We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - Q58HT5 (AWAT1_HUMAN)
Protein
Acyl-CoA wax alcohol acyltransferase 1
Gene
AWAT1
Organism
Homo sapiens (Human)
Status
Functioni
Acyltransferase that catalyzes the formation of ester bonds between fatty alcohols and fatty acyl-CoAs to form wax monoesters (PubMed:15671038).
Shows a strong preference for decyl alcohol (C10), with less activity towards C16 and C18 alcohols (PubMed:15671038).
Shows a strong preference for saturated acyl-CoAs (PubMed:15671038).
1 PublicationCatalytic activityi
- EC:2.3.1.751 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- (9Z)-hexadecen-1-ol + (9Z)-octadecenoyl-CoA = 1-O-(9Z)-hexadecenyl (9Z)-octadecenoate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecen-1-ol + (9Z)-octadecenoyl-CoA = 1-O-(9Z)-octadecenyl (9Z)-octadecenoate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
GO - Molecular functioni
- arachidoyl-CoA:1-dodecanol O-acyltransferase activity Source: UniProtKB-EC
- long-chain-alcohol O-fatty-acyltransferase activity Source: Reactome
GO - Biological processi
- arachidonic acid metabolic process Source: Reactome
- lipid metabolic process Source: GO_Central
- wax biosynthetic process Source: Reactome
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.75, 2681 |
PathwayCommonsi | Q58HT5 |
Reactomei | R-HSA-2142753, Arachidonic acid metabolism R-HSA-9640463, Wax biosynthesis |
Chemistry databases
SwissLipidsi | SLP:000000306 |
Names & Taxonomyi
Protein namesi | Recommended name: Acyl-CoA wax alcohol acyltransferase 1 (EC:2.3.1.751 Publication)Alternative name(s): Diacylglycerol O-acyltransferase 2-like protein 3 Diacylglycerol acyltransferase 2 Long-chain-alcohol O-fatty-acyltransferase 1 |
Gene namesi | Name:AWAT1 Synonyms:DGA2, DGAT2L3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:23252, AWAT1 |
MIMi | 300924, gene |
neXtProti | NX_Q58HT5 |
VEuPathDBi | HostDB:ENSG00000204195 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 12 – 32 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 34 – 53 | HelicalSequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 158833 |
OpenTargetsi | ENSG00000204195 |
PharmGKBi | PA164716409 |
Miscellaneous databases
Pharosi | Q58HT5, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2375205 |
Genetic variation databases
BioMutai | AWAT1 |
DMDMi | 74741070 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249051 | 1 – 328 | Acyl-CoA wax alcohol acyltransferase 1Add BLAST | 328 |
Proteomic databases
PaxDbi | Q58HT5 |
PeptideAtlasi | Q58HT5 |
PRIDEi | Q58HT5 |
Expressioni
Tissue specificityi
Predominantly expressed in skin, where it is limited to the sebaceous gland. Expressed in more mature, centrally located cells just before their rupture and sebum release. Also expressed in all tissues except spleen. Expressed at higher level in thymus, prostate and testis.1 Publication
Gene expression databases
Bgeei | ENSG00000204195, Expressed in mammalian vulva and 42 other tissues |
Genevisiblei | Q58HT5, HS |
Organism-specific databases
HPAi | ENSG00000204195, Tissue enhanced (skin) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 127715, 3 interactors |
STRINGi | 9606.ENSP00000363645 |
Chemistry databases
BindingDBi | Q58HT5 |
Miscellaneous databases
RNActi | Q58HT5, protein |
Structurei
3D structure databases
AlphaFoldDBi | Q58HT5 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Sequence similaritiesi
Belongs to the diacylglycerol acyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT01030000234582 |
HOGENOMi | CLU_023995_0_0_1 |
InParanoidi | Q58HT5 |
