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Protein

Chondroitinase-AC

Gene

cslA

Organism
Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups.

Cofactori

Ca2+Note: Binds 1 Ca2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2251
Active sitei2341
Active sitei2881
Metal bindingi405Calcium1
Metal bindingi407Calcium1
Metal bindingi416Calcium1
Metal bindingi417Calcium; via carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15799
PHEP485917:G1GFH-801-MONOMER
BRENDAi4.2.2.5 2286

Protein family/group databases

CAZyiPL8 Polysaccharide Lyase Family 8

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitinase-AC (EC:4.2.2.5)
Alternative name(s):
Chondroitin sulfate AC lyase
Chondroitin-AC eliminase
Chondroitin-AC lyase
Gene namesi
Name:cslA
Synonyms:chnAC
Ordered Locus Names:Phep_0786
OrganismiPedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3)
Taxonomic identifieri485917 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesSphingobacteriiaSphingobacterialesSphingobacteriaceaePedobacter
Proteomesi
  • UP000000852 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Chemistry databases

DrugBankiDB03863 2-O-Methyl Fucose
DB01872 Acetylgalactosamine-4-Sulfate
DB03389 alpha-D-Xylopyranose
DB02945 alpha-L-iduronic acid
DB03879 alpha-L-methyl-fucose
DB03156 D-Glucuronic Acid
DB02186 N-Acetyl-D-Galactosamine 6-Sulfate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000002492723 – 700Chondroitinase-ACAdd BLAST678

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi328O-linked (Man...) serine1
Glycosylationi455O-linked (Man...) serine1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ59288

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi485917.Phep_0786

Structurei

Secondary structure

1700
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ59288
SMRiQ59288
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59288

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide lyase 8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4106AM8 Bacteria
ENOG410XTFC LUCA
HOGENOMiHOG000105747
KOiK19049
OMAiRGDEYHN
OrthoDBiPOG091H03FL

Family and domain databases

Gene3Di1.50.10.100, 1 hit
2.60.220.10, 1 hit
2.70.98.10, 1 hit
InterProiView protein in InterPro
IPR008929 Chondroitin_lyas
IPR011013 Gal_mutarotase_sf_dom
IPR014718 GH-type_carb-bd
IPR038970 Lyase_8
IPR011071 Lyase_8-like_C
IPR012970 Lyase_8_alpha_N
IPR004103 Lyase_8_C
IPR003159 Lyase_8_central_dom
PANTHERiPTHR38481 PTHR38481, 1 hit
PfamiView protein in Pfam
PF02278 Lyase_8, 1 hit
PF02884 Lyase_8_C, 1 hit
PF08124 Lyase_8_N, 1 hit
SUPFAMiSSF48230 SSF48230, 1 hit
SSF49863 SSF49863, 1 hit
SSF74650 SSF74650, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q59288-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKLFVTCIV FFSILSPALL IAQQTGTAEL IMKRVMLDLK KPLRNMDKVA
60 70 80 90 100
EKNLNTLQPD GSWKDVPYKD DAMTNWLPNN HLLQLETIIQ AYIEKDSHYY
110 120 130 140 150
GDDKVFDQIS KAFKYWYDSD PKSRNWWHNE IATPQALGEM LILMRYGKKP
160 170 180 190 200
LDEALVHKLT ERMKRGEPEK KTGANKTDIA LHYFYRALLT SDEALLSFAV
210 220 230 240 250
KELFYPVQFV HYEEGLQYDY SYLQHGPQLQ ISSYGAVFIT GVLKLANYVR
260 270 280 290 300
DTPYALSTEK LAIFSKYYRD SYLKAIRGSY MDFNVEGRGV SRPDILNKKA
310 320 330 340 350
EKKRLLVAKM IDLKHTEEWA DAIARTDSTV AAGYKIEPYH HQFWNGDYVQ
360 370 380 390 400
HLRPAYSFNV RMVSKRTRRS ESGNKENLLG RYLSDGATNI QLRGPEYYNI
410 420 430 440 450
MPVWEWDKIP GITSRDYLTD RPLTKLWGEQ GSNDFAGGVS DGVYGASAYA
460 470 480 490 500
LDYDSLQAKK AWFFFDKEIV CLGAGINSNA PENITTTLNQ SWLNGPVIST
510 520 530 540 550
AGKTGRGKIT TFKAQGQFWL LHDAIGYYFP EGANLSLSTQ SQKGNWFHIN
560 570 580 590 600
NSHSKDEVSG DVFKLWINHG ARPENAQYAY IVLPGINKPE EIKKYNGTAP
610 620 630 640 650
KVLANTNQLQ AVYHQQLDMV QAIFYTAGKL SVAGIEIETD KPCAVLIKHI
660 670 680 690 700
NGKQVIWAAD PLQKEKTAVL SIRDLKTGKT NRVKIDFPQQ EFAGATVELK
Length:700
Mass (Da):79,694
Last modified:November 1, 1996 - v1
Checksum:iC36B608FCAFFC656
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27583 Genomic DNA Translation: AAC83383.1
CP001681 Genomic DNA Translation: ACU03008.1
RefSeqiWP_012780954.1, NZ_AQGK01000003.1

