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UniProtKB - Q5AMQ4 (CEGT_CANAL)
Protein
Ceramide glucosyltransferase
Gene
HSX11
Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Functioni
Catalyzes the final step in the biosynthesis of the membrane lipid glucosylceramide (GluCer), the transfer of glucose to ceramide. Glucosylceramides play important roles in growth, differentiation and pathogenicity.
1 PublicationCatalytic activityi
- an N-acylsphing-4-enine + UDP-α-D-glucose = a β-D-glucosyl-(1↔1ʼ)-N-acylsphing-4-enine + H+ + UDP1 PublicationEC:2.4.1.801 Publication
: sphingolipid metabolism Pathwayi
This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 364 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- ceramide glucosyltransferase activity Source: CGD
- dihydroceramide glucosyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- filamentous growth Source: CGD
- glucosylceramide biosynthetic process Source: CGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Lipid metabolism, Sphingolipid metabolism |
Enzyme and pathway databases
UniPathwayi | UPA00222 |
Names & Taxonomyi
Protein namesi | Recommended name: Ceramide glucosyltransferaseBy similarity (EC:2.4.1.801 Publication)Alternative name(s): GLCT-1 Glucosylceramide synthase1 Publication Short name: GCS UDP-glucose ceramide glucosyltransferase1 Publication UDP-glucose:N-acylsphingosine D-glucosyltransferase |
Gene namesi | Ordered Locus Names:CAALFM_C402010CA ORF Names:CaO19.12061, CaO19.4592 |
Organismi | Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) |
Taxonomic identifieri | 237561 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Candida/Lodderomyces clade › Candida › |
Proteomesi |
|
Organism-specific databases
CGDi | CAL0000175173, HSX11 |
VEuPathDBi | FungiDB:C4_02010C_A |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein Sequence analysis
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 15 | LumenalCuratedAdd BLAST | 15 | |
Transmembranei | 16 – 36 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 37 – 427 | CytoplasmicBy similarityAdd BLAST | 391 | |
Transmembranei | 428 – 448 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 449 – 451 | LumenalCurated | 3 | |
Transmembranei | 452 – 472 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 473 – 501 | CytoplasmicCuratedAdd BLAST | 29 | |
Transmembranei | 502 – 522 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 523 – 544 | LumenalCuratedAdd BLAST | 22 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Results in complete loss of glucosylceramides (GluCers) in mutant cells (PubMed:11443131). Has a decreased hyphal growth rate and increased susceptibility to SDS and fluconazole, suggesting defects in the cell membrane structure (PubMed:20019081). Shows increased resistance to plant defensin RsAFP2, and to heliomicin, a defensin-like peptide from the insect Heliothis virescens (PubMed:14604982).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000434806 | 1 – 544 | Ceramide glucosyltransferaseAdd BLAST | 544 |
Structurei
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 109 | D1Curated | 1 | |
Motifi | 171 | D2Curated | 1 | |
Motifi | 364 | D3Curated | 1 | |
Motifi | 404 – 408 | (Q/R)XXRWCurated | 5 |
Domaini
The D1, D2, D3, (Q/R)XXRW motif is a critical part of the GCS active site, involved in catalysis and UDP-sugar binding.By similarity
Sequence similaritiesi
Belongs to the glycosyltransferase 2 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2547, Eukaryota |
HOGENOMi | CLU_030898_1_0_1 |
InParanoidi | Q5AMQ4 |
OMAi | HGSMPFH |
OrthoDBi | 793389at2759 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR025993, Ceramide_glucosylTrfase IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR12726, PTHR12726, 1 hit |
Pfami | View protein in Pfam PF13506, Glyco_transf_21, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5AMQ4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVQEELSLFR ITTGYFFLLW YIIILVAAYS GFFEILFNFR NRPILHTKQQ
60 70 80 90 100
ANHQNDPESD DEEIYEGVTI IRPIKGIDPE LTSCLESSFC QNYPRSKLQI
110 120 130 140 150
LFCVDDPNDP SIPIIQKLIA KYPTVDAQIL TSESYNSQTK TSDDHYGPNP
160 170 180 190 200
KVNNLAKGFV HAKYDILWVM DSNVWASSNI LKNSVISLNG NLNMSRKMGQ
210 220 230 240 250
SRPVKLVHHV PLALSINNTT RSDDFIGGQD LEITAMTPVP SSESLNSQLV
260 270 280 290 300
KRKSSPKSNN SLNVHPGFTY SKFSKKLGAE LDEMFLHTSH SKFYVSLNNL
310 320 330 340 350
AVAPCVNGKS NIYRRSDLDQ SVRLIPHKDS PFFKDPKVKQ DAGYYTSLGV
360 370 380 390 400
GHAIKFFARY IGEDNMIGIA LWENTQGRTG LTGDVVVQPY SGSENNAVKD
410 420 430 440 450
YIQRRVRWLR VRKYMVLLAT LIEPTTESII CGIYGTYAIS TVFFGTWFNK
460 470 480 490 500
YWFVMHMLIW MLTDYVQYHT LINHTLDVKN ITYLPNWLNE SIPPKQRNCL
510 520 530 540
QWGYIWILRE LLALPIWIIA MIGHEIDWRG RPFRIKKDLT AEEM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP017626 Genomic DNA Translation: AOW28972.1 |
RefSeqi | XP_722664.1, XM_717571.1 |
Genome annotation databases
EnsemblFungii | KHC77503; KHC77503; W5Q_03501 KHC86755; KHC86755; I503_03493 |
GeneIDi | 3635660 |
KEGGi | cal:CAALFM_C402010CA |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP017626 Genomic DNA Translation: AOW28972.1 |
RefSeqi | XP_722664.1, XM_717571.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 237561.Q5AMQ4 |
Genome annotation databases
EnsemblFungii | KHC77503; KHC77503; W5Q_03501 KHC86755; KHC86755; I503_03493 |
GeneIDi | 3635660 |
KEGGi | cal:CAALFM_C402010CA |
Organism-specific databases
CGDi | CAL0000175173, HSX11 |
VEuPathDBi | FungiDB:C4_02010C_A |
Phylogenomic databases
eggNOGi | KOG2547, Eukaryota |
HOGENOMi | CLU_030898_1_0_1 |
InParanoidi | Q5AMQ4 |
OMAi | HGSMPFH |
OrthoDBi | 793389at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00222 |
Miscellaneous databases
PROi | PR:Q5AMQ4 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR025993, Ceramide_glucosylTrfase IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR12726, PTHR12726, 1 hit |
Pfami | View protein in Pfam PF13506, Glyco_transf_21, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CEGT_CANAL | |
Accessioni | Q5AMQ4Primary (citable) accession number: Q5AMQ4 Secondary accession number(s): A0A1D8PLE8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 9, 2015 |
Last sequence update: | April 26, 2005 | |
Last modified: | February 23, 2022 | |
This is version 83 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Candida albicans
Candida albicans: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families