TNNI3K

Functionⁱ

May play a role in cardiac physiology.1 Publication

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactorⁱ

Mg²⁺1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	490	ATP		1
Active siteⁱ	588	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	469 – 477	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
metal ion binding Source: UniProtKB-KW
protein C-terminus binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 18205602
protein kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 12721663
protein serine/threonine kinase activity Source: UniProtKB-KW
troponin I binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 12721663

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

bundle of His cell to Purkinje myocyte communication
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 23236294
intracellular signal transduction Source: GO_Central
protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 12721663
regulation of cardiac conduction
Source: BHF-UCL
Inferred from genetic interactionⁱ
- 23236294
regulation of cardiac muscle contraction
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 24925317
regulation of heart rate
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 24925317

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Serine/threonine-protein kinase TNNI3K (EC:2.7.11.1) Alternative name(s): Cardiac ankyrin repeat kinase Cardiac troponin I-interacting kinase TNNI3-interacting kinase
Gene namesⁱ	Name:TNNI3KImported Synonyms:CARK
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000116783.14
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:19661 TNNI3K
MIMⁱ	613932 gene
neXtProtⁱ	NX_Q59H18

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Involvement in diseaseⁱ

Cardiac conduction disease with or without dilated cardiomyopathy (CCDD)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA cardiac disorder characterized by atrial tachyarrhythmia and conduction system disease. Some patients have dilated cardiomyopathy.

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_072650	526	G → D in CCDD; the mutation results in decreased protein solubility; causes abnormal aggregation; markedly reduced protein expression is observed in the sarcoplasm and nuclei of patient cardiomyocytes. 1 PublicationCorresponds to variant dbSNP:rs606231469EnsemblClinVar.		1

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	490	K → R: Loss of autophosphorylation activity. 1 Publication		1

Keywords - Diseaseⁱ

Cardiomyopathy, Disease mutation

Organism-specific databases

DisGeNET More...DisGeNETⁱ	51086
MalaCards human disease database More...MalaCardsⁱ	TNNI3K
MIMⁱ	616117 phenotype
Open Targets More...OpenTargetsⁱ	ENSG00000116783 ENSG00000259030
PharmGKBⁱ	PA134976654

Chemistry databases

ChEMBLⁱ	CHEMBL5260

ChEMBLⁱ

CHEMBL5260

Polymorphism and mutation databases

BioMutaⁱ	TNNI3K
DMDMⁱ	300669705

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000086757	1 – 835	Serine/threonine-protein kinase TNNI3KAdd BLAST		835

Post-translational modificationⁱ

Autophosphorylated.1 Publication

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q59H18
PeptideAtlas More...PeptideAtlasⁱ	Q59H18
PRIDEⁱ	Q59H18
ProteomicsDBⁱ	62665 62666 [Q59H18-1] 62667 [Q59H18-3] 62668 [Q59H18-4]

PTM databases

iPTMnetⁱ	Q59H18
PhosphoSitePlusⁱ	Q59H18

Expressionⁱ

Tissue specificityⁱ

Highly expressed in both adult and fetal heart.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000116783 Expressed in 88 organ(s), highest expression level in heart
CleanExⁱ	HS_TNNI3K
ExpressionAtlasⁱ	Q59H18 baseline and differential
Genevisibleⁱ	Q59H18 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA072331

HPAⁱ

HPA072331

Interactionⁱ

Subunit structureⁱ

Interacts with TNNI3, ACTC, ACTA1, MYBPC3, AIP, BABP3 and HADHB.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
TNNI3	P19429	2	EBI-704142,EBI-704146

With

Entry

#Exp.

IntAct

Notes

TNNI3

P19429

2

EBI-704142,EBI-704146

GO - Molecular functionⁱ

protein C-terminus binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 18205602
troponin I binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 12721663

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	119276, 11 interactors
IntActⁱ	Q59H18, 8 interactors
STRINGⁱ	9606.ENSP00000450895