OMAi | FYCCVFY |
OrthoDBi | 1347007at2759 |
PhylomeDBi | Q58HT5 |
TreeFami | TF314707 |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q58HT5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAHSKQPSHF QSLMLLQWPL SYLAIFWILQ PLFVYLLFTS LWPLPVLYFA
60 70 80 90 100
WLFLDWKTPE RGGRRSAWVR NWCVWTHIRD YFPITILKTK DLSPEHNYLM
110 120 130 140 150
GVHPHGLLTF GAFCNFCTEA TGFSKTFPGI TPHLATLSWF FKIPFVREYL
160 170 180 190 200
MAKGVCSVSQ PAINYLLSHG TGNLVGIVVG GVGEALQSVP NTTTLILQKR
210 220 230 240 250
KGFVRTALQH GAHLVPTFTF GETEVYDQVL FHKDSRMYKF QSCFRRIFGF
260 270 280 290 300
YCCVFYGQSF CQGSTGLLPY SRPIVTVVGE PLPLPQIEKP SQEMVDKYHA
310 320
LYMDALHKLF DQHKTHYGCS ETQKLFFL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY947638 mRNA Translation: AAX48018.1 AL357752 Genomic DNA No translation available. BC039181 Genomic DNA No translation available. BN000155 mRNA Translation: CAD89266.1 |
CCDSi | CCDS35321.1 |
RefSeqi | NP_001013597.1, NM_001013579.2 |
Genome annotation databases
Ensembli | ENST00000374521.4; ENSP00000363645.3; ENSG00000204195.4 |
GeneIDi | 158833 |
KEGGi | hsa:158833 |
MANE-Selecti | ENST00000374521.4; ENSP00000363645.3; NM_001013579.3; NP_001013597.1 |
UCSCi | uc004dxy.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY947638 mRNA Translation: AAX48018.1 AL357752 Genomic DNA No translation available. BC039181 Genomic DNA No translation available. BN000155 mRNA Translation: CAD89266.1 |
CCDSi | CCDS35321.1 |
RefSeqi | NP_001013597.1, NM_001013579.2 |
3D structure databases
AlphaFoldDBi | Q58HT5 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 127715, 3 interactors |
STRINGi | 9606.ENSP00000363645 |
Chemistry databases
BindingDBi | Q58HT5 |
ChEMBLi | CHEMBL2375205 |
SwissLipidsi | SLP:000000306 |
Genetic variation databases
BioMutai | AWAT1 |
DMDMi | 74741070 |
Proteomic databases
PaxDbi | Q58HT5 |
PeptideAtlasi | Q58HT5 |
PRIDEi | Q58HT5 |
Protocols and materials databases
Antibodypediai | 27356, 100 antibodies from 20 providers |
DNASUi | 158833 |
Genome annotation databases
Ensembli | ENST00000374521.4; ENSP00000363645.3; ENSG00000204195.4 |
GeneIDi | 158833 |
KEGGi | hsa:158833 |
MANE-Selecti | ENST00000374521.4; ENSP00000363645.3; NM_001013579.3; NP_001013597.1 |
UCSCi | uc004dxy.4, human |
Organism-specific databases
CTDi | 158833 |
DisGeNETi | 158833 |
GeneCardsi | AWAT1 |
HGNCi | HGNC:23252, AWAT1 |
HPAi | ENSG00000204195, Tissue enhanced (skin) |
MIMi | 300924, gene |
neXtProti | NX_Q58HT5 |
OpenTargetsi | ENSG00000204195 |
PharmGKBi | PA164716409 |
VEuPathDBi | HostDB:ENSG00000204195 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT01030000234582 |
HOGENOMi | CLU_023995_0_0_1 |
InParanoidi | Q58HT5 |
OMAi | FYCCVFY |
OrthoDBi | 1347007at2759 |
PhylomeDBi | Q58HT5 |
TreeFami | TF314707 |
Enzyme and pathway databases
BRENDAi | 2.3.1.75, 2681 |
PathwayCommonsi | Q58HT5 |
Reactomei | R-HSA-2142753, Arachidonic acid metabolism R-HSA-9640463, Wax biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 158833, 11 hits in 691 CRISPR screens |
GenomeRNAii | 158833 |
Pharosi | Q58HT5, Tbio |
PROi | PR:Q58HT5 |
RNActi | Q58HT5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204195, Expressed in mammalian vulva and 42 other tissues |
Genevisiblei | Q58HT5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AWAT1_HUMAN | |
Accessioni | Q58HT5Primary (citable) accession number: Q58HT5 Secondary accession number(s): Q5JT21, Q6IEE4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | April 26, 2005 | |
Last modified: | May 25, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families