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UniProtKB - Q5BKT4 (AG10A_HUMAN)
Protein
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
Gene
ALG10
Organism
Homo sapiens (Human)
Status
Functioni
Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc2-PP-Dol.
Catalytic activityi
- α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl β-D-glucosyl phosphate = α-D-Glc-(1→2)-α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl phosphate + H+EC:2.4.1.256
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
GO - Biological processi
- dolichol-linked oligosaccharide biosynthetic process Source: InterPro
- protein N-linked glycosylation Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
PathwayCommonsi | Q5BKT4 |
Reactomei | R-HSA-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
SignaLinki | Q5BKT4 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT59, Glycosyltransferase Family 59 |
Names & Taxonomyi
Protein namesi | Recommended name: Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (EC:2.4.1.256)Alternative name(s): Alpha-1,2-glucosyltransferase ALG10-A Alpha-2-glucosyltransferase ALG10-A Asparagine-linked glycosylation protein 10 homolog A |
Gene namesi | Name:ALG10 Synonyms:ALG10A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:23162, ALG10 |
MIMi | 618355, gene |
neXtProti | NX_Q5BKT4 |
VEuPathDBi | HostDB:ENSG00000139133 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 7 – 27 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 28 – 64 | ExtracellularSequence analysisAdd BLAST | 37 | |
Transmembranei | 65 – 85 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 86 – 97 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 98 – 118 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 119 – 130 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 131 – 151 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 152 – 172 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 173 – 175 | ExtracellularSequence analysis | 3 | |
Transmembranei | 176 – 196 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 197 – 249 | CytoplasmicSequence analysisAdd BLAST | 53 | |
Transmembranei | 250 – 270 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 271 – 283 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 284 – 304 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 305 – 323 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 324 – 344 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 345 – 367 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 368 – 388 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 389 – 392 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 393 – 413 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 414 – 436 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 437 – 457 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 458 – 473 | CytoplasmicSequence analysisAdd BLAST | 16 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 84920 |
OpenTargetsi | ENSG00000139133 |
PharmGKBi | PA134732019 |
Miscellaneous databases
Pharosi | Q5BKT4, Tbio |
Genetic variation databases
BioMutai | ALG10 |
DMDMi | 74736030 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215447 | 1 – 473 | Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseAdd BLAST | 473 |
Proteomic databases
EPDi | Q5BKT4 |
jPOSTi | Q5BKT4 |
MassIVEi | Q5BKT4 |
MaxQBi | Q5BKT4 |
PaxDbi | Q5BKT4 |
PeptideAtlasi | Q5BKT4 |
PRIDEi | Q5BKT4 |
ProteomicsDBi | 62698 |
PTM databases
iPTMneti | Q5BKT4 |
PhosphoSitePlusi | Q5BKT4 |
Expressioni
Gene expression databases
Bgeei | ENSG00000139133, Expressed in heart and 141 other tissues |
ExpressionAtlasi | Q5BKT4, baseline and differential |
Genevisiblei | Q5BKT4, HS |
Organism-specific databases
HPAi | ENSG00000139133, Low tissue specificity |
Interactioni
Binary interactionsi
Q5BKT4
Protein-protein interaction databases
BioGRIDi | 124356, 44 interactors |
IntActi | Q5BKT4, 20 interactors |
STRINGi | 9606.ENSP00000266483 |
Miscellaneous databases
RNActi | Q5BKT4, protein |
Structurei
Family & Domainsi
Sequence similaritiesi
Belongs to the ALG10 glucosyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2642, Eukaryota |
GeneTreei | ENSGT00390000012906 |
HOGENOMi | CLU_017053_1_0_1 |
InParanoidi | Q5BKT4 |
OMAi | IWVAFCA |
OrthoDBi | 476469at2759 |
PhylomeDBi | Q5BKT4 |
TreeFami | TF300150 |
Family and domain databases
InterProi | View protein in InterPro IPR016900, Alg10 |
PANTHERi | PTHR12989, PTHR12989, 1 hit |
Pfami | View protein in Pfam PF04922, DIE2_ALG10, 1 hit |
PIRSFi | PIRSF028810, Alpha1_2_glucosyltferase_Alg10, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q5BKT4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQLEGYYFS AALSCTFLVS CLLFSAFSRA LREPYMDEIF HLPQAQRYCE