Genome annotation databases

EnsemblBacteriaiACU03008; ACU03008; Phep_0786
KEGGiphe:Phep_0786

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27583 Genomic DNA Translation: AAC83383.1
CP001681 Genomic DNA Translation: ACU03008.1
RefSeqiWP_012780954.1, NZ_AQGK01000003.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CB8X-ray1.90A23-700[»]
1HM2X-ray2.00A1-700[»]
1HM3X-ray2.10A1-700[»]
1HMUX-ray2.00A1-700[»]
1HMWX-ray2.30A1-700[»]
ProteinModelPortaliQ59288
SMRiQ59288
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi485917.Phep_0786

Chemistry databases

DrugBankiDB03863 2-O-Methyl Fucose
DB01872 Acetylgalactosamine-4-Sulfate
DB03389 alpha-D-Xylopyranose
DB02945 alpha-L-iduronic acid
DB03879 alpha-L-methyl-fucose
DB03156 D-Glucuronic Acid
DB02186 N-Acetyl-D-Galactosamine 6-Sulfate

Protein family/group databases

CAZyiPL8 Polysaccharide Lyase Family 8

Proteomic databases

PRIDEiQ59288

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACU03008; ACU03008; Phep_0786
KEGGiphe:Phep_0786

Phylogenomic databases

eggNOGiENOG4106AM8 Bacteria
ENOG410XTFC LUCA
HOGENOMiHOG000105747
KOiK19049
OMAiRGDEYHN
OrthoDBiPOG091H03FL

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15799
PHEP485917:G1GFH-801-MONOMER
BRENDAi4.2.2.5 2286

Miscellaneous databases

EvolutionaryTraceiQ59288

Family and domain databases

Gene3Di1.50.10.100, 1 hit
2.60.220.10, 1 hit
2.70.98.10, 1 hit
InterProiView protein in InterPro
IPR008929 Chondroitin_lyas
IPR011013 Gal_mutarotase_sf_dom
IPR014718 GH-type_carb-bd
IPR038970 Lyase_8
IPR011071 Lyase_8-like_C
IPR012970 Lyase_8_alpha_N
IPR004103 Lyase_8_C
IPR003159 Lyase_8_central_dom
PANTHERiPTHR38481 PTHR38481, 1 hit
PfamiView protein in Pfam
PF02278 Lyase_8, 1 hit
PF02884 Lyase_8_C, 1 hit
PF08124 Lyase_8_N, 1 hit
SUPFAMiSSF48230 SSF48230, 1 hit
SSF49863 SSF49863, 1 hit
SSF74650 SSF74650, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCSLA_PEDHD
AccessioniPrimary (citable) accession number: Q59288
Secondary accession number(s): C6Y215
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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