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Protein

Endo-beta-1,4-glucanase celB

Gene

celB

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 5.5.1 Publication

Temperature dependencei

Optimum temperature is 42 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei216NucleophileBy similarity1
Active sitei221Proton donorBy similarity1

GO - Molecular functioni

  • cellulase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH7 Glycoside Hydrolase Family 7
mycoCLAPiEGL7A_EMENI

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-beta-1,4-glucanase celB (EC:3.2.1.4)
Short name:
Endoglucanase celB
Alternative name(s):
Carboxymethylcellulase celB
Cellulase B
Gene namesi
Name:celB
Synonyms:eglB, eglC
ORF Names:AN3418
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VI
  • UP000005890 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000039515917 – 430Endo-beta-1,4-glucanase celBAdd BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi43N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi395N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ5B7R2

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00009621

Structurei

3D structure databases

ProteinModelPortaliQ5B7R2
SMRiQ5B7R2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000182210
InParanoidiQ5B7R2
KOiK19357
OMAiPWINGEA
OrthoDBiEOG092C29WB

Family and domain databases

CDDicd07999 GH7_CBH_EG, 1 hit
Gene3Di2.70.100.10, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001722 Glyco_hydro_7
IPR037019 Glyco_hydro_7_sf
PANTHERiPTHR33753 PTHR33753, 1 hit
PfamiView protein in Pfam
PF00840 Glyco_hydro_7, 1 hit
PRINTSiPR00734 GLHYDRLASE7
SUPFAMiSSF49899 SSF49899, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5B7R2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALLLSLSLL ATTISAQQIG TPEIRPRLTT YHCTSANGCT EQNTSVVLDA
60 70 80 90 100
ATHPIHDASN PSVSCTTSNG LNPALCPDKQ TCADNCVIDG ITDYAAHGVE
110 120 130 140 150
THGSRLTLTQ YRNVNGALSS VSPRVYLVDE SDPDEQEYRA LSLLAQEFTF
160 170 180 190 200
TVNVSALPCG MNGALYLSEM SPSGGRSALN PAGASYGTGY CDAQCYVNPW
210 220 230 240 250
INGEGNINGY GACCNEMDIW EANSRSTGFT PHACLYEPEE TEGRGVYECA
260 270 280 290 300
SEDECDSAGE NDGICDKWGC GFNPYALGNT EYYGRGQGFE VDTKEPFTVV
310 320 330 340 350
TQFLTDDGTS TGALTEIRRL YIQNGQVIEN AVVSSGADSL TDSLCASTAS
360 370 380 390 400
WFDSYGGMEG MGRALGRGMV LAMSIWNDAG GYMQWLDGGD AGPCNATEGA
410 420 430
PEFIEEHTPW TRVVFEDLKW GDIGSTFQAS
Length:430
Mass (Da):45,929
Last modified:April 26, 2005 - v1
Checksum:i4807FA907F18749E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45S → Y in AAM54071 (PubMed:12409103).Curated1
Sequence conflicti73P → R in AAM54071 (PubMed:12409103).Curated1
Sequence conflicti113N → Y in AAM54071 (PubMed:12409103).Curated1
Sequence conflicti153N → Y in AAM54071 (PubMed:12409103).Curated1
Sequence conflicti316E → D in AAM54071 (PubMed:12409103).Curated1
Sequence conflicti345C → W in AAM54071 (PubMed:12409103).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420021 Genomic DNA Translation: AAM54071.1
AACD01000055 Genomic DNA Translation: EAA63386.1
BN001306 Genomic DNA Translation: CBF82749.1
RefSeqiXP_661022.1, XM_655930.1

Genome annotation databases

EnsemblFungiiCBF82749; CBF82749; ANIA_03418
EAA63386; EAA63386; AN3418.2
GeneIDi2874128
KEGGiani:AN3418.2

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF420021 Genomic DNA Translation: AAM54071.1
AACD01000055 Genomic DNA Translation: EAA63386.1
BN001306 Genomic DNA Translation: CBF82749.1
RefSeqiXP_661022.1, XM_655930.1

3D structure databases

ProteinModelPortaliQ5B7R2
SMRiQ5B7R2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi162425.CADANIAP00009621

Protein family/group databases

CAZyiGH7 Glycoside Hydrolase Family 7
mycoCLAPiEGL7A_EMENI

Proteomic databases

PRIDEiQ5B7R2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCBF82749; CBF82749; ANIA_03418
EAA63386; EAA63386; AN3418.2
GeneIDi2874128
KEGGiani:AN3418.2

Phylogenomic databases

HOGENOMiHOG000182210
InParanoidiQ5B7R2
KOiK19357
OMAiPWINGEA
OrthoDBiEOG092C29WB

Family and domain databases

CDDicd07999 GH7_CBH_EG, 1 hit
Gene3Di2.70.100.10, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001722 Glyco_hydro_7
IPR037019 Glyco_hydro_7_sf
PANTHERiPTHR33753 PTHR33753, 1 hit
PfamiView protein in Pfam
PF00840 Glyco_hydro_7, 1 hit
PRINTSiPR00734 GLHYDRLASE7
SUPFAMiSSF49899 SSF49899, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCELB_EMENI
AccessioniPrimary (citable) accession number: Q5B7R2
Secondary accession number(s): C8VHK3, Q8NK01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: April 26, 2005
Last modified: June 20, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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