60 70 80 90 100
GHFSLSQWDP MITTLPGLYL VSIGVIKPAI WIFGWSEHVV CSIGMLRFVN
110 120 130 140 150
LLFSVGNFYL LYLLFCKVQP RNKAASSIQR VLSTLTLAVF PTLYFFNFLY
160 170 180 190 200
YTEAGSMFFT LFAYLMCLYG NHKTSAFLGF CGFMFRQTNI IWAVFCAGNV
210 220 230 240 250
IAQKLTEAWK TELQKKEDRL PPIKGPFAEF RKILQFLLAY SMSFKNLSML
260 270 280 290 300
LLLTWPYILL GFLFCAFVVV NGGIVIGDRS SHEACLHFPQ LFYFFSFTLF
310 320 330 340 350
FSFPHLLSPS KIKTFLSLVW KRRILFFVVT LVSVFLVWKF TYAHKYLLAD
360 370 380 390 400
NRHYTFYVWK RVFQRYETVK YLLVPAYIFA GWSIADSLKS KSIFWNLMFF
410 420 430 440 450
ICLFTVIVPQ KLLEFRYFIL PYVIYRLNIP LPPTSRLICE LSCYAVVNFI
460 470
TFFIFLNKTF QWPNSQDIQR FMW
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5GZZ6 | F5GZZ6_HUMAN | Dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2... | ALG10 | 126 | Annotation score: | ||
F5H5T2 | F5H5T2_HUMAN | Dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2... | ALG10 | 77 | Annotation score: |
Sequence cautioni
The sequence BAB55272 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 184 | M → V in AAH70347 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 258 | I → T in CAC41349 (PubMed:12200473).Curated | 1 | |
Sequence conflicti | 384 | I → T in BAB55272 (PubMed:14702039).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ312278 mRNA Translation: CAC41349.1 BC070347 mRNA Translation: AAH70347.1 BC090948 mRNA Translation: AAH90948.1 AK027657 mRNA Translation: BAB55272.1 Different initiation. |
CCDSi | CCDS41769.1 |
RefSeqi | NP_116223.3, NM_032834.3 |
Genome annotation databases
Ensembli | ENST00000266483; ENSP00000266483; ENSG00000139133 |
GeneIDi | 84920 |
KEGGi | hsa:84920 |
MANE-Selecti | ENST00000266483.7; ENSP00000266483.2; NM_032834.4; NP_116223.3 |
UCSCi | uc001rlm.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ312278 mRNA Translation: CAC41349.1 BC070347 mRNA Translation: AAH70347.1 BC090948 mRNA Translation: AAH90948.1 AK027657 mRNA Translation: BAB55272.1 Different initiation. |
CCDSi | CCDS41769.1 |
RefSeqi | NP_116223.3, NM_032834.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 124356, 44 interactors |
IntActi | Q5BKT4, 20 interactors |
STRINGi | 9606.ENSP00000266483 |
Protein family/group databases
CAZyi | GT59, Glycosyltransferase Family 59 |
PTM databases
iPTMneti | Q5BKT4 |
PhosphoSitePlusi | Q5BKT4 |
Genetic variation databases
BioMutai | ALG10 |
DMDMi | 74736030 |
Proteomic databases
EPDi | Q5BKT4 |
jPOSTi | Q5BKT4 |
MassIVEi | Q5BKT4 |
MaxQBi | Q5BKT4 |
PaxDbi | Q5BKT4 |
PeptideAtlasi | Q5BKT4 |
PRIDEi | Q5BKT4 |
ProteomicsDBi | 62698 |
Protocols and materials databases
Antibodypediai | 55444, 90 antibodies from 18 providers |
DNASUi | 84920 |
Genome annotation databases
Ensembli | ENST00000266483; ENSP00000266483; ENSG00000139133 |
GeneIDi | 84920 |
KEGGi | hsa:84920 |
MANE-Selecti | ENST00000266483.7; ENSP00000266483.2; NM_032834.4; NP_116223.3 |
UCSCi | uc001rlm.4, human |
Organism-specific databases
CTDi | 84920 |
DisGeNETi | 84920 |
GeneCardsi | ALG10 |
HGNCi | HGNC:23162, ALG10 |
HPAi | ENSG00000139133, Low tissue specificity |
MIMi | 618355, gene |
neXtProti | NX_Q5BKT4 |
OpenTargetsi | ENSG00000139133 |
PharmGKBi | PA134732019 |
VEuPathDBi | HostDB:ENSG00000139133 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2642, Eukaryota |
GeneTreei | ENSGT00390000012906 |
HOGENOMi | CLU_017053_1_0_1 |
InParanoidi | Q5BKT4 |
OMAi | IWVAFCA |
OrthoDBi | 476469at2759 |
PhylomeDBi | Q5BKT4 |
TreeFami | TF300150 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
PathwayCommonsi | Q5BKT4 |
Reactomei | R-HSA-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
SignaLinki | Q5BKT4 |
Miscellaneous databases
BioGRID-ORCSi | 84920, 51 hits in 974 CRISPR screens |
ChiTaRSi | ALG10, human |
GenomeRNAii | 84920 |
Pharosi | Q5BKT4, Tbio |
PROi | PR:Q5BKT4 |
RNActi | Q5BKT4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000139133, Expressed in heart and 141 other tissues |
ExpressionAtlasi | Q5BKT4, baseline and differential |
Genevisiblei | Q5BKT4, HS |
Family and domain databases
InterProi | View protein in InterPro IPR016900, Alg10 |
PANTHERi | PTHR12989, PTHR12989, 1 hit |
Pfami | View protein in Pfam PF04922, DIE2_ALG10, 1 hit |
PIRSFi | PIRSF028810, Alpha1_2_glucosyltferase_Alg10, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AG10A_HUMAN | |
Accessioni | Q5BKT4Primary (citable) accession number: Q5BKT4 Secondary accession number(s): Q6NS98, Q96DU0, Q96SM6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | April 12, 2005 | |
Last modified: | February 23, 2022 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families