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UniProtKB - Q59I44 (CAA43_BURSP)
Protein
2-haloacrylate reductase
Gene
caa43
Organism
Burkholderia sp.
Status
Functioni
Involved in the degradation of unsaturated organohalogen compounds. Catalyzes the NADPH-dependent reduction of the carbon-carbon double bond of 2-chloroacrylate to produce (S)-2-chloropropionate, which is probably further metabolized to (R)-lactate by (S)-2-haloacid dehalogenase. Can also use 2-bromoacrylate as substrate. Does not act on acrylate, methacrylate, 1,4-benzoquinone and 1,4-naphthoquinone.
1 PublicationCatalytic activityi
- EC:1.3.1.1031 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 153 – 159 | NADP1 Publication | 7 |
GO - Molecular functioni
- 2-chloroacrylate reductase activity Source: UniProtKB-EC
- oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor Source: UniProtKB
GO - Biological processi
- halogenated hydrocarbon catabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.3.1.103, 1033 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:caa431 Publication |
Organismi | Burkholderia sp. |
Taxonomic identifieri | 36773 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › |
Pathology & Biotechi
Biotechnological usei
(S)-2-chloropropionate is used in a large quantity as a synthetic precursor for the industrial production of aryloxyphenoxypropionic acid herbicides.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000430752 | 2 – 333 | 2-haloacrylate reductase1 PublicationAdd BLAST | 332 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q59I44 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q59I44 |
Family & Domainsi
Sequence similaritiesi
Belongs to the zinc-containing alcohol dehydrogenase family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH_C IPR013154, ADH_N IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q59I44-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVMAAVIHKK GGPDNFVWEE VKVGSPGPGQ VRLRNTAIGV NFLDTYHRAG
60 70 80 90 100
IPHPLVVGEP PIVVGFEAAA VVEEVGPGVT DFTVGERVCT CLPPLGAYSQ
110 120 130 140 150
ERLYPAEKLI KVPKDLDLDD VHLAGLMLKG MTAQYLLHQT HKVKPGDYVL
160 170 180 190 200
IHAAAGGMGH IMVPWARHLG ATVIGTVSTE EKAETARKLG CHHTINYSTQ
210 220 230 240 250
DFAEVVREIT GGKGVDVVYD SIGKDTLQKS LDCLRPRGMC AAYGHASGVA
260 270 280 290 300
DPIRVVEDLG VRGSLFITRP ALWHYMSNRS EIDEGSKCLF DAVKAGVLHS
310 320 330
SVAKTFPLRE AAAAHKYMGG RQTIGSIVLL PQA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB196962 Genomic DNA Translation: BAD91551.1 |
Genome annotation databases
KEGGi | ag:BAD91551 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB196962 Genomic DNA Translation: BAD91551.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WLY | X-ray | 1.30 | A | 1-333 | [»] | |
SMRi | Q59I44 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
KEGGi | ag:BAD91551 |
Enzyme and pathway databases
BRENDAi | 1.3.1.103, 1033 |
Miscellaneous databases
EvolutionaryTracei | Q59I44 |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH_C IPR013154, ADH_N IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAA43_BURSP | |
Accessioni | Q59I44Primary (citable) accession number: Q59I44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 29, 2014 |
Last sequence update: | April 26, 2005 | |
Last modified: | February 23, 2022 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families