UniProtKB - Q59560 (RECA_MYCS2)
Protein
Protein RecA
Gene
recA
Organism
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Status
Functioni
Required for homologous recombination (HR) and the bypass of mutagenic DNA lesions (double strand breaks, DSB) by the SOS response. Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. Numerous X-ray crystals have been resolved under different conditions which indicate the flexibility of the protein, essential to its function. Gln-196 contributes to this plasticity by acting as a switch residue, which transmits the effect of nucleotide binding to the DNA-binding region.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 196 | Phosphate1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 71 – 76 | ATP2 Publications | 6 | |
Nucleotide bindingi | 102 – 105 | ATP2 Publications | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- damaged DNA binding Source: UniProtKB-UniRule
- DNA-dependent ATPase activity Source: UniProtKB-UniRule
- single-stranded DNA binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA recombination Source: UniProtKB-UniRule
- DNA repair Source: UniProtKB-UniRule
- SOS response Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA recombination, DNA repair, SOS response |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MSME246196:G1H7P-2722-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Protein RecAUniRule annotationAlternative name(s): Recombinase AUniRule annotation |
Gene namesi | Name:recAUniRule annotation Ordered Locus Names:MSMEG_2723, MSMEI_2656 |
Organismi | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
Taxonomic identifieri | 246196 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycolicibacterium › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Not essential in the absence of DNA damage, its deletion renders cells much more sensitive to DNA damaging agents such as UV light, ionizing radiation, alkylating agents and DNA gyrase inhibitors and desiccation-induced DSBs. 5-fold decrease in survival, loss of HR, no change in NHEJ (non-homologous end-joining) or single-strand annealing DSB repair.3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 196 | Q → A, E or N: Loss of residue movement, loss of switch function in crystal structures. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB02930, Adenosine 5'-[gamma-thio]triphosphate DB03222, dATP |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122767 | 1 – 349 | Protein RecAAdd BLAST | 349 |
Proteomic databases
PRIDEi | Q59560 |
Interactioni
Subunit structurei
Polymerizes non-specifically on ssDNA to form filaments.
Interacts with and activates LexA leading to autocatalytic cleavage of LexA, which derepresses the SOS regulon and activates DNA repair (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 246196.MSMEI_2656 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q59560 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q59560 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 71 – 75 | Phosphate binding1 Publication | 5 |
Sequence similaritiesi
Belongs to the RecA family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0468, Bacteria |
OMAi | DYGEQAL |
OrthoDBi | 1080436at2 |
Family and domain databases
CDDi | cd00983, recA, 1 hit |
Gene3Di | 3.30.250.10, 1 hit |
HAMAPi | MF_00268, RecA, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR013765, DNA_recomb/repair_RecA IPR020584, DNA_recomb/repair_RecA_CS IPR027417, P-loop_NTPase IPR020588, RecA_ATP-bd IPR023400, RecA_C IPR020587, RecA_monomer-monomer_interface |
PANTHERi | PTHR45900, PTHR45900, 1 hit |
Pfami | View protein in Pfam PF00154, RecA, 1 hit |
PRINTSi | PR00142, RECA |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF54752, SSF54752, 1 hit |
TIGRFAMsi | TIGR02012, tigrfam_recA, 1 hit |
PROSITEi | View protein in PROSITE PS00321, RECA_1, 1 hit PS50162, RECA_2, 1 hit PS50163, RECA_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q59560-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQQAPDREK ALELAMAQID KNFGKGSVMR LGEEVRQPIS VIPTGSISLD
