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Protein

3-carboxy-cis,cis-muconate cycloisomerase

Gene

pcaB

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes an anti cycloisomerization.

Catalytic activityi

2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate.

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from 3-carboxy-cis,cis-muconate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-carboxy-cis,cis-muconate cycloisomerase (pcaB)
  2. 4-carboxymuconolactone decarboxylase (pcaC)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from 3-carboxy-cis,cis-muconate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAromatic hydrocarbons catabolism

Enzyme and pathway databases

BioCyciASP62977:ACIAD_RS07865-MONOMER
UniPathwayiUPA00157; UER00265

Names & Taxonomyi

Protein namesi
Recommended name:
3-carboxy-cis,cis-muconate cycloisomerase (EC:5.5.1.2)
Alternative name(s):
3-carboxymuconate lactonizing enzyme
Short name:
CMLE
Gene namesi
Name:pcaB
Ordered Locus Names:ACIAD1707
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
Proteomesi
  • UP000000430 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001613471 – 4513-carboxy-cis,cis-muconate cycloisomeraseAdd BLAST451

Proteomic databases

PRIDEiQ59092

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi62977.ACIAD1707

Structurei

Secondary structure

1451
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 9Combined sources4
Helixi12 – 17Combined sources6
Helixi20 – 40Combined sources21
Helixi46 – 56Combined sources11
Helixi59 – 62Combined sources4
Helixi65 – 75Combined sources11
Beta strandi76 – 78Combined sources3
Helixi79 – 91Combined sources13
Helixi97 – 100Combined sources4
Turni101 – 104Combined sources4
Helixi107 – 143Combined sources37
Turni144 – 146Combined sources3
Beta strandi148 – 153Combined sources6
Beta strandi156 – 162Combined sources7
Helixi163 – 188Combined sources26
Helixi201 – 203Combined sources3
Helixi207 – 218Combined sources12
Helixi232 – 258Combined sources27
Turni261 – 263Combined sources3
Helixi287 – 308Combined sources22
Helixi320 – 348Combined sources29
Helixi353 – 361Combined sources9
Turni362 – 365Combined sources4
Helixi366 – 368Combined sources3
Helixi369 – 380Combined sources12
Helixi382 – 399Combined sources18
Helixi403 – 407Combined sources5
Helixi411 – 414Combined sources4
Helixi419 – 425Combined sources7
Helixi428 – 431Combined sources4
Helixi435 – 444Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q5NX-ray2.30A1-451[»]
ProteinModelPortaliQ59092
SMRiQ59092
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ59092

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family.Curated

Phylogenomic databases

eggNOGiENOG4107RAW Bacteria
COG0015 LUCA
HOGENOMiHOG000033914
KOiK01857
OMAiHRATPMI
OrthoDBiPOG091H0168

Family and domain databases

Gene3Di1.10.275.10, 1 hit
InterProiView protein in InterPro
IPR019468 AdenyloSucc_lyase_C
IPR024083 Fumarase/histidase_N
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
IPR012789 Protocat_PcaB
PfamiView protein in Pfam
PF10397 ADSL_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SMARTiView protein in SMART
SM00998 ADSL_C, 1 hit
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR02426 protocat_pcaB, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q59092-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQLYASLFY QRDVTEIFSD RALVSYMVEA EVALAQAQAQ VGVIPQSAAT
60 70 80 90 100
VIERAAKTAI DKIDFDALAT ATGLAGNIAI PFVKQLTAIV KDADEDAARY
110 120 130 140 150
VHWGATSQDI LDTACILQCR DALAIVQNQV QQCYETALSQ AQTYRHQVMM
160 170 180 190 200
GRTWLQQALP ITLGHKLARW ASAFKRDLDR INAIKARVLV AQLGGAVGSL
210 220 230 240 250
ASLQDQGSIV VEAYAKQLKL GQTACTWHGE RDRIVEIASV LGIITGNVGK
260 270 280 290 300
MARDWSLMMQ TEIAEVFEPT AKGRGGSSTM PHKRNPVAAA SVLAAANRVP
310 320 330 340 350
ALMSSIYQSM VQEHERSLGA WHAEWLSLPE IFQLTAGALE RTLDVLKGME
360 370 380 390 400
VNAENMHQNI ECTHGLIMAE AVMMALAPHM GRLNAHHVVE AACKTAVAEQ
410 420 430 440 450
KHLKDIISQV DEVKQYFNPS QLDEIFKPES YLGNIQDQID AVLQEAKGEA

K
Length:451
Mass (Da):49,316
Last modified:August 31, 2004 - v2
Checksum:i831FE94AC6574617
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37A → Q in AAC37149 (PubMed:8063101).Curated1
Sequence conflicti50 – 56TVIERAA → NCDRTCQQ in AAC37149 (PubMed:8063101).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05770 Genomic DNA Translation: AAC37149.1
CR543861 Genomic DNA Translation: CAG68549.1
RefSeqiWP_004926621.1, NC_005966.1

Genome annotation databases

EnsemblBacteriaiCAG68549; CAG68549; ACIAD1707
KEGGiaci:ACIAD1707

Similar proteinsi

Entry informationi

Entry nameiPCAB_ACIAD
AccessioniPrimary (citable) accession number: Q59092
Secondary accession number(s): Q6FBL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 31, 2004
Last modified: June 20, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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