Chemistry databases

BindingDBⁱ

Q59H18

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	442 – 451	Combined sources	10
Helixⁱ	454 – 456	Combined sources	3
Helixⁱ	460 – 462	Combined sources	3
Beta strandⁱ	463 – 470	Combined sources	8
Beta strandⁱ	473 – 482	Combined sources	10
Beta strandⁱ	485 – 492	Combined sources	8
Helixⁱ	502 – 515	Combined sources	14
Beta strandⁱ	524 – 528	Combined sources	5
Helixⁱ	532 – 534	Combined sources	3
Beta strandⁱ	536 – 540	Combined sources	5
Helixⁱ	547 – 552	Combined sources	6
Helixⁱ	560 – 578	Combined sources	19
Beta strandⁱ	580 – 582	Combined sources	3
Helixⁱ	591 – 593	Combined sources	3
Beta strandⁱ	594 – 596	Combined sources	3
Beta strandⁱ	602 – 604	Combined sources	3
Helixⁱ	628 – 630	Combined sources	3
Helixⁱ	633 – 636	Combined sources	4
Helixⁱ	637 – 639	Combined sources	3
Helixⁱ	645 – 660	Combined sources	16
Turnⁱ	664 – 667	Combined sources	4
Helixⁱ	670 – 678	Combined sources	9
Helixⁱ	692 – 701	Combined sources	10
Helixⁱ	706 – 708	Combined sources	3
Helixⁱ	712 – 725	Combined sources	14

Show more details

3D structure databases

ProteinModelPortalⁱ	Q59H18
SMRⁱ	Q59H18
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Repeatⁱ	66 – 96	ANK 1Add BLAST	31
Repeatⁱ	100 – 129	ANK 2Add BLAST	30
Repeatⁱ	133 – 162	ANK 3Add BLAST	30
Repeatⁱ	166 – 195	ANK 4Add BLAST	30
Repeatⁱ	199 – 228	ANK 5Add BLAST	30
Repeatⁱ	234 – 263	ANK 6Add BLAST	30
Repeatⁱ	269 – 298	ANK 7Add BLAST	30
Repeatⁱ	304 – 335	ANK 8Add BLAST	32
Repeatⁱ	339 – 368	ANK 9Add BLAST	30
Repeatⁱ	381 – 410	ANK 10Add BLAST	30
Domainⁱ	463 – 723	Protein kinasePROSITE-ProRule annotationAdd BLAST	261

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	21 – 51	Sequence analysisAdd BLAST		31

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	733 – 746	Poly-SerAdd BLAST		14

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Curated

Keywords - Domainⁱ

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGⁱ	ENOG410IT5N Eukaryota COG0666 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140790
HOGENOMⁱ	HOG000234421
HOVERGENⁱ	HBG079513
InParanoidⁱ	Q59H18
KEGG Orthology (KO) More...KOⁱ	K17535
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G01QL
PhylomeDBⁱ	Q59H18

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00204 ANK, 5 hits
Gene3Dⁱ	1.25.40.20, 4 hits
InterProⁱ	View protein in InterPro IPR002110 Ankyrin_rpt IPR020683 Ankyrin_rpt-contain_dom IPR036770 Ankyrin_rpt-contain_sf IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12796 Ank_2, 3 hits PF07714 Pkinase_Tyr, 1 hit
PRINTSⁱ	PR01415 ANKYRIN
SMARTⁱ	View protein in SMART SM00248 ANK, 10 hits
SUPFAMⁱ	SSF48403 SSF48403, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS50297 ANK_REP_REGION, 1 hit PS50088 ANK_REPEAT, 6 hits PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (4+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show all Align All