60 70 80 90 100
VALGIGGLPR GRVIEIYGPE SSGKTTVALH AVANAQAAGG IAAFIDAEHA
110 120 130 140 150
LDPEYAKKLG VDTDSLLVSQ PDTGEQALEI ADMLVRSGAL DIIVIDSVAA
160 170 180 190 200
LVPRAEIEGE MGDSHVGLQA RLMSQALRKM TGALNNSGTT AIFINQLREK
210 220 230 240 250
IGVMFGSPET TTGGKALKFY ASVRLDVRRI ETLKDGTDAV GNRTRVKVVK
260 270 280 290 300
NKVSPPFKQA EFDILYGQGI SREGSLIDMG VEHGFIRKSG SWFTYEGEQL
310 320 330 340
GQGKENARKF LLENTDVANE IEKKIKEKLG IGAVVTAEAD DVLPAPVDF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X99208 Genomic DNA Translation: CAA67597.1 CP000480 Genomic DNA Translation: ABK74334.1 CP001663 Genomic DNA Translation: AFP39124.1 |
RefSeqi | WP_003894107.1, NZ_SIJM01000032.1 YP_887057.1, NC_008596.1 |
Genome annotation databases
EnsemblBacteriai | ABK74334; ABK74334; MSMEG_2723 AFP39124; AFP39124; MSMEI_2656 |
GeneIDi | 45744261 |
KEGGi | msg:MSMEI_2656 msm:MSMEG_2723 |
PATRICi | fig|246196.19.peg.2690 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X99208 Genomic DNA Translation: CAA67597.1 CP000480 Genomic DNA Translation: ABK74334.1 CP001663 Genomic DNA Translation: AFP39124.1 |
RefSeqi | WP_003894107.1, NZ_SIJM01000032.1 YP_887057.1, NC_008596.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UBC | X-ray | 3.80 | A | 1-349 | [»] | |
1UBE | X-ray | 3.30 | A | 1-349 | [»] | |
1UBF | X-ray | 3.50 | A | 1-349 | [»] | |
1UBG | X-ray | 3.50 | A | 1-349 | [»] | |
2G88 | X-ray | 3.20 | A | 1-349 | [»] | |
2ODN | X-ray | 3.10 | A | 1-349 | [»] | |
2ODW | X-ray | 3.30 | A | 1-349 | [»] | |
2OE2 | X-ray | 3.45 | A | 1-349 | [»] | |
2OEP | X-ray | 3.10 | A | 1-349 | [»] | |
2OES | X-ray | 3.50 | A | 1-349 | [»] | |
2OFO | X-ray | 3.16 | A | 1-349 | [»] | |
2ZR0 | X-ray | 3.00 | A | 1-349 | [»] | |
2ZR7 | X-ray | 3.60 | A | 1-349 | [»] | |
2ZR9 | X-ray | 2.50 | A | 1-349 | [»] | |
2ZRA | X-ray | 3.10 | A | 1-349 | [»] | |
2ZRB | X-ray | 3.25 | A | 1-349 | [»] | |
2ZRC | X-ray | 3.10 | A | 1-349 | [»] | |
2ZRD | X-ray | 3.10 | A | 1-349 | [»] | |
2ZRE | X-ray | 2.90 | A | 1-349 | [»] | |
2ZRF | X-ray | 3.00 | A | 1-349 | [»] | |
2ZRG | X-ray | 3.50 | A | 1-349 | [»] | |
2ZRH | X-ray | 3.20 | A | 1-349 | [»] | |
2ZRI | X-ray | 3.30 | A | 1-349 | [»] | |
2ZRJ | X-ray | 2.60 | A | 1-349 | [»] | |
2ZRK | X-ray | 3.20 | A | 1-349 | [»] | |
2ZRL | X-ray | 3.70 | A | 1-349 | [»] | |
2ZRM | X-ray | 2.80 | A | 1-349 | [»] | |
2ZRN | X-ray | 3.30 | A | 1-349 | [»] | |
2ZRO | X-ray | 2.90 | A | 1-349 | [»] | |
2ZRP | X-ray | 3.30 | A | 1-349 | [»] | |
SMRi | Q59560 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 246196.MSMEI_2656 |
Chemistry databases
DrugBanki | DB02930, Adenosine 5'-[gamma-thio]triphosphate DB03222, dATP |
Proteomic databases
PRIDEi | Q59560 |
Genome annotation databases
EnsemblBacteriai | ABK74334; ABK74334; MSMEG_2723 AFP39124; AFP39124; MSMEI_2656 |
GeneIDi | 45744261 |
KEGGi | msg:MSMEI_2656 msm:MSMEG_2723 |
PATRICi | fig|246196.19.peg.2690 |
Phylogenomic databases
eggNOGi | COG0468, Bacteria |
OMAi | DYGEQAL |
OrthoDBi | 1080436at2 |
Enzyme and pathway databases
BioCyci | MSME246196:G1H7P-2722-MONOMER |
Miscellaneous databases
EvolutionaryTracei | Q59560 |
Family and domain databases
CDDi | cd00983, recA, 1 hit |
Gene3Di | 3.30.250.10, 1 hit |
HAMAPi | MF_00268, RecA, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR013765, DNA_recomb/repair_RecA IPR020584, DNA_recomb/repair_RecA_CS IPR027417, P-loop_NTPase IPR020588, RecA_ATP-bd IPR023400, RecA_C IPR020587, RecA_monomer-monomer_interface |
PANTHERi | PTHR45900, PTHR45900, 1 hit |
Pfami | View protein in Pfam PF00154, RecA, 1 hit |
PRINTSi | PR00142, RECA |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF54752, SSF54752, 1 hit |
TIGRFAMsi | TIGR02012, tigrfam_recA, 1 hit |
PROSITEi | View protein in PROSITE PS00321, RECA_1, 1 hit PS50162, RECA_2, 1 hit PS50163, RECA_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RECA_MYCS2 | |
Accessioni | Q59560Primary (citable) accession number: Q59560 Secondary accession number(s): A0QVW9, I7G989 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families