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Protein

Ribonuclease H1

Gene

Rnaseh1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II.By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.By similarity

Activity regulationi

In the presence of magnesium, manganese is inhibitory.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144Magnesium 1PROSITE-ProRule annotation1
Metal bindingi144Magnesium 2PROSITE-ProRule annotation1
Metal bindingi185Magnesium 1PROSITE-ProRule annotation1
Metal bindingi209Magnesium 1PROSITE-ProRule annotation1
Metal bindingi273Magnesium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H1 (EC:3.1.26.4)
Short name:
RNase H1
Gene namesi
Name:Rnaseh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi1309012 Rnaseh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001954351 – 285Ribonuclease H1Add BLAST285

Proteomic databases

PaxDbiQ5BK46
PRIDEiQ5BK46

Expressioni

Gene expression databases

BgeeiENSRNOG00000008584 Expressed in 10 organ(s), highest expression level in spleen
GenevisibleiQ5BK46 RN

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011819

Structurei

3D structure databases

ProteinModelPortaliQ5BK46
SMRiQ5BK46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini135 – 281RNase HPROSITE-ProRule annotationAdd BLAST147

Sequence similaritiesi

Belongs to the RNase H family.Curated

Phylogenomic databases

eggNOGiKOG3752 Eukaryota
COG0328 LUCA
GeneTreeiENSGT00390000003466
HOGENOMiHOG000040465
HOVERGENiHBG002135
InParanoidiQ5BK46
KOiK03469
OMAiELWYGLY
OrthoDBiEOG091G0RCI
PhylomeDBiQ5BK46
TreeFamiTF313356

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.40.970.10, 1 hit
InterProiView protein in InterPro
IPR009027 Ribosomal_L9/RNase_H1_N
IPR017067 RNase_H1_euk
IPR011320 RNase_H1_N
IPR037056 RNase_H1_N_sf
IPR012337 RNaseH-like_sf
IPR002156 RNaseH_domain
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF01693 Cauli_VI, 1 hit
PF00075 RNase_H, 1 hit
PIRSFiPIRSF036852 Ribonuclease_H1_euk, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
SSF55658 SSF55658, 1 hit
PROSITEiView protein in PROSITE
PS50879 RNASE_H, 1 hit

Sequencei

Sequence statusi: Complete.

Q5BK46-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRWLLPLFRT VTLAVVRVRR GVCGLGMFYA VRRGRRTGVF LSWSECKAQV
60 70 80 90 100
DRFPAARFKK FATEDEAWAF VRSSSSPDGS KGQESAHVQK LQVKTSKRPR
110 120 130 140 150
EPLGEEEEPP EPGAKHTRQD TEPAALVSKD AFSYMGESVV VYTDGCCSSN
160 170 180 190 200
GRKRARAGIG VYWGPGHPLN VGIRLPGRQT NQRAEIHAAC KAITQAKAQN
210 220 230 240 250
ISKLVLYTDS MFTINGITNW VQGWKKNGWR TSTGKDVINK EDFMELDELT
260 270 280
QGMDIQWMHI PGHSGFVGNE EADRLAREGA KQSEG
Length:285
Mass (Da):31,773
Last modified:April 12, 2005 - v1
Checksum:iDC065C7F06EA1807
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091209 mRNA Translation: AAH91209.1
RefSeqiNP_001013115.1, NM_001013097.2
NP_001273867.1, NM_001286938.1
UniGeneiRn.13139

Genome annotation databases

EnsembliENSRNOT00000011819; ENSRNOP00000011819; ENSRNOG00000008584
GeneIDi298933
KEGGirno:298933

Similar proteinsi

Entry informationi

Entry nameiRNH1_RAT
AccessioniPrimary (citable) accession number: Q5BK46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: April 12, 2005
Last modified: September 12, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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