Isoform 21 Publication (identifier: Q59H18-2) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNYKSRPTQ TCTDEWKKKV SESYVITIER LEDDLQIKEK ELTELRNIFG 
        60         70         80         90        100
SDEAFSKVNL NYRTENGLSL LHLCCICGGK KSHIRTLMLK GLRPSRLTRN 
       110        120        130        140        150
GFTALHLAVY KDNAELITSL LHSGADIQQV GYGGLTALHI ATIAGHLEAA 
       160        170        180        190        200
DVLLQHGANV NIQDAVFFTP LHIAAYYGHE QVTRLLLKFG ADVNVSGEVG 
       210        220        230        240        250
DRPLHLASAK GFLNIAKLLM EEGSKADVNA QDNEDHVPLH FCSRFGHHDI 
       260        270        280        290        300
VKYLLQSDLE VQPHVVNIYG DTPLHLACYN GKFEVAKEII QISGTESLTK 
       310        320        330        340        350
ENIFSETAFH SACTYGKSID LVKFLLDQNV ININHQGRDG HTGLHSACYH 
       360        370        380        390        400
GHIRLVQFLL DNGADMNLVA CDPSRSSGEK DEQTCLMWAY EKGHDAIVTL 
       410        420        430        440        450
LKHYKRPQDE LPCNEYSQPG GDGSYVSVPS PLGKIKSMTK EKADILLLRA 
       460        470        480        490        500
GLPSHFHLQL SEIEFHEIIG SGSFGKVYKG RCRNKIVAIK RYRANTYCSK 
       510        520        530        540        550
SDVDMFCREV SILCQLNHPC VIQFVGACLN DPSQFAIVTQ YISGGSLFSL 
       560        570        580        590        600
LHEQKRILDL QSKLIIAVDV AKGMEYLHNL TQPIIHRDLN SHNILLYEDG 
       610        620        630        640        650
HAVVADFGES RFLQSLDEDN MTKQPGNLRW MAPEVFTQCT RYTIKADVFS 
       660        670        680        690        700
YALCLWEILT GEIPFAHLKP AAAAADMAYH HIRPPIGYSI PKPISSLLIR 
       710        720        730        740        750
GWNACPEGRP EFSEVVMKLE ECLCNIELMS PASSNSSGSL SPSSSSDCLV 
       760        770        780        790        800
NRGGPGRSHV AALRSRFELE YALNARSYAA LSQSAGQYSS QGLSLEEMKR 
       810        820        830 
SLQYTPIDKY GYVSDPMSSM HFHSCRNSSS FEDSS

Length:835

Mass (Da):92,851

Last modified:July 13, 2010 - v3

Checksum:ⁱ3B21484B434F46E8

GO

Isoform 1Curated (identifier: Q59H18-1) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-13: MGNYKSRPTQTCT → MAAARDPPEV...NSFTILLIHS

« Hide

        10         20         30         40         50
MAAARDPPEV SLREATQRKL RRFSELRGKL VARGEFWDIV AITAADEKQE 
        60         70         80         90        100
LAYNQQLSEK LKRKELPLGV QYHVFVDPAG AKIGNGGSTL CALQCLEKLY 
       110        120        130        140        150
GDKWNSFTIL LIHSDEWKKK VSESYVITIE RLEDDLQIKE KELTELRNIF 
       160        170        180        190        200
GSDEAFSKVN LNYRTENGLS LLHLCCICGG KKSHIRTLML KGLRPSRLTR 
       210        220        230        240        250
NGFTALHLAV YKDNAELITS LLHSGADIQQ VGYGGLTALH IATIAGHLEA 
       260        270        280        290        300
ADVLLQHGAN VNIQDAVFFT PLHIAAYYGH EQVTRLLLKF GADVNVSGEV 
       310        320        330        340        350
GDRPLHLASA KGFLNIAKLL MEEGSKADVN AQDNEDHVPL HFCSRFGHHD 
       360        370        380        390        400
IVKYLLQSDL EVQPHVVNIY GDTPLHLACY NGKFEVAKEI IQISGTESLT 
       410        420        430        440        450
KENIFSETAF HSACTYGKSI DLVKFLLDQN VININHQGRD GHTGLHSACY 
       460        470        480        490        500
HGHIRLVQFL LDNGADMNLV ACDPSRSSGE KDEQTCLMWA YEKGHDAIVT 
       510        520        530        540        550
LLKHYKRPQD ELPCNEYSQP GGDGSYVSVP SPLGKIKSMT KEKADILLLR 
       560        570        580        590        600
AGLPSHFHLQ LSEIEFHEII GSGSFGKVYK GRCRNKIVAI KRYRANTYCS 
       610        620        630        640        650
KSDVDMFCRE VSILCQLNHP CVIQFVGACL NDPSQFAIVT QYISGGSLFS 
       660        670        680        690        700
LLHEQKRILD LQSKLIIAVD VAKGMEYLHN LTQPIIHRDL NSHNILLYED 
       710        720        730        740        750
GHAVVADFGE SRFLQSLDED NMTKQPGNLR WMAPEVFTQC TRYTIKADVF 
       760        770        780        790        800
SYALCLWEIL TGEIPFAHLK PAAAAADMAY HHIRPPIGYS IPKPISSLLI 
       810        820        830        840        850
RGWNACPEGR PEFSEVVMKL EECLCNIELM SPASSNSSGS LSPSSSSDCL 
       860        870        880        890        900
VNRGGPGRSH VAALRSRFEL EYALNARSYA ALSQSAGQYS SQGLSLEEMK 
       910        920        930 
RSLQYTPIDK YGYVSDPMSS MHFHSCRNSS SFEDSS

Note: Based on a naturally occurring readthrough transcript which produces a FPGT-TNNI3K fusion protein.

