 870 880 890 900
FVDLACECSA VICCRVTPKQ KAMVVDLVKR YKKAVTLAIG DGANDVNMIK
910 920 930 940 950
TAHIGVGISG QEGMQAVMSS DYSFAQFRYL QRLLLVHGRW SYIRMCKFLR
960 970 980 990 1000
YFFYKNFSFT LVHFWYSFFN GFSAQTVYED WFITLYNVLY SSLPVLLVGL
1010 1020 1030 1040 1050
LDQDVSDKLS LAFPRLYVPG QKDLLFNYKK FFLSLFHGIV TSLIIFFIPY
1060 1070 1080 1090 1100
GAFLLTMGQD GEAPSDYQSF AVTTATALVI TVNFQIGLDT SYWTFVNAFS
1110 1120 1130 1140 1150
IFGSIAIYFG IMFDLHSAGI HVLFPSMFIF TGAAPNALRQ PYLWLTIILT
1160 1170 1180 1190 1200
VAFCLLPIVA LRFLAKTIWP SESDKIQKKG KKFKAEVEQR AKPKPFARGV
1210 1220 1230 1240 1250
STRRSAYAFS HQRGYADLIS SGRSIRKKRA SLDAVFDNYP AQITHFTPQT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC090602 mRNA Translation: AAH90602.1 |
RefSeqi | NP_001025562.1, NM_001030391.1 |
Genome annotation databases
GeneIDi | 594952 |
KEGGi | xtr:594952 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC090602 mRNA Translation: AAH90602.1 |
RefSeqi | NP_001025562.1, NM_001030391.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 8364.ENSXETP00000045089 |
Genome annotation databases
GeneIDi | 594952 |
KEGGi | xtr:594952 |
Organism-specific databases
CTDi | 5205 |
Xenbasei | XB-GENE-490143, atp8b1 |
Phylogenomic databases
eggNOGi | KOG0206, Eukaryota |
InParanoidi | Q5BL50 |
OMAi | TSIYERW |
OrthoDBi | 587717at2759 |
Enzyme and pathway databases
Reactomei | R-XTR-936837, Ion transport by P-type ATPases |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit |
InterProi | View protein in InterPro IPR030346, ATP8B1 IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
PANTHERi | PTHR24092, PTHR24092, 1 hit PTHR24092:SF48, PTHR24092:SF48, 1 hit |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AT8B1_XENTR | |
Accessioni | Q5BL50Primary (citable) accession number: Q5BL50 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | April 12, 2005 | |
Last modified: | April 7, 2021 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families