Show »

Length:936

Mass (Da):104,179

Checksum:ⁱ081220549646863F

GO

Isoform 3Curated (identifier: Q59H18-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MGNYKSRPTQTCT → MAAARDPPEV...NSFTILLIHS
     591-596: SHNILL → RYFFPK
     597-835: Missing.

« Hide

        10         20         30         40         50
MAAARDPPEV SLREATQRKL RRFSELRGKL VARGEFWDIV AITAADEKQE 
        60         70         80         90        100
LAYNQQLSEK LKRKELPLGV QYHVFVDPAG AKIGNGGSTL CALQCLEKLY 
       110        120        130        140        150
GDKWNSFTIL LIHSDEWKKK VSESYVITIE RLEDDLQIKE KELTELRNIF 
       160        170        180        190        200
GSDEAFSKVN LNYRTENGLS LLHLCCICGG KKSHIRTLML KGLRPSRLTR 
       210        220        230        240        250
NGFTALHLAV YKDNAELITS LLHSGADIQQ VGYGGLTALH IATIAGHLEA 
       260        270        280        290        300
ADVLLQHGAN VNIQDAVFFT PLHIAAYYGH EQVTRLLLKF GADVNVSGEV 
       310        320        330        340        350
GDRPLHLASA KGFLNIAKLL MEEGSKADVN AQDNEDHVPL HFCSRFGHHD 
       360        370        380        390        400
IVKYLLQSDL EVQPHVVNIY GDTPLHLACY NGKFEVAKEI IQISGTESLT 
       410        420        430        440        450
KENIFSETAF HSACTYGKSI DLVKFLLDQN VININHQGRD GHTGLHSACY 
       460        470        480        490        500
HGHIRLVQFL LDNGADMNLV ACDPSRSSGE KDEQTCLMWA YEKGHDAIVT 
       510        520        530        540        550
LLKHYKRPQD ELPCNEYSQP GGDGSYVSVP SPLGKIKSMT KEKADILLLR 
       560        570        580        590        600
AGLPSHFHLQ LSEIEFHEII GSGSFGKVYK GRCRNKIVAI KRYRANTYCS 
       610        620        630        640        650
KSDVDMFCRE VSILCQLNHP CVIQFVGACL NDPSQFAIVT QYISGGSLFS 
       660        670        680        690 
LLHEQKRILD LQSKLIIAVD VAKGMEYLHN LTQPIIHRDL NRYFFPK

Note: Based on a naturally occurring readthrough transcript which produces a FPGT-TNNI3K fusion protein.

Show »

Length:697

Mass (Da):77,957

Checksum:ⁱ7567E27D45485C9C

GO

Isoform 4Curated (identifier: Q59H18-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MGNYKSRPTQTCT → MAAARDPPEV...NSFTILLIHS
     708-742: GRPEFSEVVMKLEECLCNIELMSPASSNSSGSLSP → AKSRPSHYPVSSVYTETLKKKNEDRFGMWIEYLRR
     743-835: Missing.

« Hide

        10         20         30         40         50
MAAARDPPEV SLREATQRKL RRFSELRGKL VARGEFWDIV AITAADEKQE 
        60         70         80         90        100
LAYNQQLSEK LKRKELPLGV QYHVFVDPAG AKIGNGGSTL CALQCLEKLY 
       110        120        130        140        150
GDKWNSFTIL LIHSDEWKKK VSESYVITIE RLEDDLQIKE KELTELRNIF 
       160        170        180        190        200
GSDEAFSKVN LNYRTENGLS LLHLCCICGG KKSHIRTLML KGLRPSRLTR 
       210        220        230        240        250
NGFTALHLAV YKDNAELITS LLHSGADIQQ VGYGGLTALH IATIAGHLEA 
       260        270        280        290        300
ADVLLQHGAN VNIQDAVFFT PLHIAAYYGH EQVTRLLLKF GADVNVSGEV 
       310        320        330        340        350
GDRPLHLASA KGFLNIAKLL MEEGSKADVN AQDNEDHVPL HFCSRFGHHD 
       360        370        380        390        400
IVKYLLQSDL EVQPHVVNIY GDTPLHLACY NGKFEVAKEI IQISGTESLT 
       410        420        430        440        450
KENIFSETAF HSACTYGKSI DLVKFLLDQN VININHQGRD GHTGLHSACY 
       460        470        480        490        500
HGHIRLVQFL LDNGADMNLV ACDPSRSSGE KDEQTCLMWA YEKGHDAIVT 
       510        520        530        540        550
LLKHYKRPQD ELPCNEYSQP GGDGSYVSVP SPLGKIKSMT KEKADILLLR 
       560        570        580        590        600
AGLPSHFHLQ LSEIEFHEII GSGSFGKVYK GRCRNKIVAI KRYRANTYCS 
       610        620        630        640        650
KSDVDMFCRE VSILCQLNHP CVIQFVGACL NDPSQFAIVT QYISGGSLFS 
       660        670        680        690        700
LLHEQKRILD LQSKLIIAVD VAKGMEYLHN LTQPIIHRDL NSHNILLYED 
       710        720        730        740        750
GHAVVADFGE SRFLQSLDED NMTKQPGNLR WMAPEVFTQC TRYTIKADVF 
       760        770        780        790        800
SYALCLWEIL TGEIPFAHLK PAAAAADMAY HHIRPPIGYS IPKPISSLLI 
       810        820        830        840 
RGWNACPEAK SRPSHYPVSS VYTETLKKKN EDRFGMWIEY LRR

Note: Based on a naturally occurring readthrough transcript which produces a FPGT-TNNI3K fusion protein.

Show »

Length:843

Mass (Da):94,517

Checksum:ⁱ3001399FAEAD7B24

GO

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

H0YDG1	H0YDG1_HUMAN	Serine/threonine-protein kinase TNN... Serine/threonine-protein kinase TNNI3K	TNNI3K	113	Annotation score:
H0YCE9	H0YCE9_HUMAN	Serine/threonine-protein kinase TNN... Serine/threonine-protein kinase TNNI3K	TNNI3K	150	Annotation score:
H0YE48	H0YE48_HUMAN	Serine/threonine-protein kinase TNN... Serine/threonine-protein kinase TNNI3K	TNNI3K	188	Annotation score:

Sequence cautionⁱ

The sequence BAD92178 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

The sequence CAE45949 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	127	I → V in AAH32865 (PubMed:15489334).Curated	1
Sequence conflictⁱ	250	I → M in CAE45949 (PubMed:17974005).Curated	1
Sequence conflictⁱ	367	N → S in CAE45949 (PubMed:17974005).Curated	1
Sequence conflictⁱ	629	R → L in AAH32865 (PubMed:15489334).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_041223	151	D → H1 PublicationCorresponds to variant dbSNP:rs34874695Ensembl.	1
Natural variantⁱVAR_041224	263	P → L1 PublicationCorresponds to variant dbSNP:rs34521608Ensembl.	1
Natural variantⁱVAR_041225	309	F → L1 Publication	1
Natural variantⁱVAR_041226	430	S → L in a colorectal adenocarcinoma sample; somatic mutation. 1 Publication	1
Natural variantⁱVAR_041227	510	V → L1 PublicationCorresponds to variant dbSNP:rs34335537Ensembl.	1
Natural variantⁱVAR_072650	526	G → D in CCDD; the mutation results in decreased protein solubility; causes abnormal aggregation; markedly reduced protein expression is observed in the sarcoplasm and nuclei of patient cardiomyocytes. 1 PublicationCorresponds to variant dbSNP:rs606231469EnsemblClinVar.	1
Natural variantⁱVAR_035639	629	R → G in a colorectal cancer sample; somatic mutation. 1 Publication	1
Natural variantⁱVAR_041228	637	T → M1 PublicationCorresponds to variant dbSNP:rs2274260Ensembl.	1
Natural variantⁱVAR_041229	686	I → T1 PublicationCorresponds to variant dbSNP:rs3737564Ensembl.	1
Natural variantⁱVAR_038821	785	A → G1 PublicationCorresponds to variant dbSNP:rs45578635Ensembl.	1
Natural variantⁱVAR_041230	798	M → I in a head & Neck squamous cell carcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs201613442Ensembl.	1
Natural variantⁱVAR_038822	833	D → Y1 PublicationCorresponds to variant dbSNP:rs45614933Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_039403	1 – 13	MGNYK…TQTCT → MAAARDPPEVSLREATQRKL RRFSELRGKLVARGEFWDIV AITAADEKQELAYNQQLSEK LKRKELPLGVQYHVFVDPAG AKIGNGGSTLCALQCLEKLY GDKWNSFTILLIHS in isoform 1, isoform 3 and isoform 4. 3 PublicationsAdd BLAST	13
Alternative sequenceⁱVSP_051882	591 – 596	SHNILL → RYFFPK in isoform 3. 1 Publication	6
Alternative sequenceⁱVSP_051883	597 – 835	Missing in isoform 3. 1 PublicationAdd BLAST	239
Alternative sequenceⁱVSP_051884	708 – 742	GRPEF…GSLSP → AKSRPSHYPVSSVYTETLKK KNEDRFGMWIEYLRR in isoform 4. 1 PublicationAdd BLAST	35
Alternative sequenceⁱVSP_051885	743 – 835	Missing in isoform 4. 1 PublicationAdd BLAST	93

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF116826 mRNA Translation: AAD29632.1 AY303691 mRNA Translation: AAP72030.1 AB208941 Transcribed RNA Translation: BAD92178.1 Different initiation. BX640903 mRNA Translation: CAE45949.1 Different initiation. DQ822519 Genomic DNA Translation: ABG46944.1 AC093158 Genomic DNA No translation available. AC098692 Genomic DNA No translation available. AC105271 Genomic DNA No translation available. AC119672 Genomic DNA No translation available. BX470253 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06415.1 BC032865 mRNA Translation: AAH32865.1 BC113539 mRNA Translation: AAI13540.1 BC117262 mRNA Translation: AAI17263.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS664.1 [Q59H18-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001186256.2, NM_001199327.1 NP_057062.1, NM_015978.2 [Q59H18-2]
UniGeneⁱ	Hs.480085

Genome annotation databases

Ensemblⁱ	ENST00000326637; ENSP00000322251; ENSG00000116783 [Q59H18-2]
GeneIDⁱ	100526835 51086
KEGGⁱ	hsa:100526835 hsa:51086
UCSC genome browser More...UCSCⁱ	uc001dgf.3 human [Q59H18-2]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q59H18	Serine/threonine-protein kinase TNNI3K		RAT		835	UniRef90_Q59H18
Serine/threonine-protein kinase TNNI3K		MOUSE		834
Uncharacterized protein		RABIT		986
Uncharacterized protein		MACMU		949
Uncharacterized protein		MACFA		949
+187
Q59H18-3	Serine/threonine-protein kinase TNNI3K		PONAB		618	UniRef90_Q59H18-3
Serine/threonine-protein kinase TNNI3K (Fragment)		PELCR		644
Serine/threonine-protein kinase TNNI3K (Fragment)		yellow-throated sandgrouse		621
Uncharacterized protein		PANPA		617
Uncharacterized protein		PANTR		617
+14

Protein	Similar proteins		Species	Score	Length	Source
Q59H18	Serine/threonine-protein kinase TNNI3K		RAT		835	UniRef50_Q59H18
Serine/threonine-protein kinase TNNI3K		MOUSE		834
Uncharacterized protein		RABIT		986
FPGT-TNNI3K isoform 1		PONAB		949
FPGT-TNNI3K readthrough		HUMAN		949
+273
Q59H18-3	Serine/threonine-protein kinase TNNI3K		PONAB		618	UniRef50_Q59H18-3
Serine/threonine-protein kinase TNNI3K (Fragment)		PELCR		644
Serine/threonine-protein kinase TNNI3K (Fragment)		yellow-throated sandgrouse		621
Uncharacterized protein		PANPA		617
Uncharacterized protein		PANTR		617
+20

Cross-referencesⁱ

Web resourcesⁱ

SeattleSNPs

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF116826 mRNA Translation: AAD29632.1 AY303691 mRNA Translation: AAP72030.1 AB208941 Transcribed RNA Translation: BAD92178.1 Different initiation. BX640903 mRNA Translation: CAE45949.1 Different initiation. DQ822519 Genomic DNA Translation: ABG46944.1 AC093158 Genomic DNA No translation available. AC098692 Genomic DNA No translation available. AC105271 Genomic DNA No translation available. AC119672 Genomic DNA No translation available. BX470253 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06415.1 BC032865 mRNA Translation: AAH32865.1 BC113539 mRNA Translation: AAI13540.1 BC117262 mRNA Translation: AAI17263.1
CCDSⁱ	CCDS664.1 [Q59H18-2]
RefSeqⁱ	NP_001186256.2, NM_001199327.1 NP_057062.1, NM_015978.2 [Q59H18-2]
UniGeneⁱ	Hs.480085

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4YFF	X-ray	3.07	A/B/C/D	420-730	[»]
	4YFI	X-ray	2.70	A/B/C/D	402-730	[»]
	6B5J	X-ray	2.97	A/B/C/D	420-730	[»]
ProteinModelPortalⁱ	Q59H18
SMRⁱ	Q59H18
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	119276, 11 interactors
IntActⁱ	Q59H18, 8 interactors
STRINGⁱ	9606.ENSP00000450895

Chemistry databases

BindingDBⁱ	Q59H18
ChEMBLⁱ	CHEMBL5260

PTM databases

iPTMnetⁱ	Q59H18
PhosphoSitePlusⁱ	Q59H18

Polymorphism and mutation databases

BioMutaⁱ	TNNI3K
DMDMⁱ	300669705

Proteomic databases

PaxDbⁱ	Q59H18
PeptideAtlasⁱ	Q59H18
PRIDEⁱ	Q59H18
ProteomicsDBⁱ	62665 62666 [Q59H18-1] 62667 [Q59H18-3] 62668 [Q59H18-4]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	51086
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000326637; ENSP00000322251; ENSG00000116783 [Q59H18-2]
GeneIDⁱ	100526835 51086
KEGGⁱ	hsa:100526835 hsa:51086
UCSCⁱ	uc001dgf.3 human [Q59H18-2]

Organism-specific databases

CTDⁱ	100526835 51086
DisGeNETⁱ	51086
EuPathDBⁱ	HostDB:ENSG00000116783.14
GeneCardsⁱ	TNNI3K
HGNCⁱ	HGNC:19661 TNNI3K
HPAⁱ	HPA072331
MalaCardsⁱ	TNNI3K
MIMⁱ	613932 gene 616117 phenotype
neXtProtⁱ	NX_Q59H18
OpenTargetsⁱ	ENSG00000116783 ENSG00000259030
PharmGKBⁱ	PA134976654
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IT5N Eukaryota COG0666 LUCA
GeneTreeⁱ	ENSGT00900000140790
HOGENOMⁱ	HOG000234421
HOVERGENⁱ	HBG079513
InParanoidⁱ	Q59H18
KOⁱ	K17535
OrthoDBⁱ	EOG091G01QL
PhylomeDBⁱ	Q59H18

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q59H18
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000116783 Expressed in 88 organ(s), highest expression level in heart
CleanExⁱ	HS_TNNI3K
ExpressionAtlasⁱ	Q59H18 baseline and differential
Genevisibleⁱ	Q59H18 HS

Family and domain databases

CDDⁱ	cd00204 ANK, 5 hits
Gene3Dⁱ	1.25.40.20, 4 hits
InterProⁱ	View protein in InterPro IPR002110 Ankyrin_rpt IPR020683 Ankyrin_rpt-contain_dom IPR036770 Ankyrin_rpt-contain_sf IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom
Pfamⁱ	View protein in Pfam PF12796 Ank_2, 3 hits PF07714 Pkinase_Tyr, 1 hit
PRINTSⁱ	PR01415 ANKYRIN
SMARTⁱ	View protein in SMART SM00248 ANK, 10 hits
SUPFAMⁱ	SSF48403 SSF48403, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS50297 ANK_REP_REGION, 1 hit PS50088 ANK_REPEAT, 6 hits PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TNI3K_HUMAN
Accessionⁱ	Q59H18Primary (citable) accession number: Q59H18 Secondary accession number(s): Q17RN0 , Q49AR1, Q6MZS9, Q9Y2V6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
	Last sequence update:	July 13, 2010
	Last modified:	October 10, 2018
	This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human and mouse protein kinases
Human and mouse protein kinases: classification and index
SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q59H18 (TNI3K_HUMAN)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Serine/threonine-protein kinase TNNI3K

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Keywords - Diseasei

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Coiled coil

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