UniProtKB - Q58DD0 (ACE2_BOVIN)
Angiotensin-converting enzyme 2
ACE2
Functioni
Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins, casomorphins and dynorphin A. Plays an important role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 in intestine, regulating trafficking, expression on the cell surface, and its catalytic activity.
By similarityCatalytic activityi
- EC:3.4.17.23By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Cofactori
Protein has several cofactor binding sites:- Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
- chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 168 | ChlorideBy similarity | 1 | |
Binding sitei | 272 | SubstrateBy similarity | 1 | |
Metal bindingi | 373 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 374 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 377 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 401 | Zinc; catalyticBy similarity | 1 | |
Binding sitei | 476 | ChlorideBy similarity | 1 | |
Binding sitei | 480 | ChlorideBy similarity | 1 | |
Active sitei | 504 | Proton donorBy similarity | 1 | |
Binding sitei | 514 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carboxypeptidase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- metallopeptidase activity Source: GO_Central
- peptidyl-dipeptidase activity Source: InterPro
- virus receptor activity Source: UniProtKB
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Chloride, Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M02.006 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ACE2 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
- Apical cell membrane By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cilium By similarity
Note: Detected in both cell membrane and cytoplasm in neurons.By similarity
Extracellular region or secreted
- extracellular space Source: GO_Central
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
- plasma membrane Source: UniProtKB
Other locations
- cell surface Source: UniProtKB
- cilium Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 18 – 739 | ExtracellularSequence analysisAdd BLAST | 722 | |
Transmembranei | 740 – 760 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 761 – 804 | CytoplasmicSequence analysisAdd BLAST | 44 |
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | Sequence analysisAdd BLAST | 17 | |
ChainiPRO_0000028568 | 18 – 804 | Angiotensin-converting enzyme 2Add BLAST | 787 | |
ChainiPRO_0000292266 | 18 – 707 | Processed angiotensin-converting enzyme 2Add BLAST | 690 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 53 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 90 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 298 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 343 ↔ 360 | By similarity | ||
Glycosylationi | 431 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 529 ↔ 541 | By similarity | ||
Glycosylationi | 545 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 659 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 689 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 780 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 782 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q58DD0 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000034402, Expressed in diaphragm and 61 other tissues |
Interactioni
Subunit structurei
Homodimer.
Interacts with the catalytically active form of TMPRSS2 (By similarity).
Interacts with SLC6A19; this interaction is essential for expression and function of SLC6A19 in intestine (By similarity).
Interacts with ITGA5:ITGB1 (By similarity). Probably interacts (via endocytic sorting signal motif) with AP2M1; the interaction is inhibited by phosphorylation of Tyr-780 (By similarity).
Interacts (via PDZ-binding motif) with SLC9A3R1 (via PDZ domains); the interaction may enhance ACE2 membrane residence (By similarity).
By similarityBinary interactionsi
Q58DD0
With | #Exp. | IntAct |
---|---|---|
S [P0DTC2] from Severe acute respiratory syndrome coronavirus 2. | 3 | EBI-26998567,EBI-25474821 |
Protein-protein interaction databases
IntActi | Q58DD0, 3 interactors |
STRINGi | 9913.ENSBTAP00000045721 |
Chemistry databases
BindingDBi | Q58DD0 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 344 – 345 | Substrate bindingBy similarity | 2 | |
Regioni | 651 – 658 | Essential for cleavage by ADAM17By similarity | 8 | |
Regioni | 696 – 715 | Essential for cleavage by TMPRSS11D and TMPRSS2By similarityAdd BLAST | 20 | |
Regioni | 771 – 804 | DisorderedSequence analysisAdd BLAST | 34 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 777 – 785 | LIRBy similarity | 9 | |
Motifi | 780 – 784 | SH2-bindingBy similarity | 5 | |
Motifi | 780 – 783 | Endocytic sorting signalBy similarity | 4 | |
Motifi | 791 – 794 | PTBBy similarity | 4 | |
Motifi | 802 – 804 | PDZ-bindingBy similarity | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 775 – 804 | Polar residuesSequence analysisAdd BLAST | 30 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3690, Eukaryota |
HOGENOMi | CLU_014364_3_0_1 |
InParanoidi | Q58DD0 |
OrthoDBi | 422699at2759 |
TreeFami | TF312861 |
Family and domain databases
CDDi | cd06461, M2_ACE, 1 hit |
InterProi | View protein in InterPro IPR031588, Collectrin_dom IPR001548, Peptidase_M2 |
PANTHERi | PTHR10514, PTHR10514, 1 hit |
Pfami | View protein in Pfam PF16959, Collectrin, 1 hit PF01401, Peptidase_M2, 1 hit |
PRINTSi | PR00791, PEPDIPTASEA |
PROSITEi | View protein in PROSITE PS00142, ZINC_PROTEASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTGSFWLLLS LVAVTAAQST TEEQAKTFLE KFNHEAEDLS YQSSLASWNY
60 70 80 90 100
NTNITDENVQ KMNEARAKWS AFYEEQSRMA KTYSLEEIQN LTLKRQLKAL
110 120 130 140 150
QHSGTSALSA EKSKRLNTIL NKMSTIYSTG KVLDPNTQEC LALEPGLDDI
160 170 180 190 200
MENSRDYNRR LWAWEGWRAE VGKQLRPLYE EYVVLENEMA RANNYEDYGD
210 220 230 240 250
YWRGDYEVTG AGDYDYSRDQ LMKDVERTFA EIKPLYEQLH AYVRAKLMHT
260 270 280 290 300
YPSYISPTGC LPAHLLGDMW GRFWTNLYSL TVPFEHKPSI DVTEKMENQS
310 320 330 340 350
WDAERIFKEA EKFFVSISLP YMTQGFWDNS MLTEPGDGRK VVCHPTAWDL
360 370 380 390 400
GKGDFRIKMC TKVTMDDFLT AHHEMGHIQY DMAYAAQPYL LRNGANEGFH
410 420 430 440 450
EAVGEIMSLS AATPHYLKAL GLLAPDFHED NETEINFLLK QALTIVGTLP
460 470 480 490 500
FTYMLEKWRW MVFKGEIPKQ QWMEKWWEMK REIVGVVEPL PHDETYCDPA
510 520 530 540 550
CLFHVAEDYS FIRYYTRTIY QFQFHEALCK TAKHEGALFK CDISNSTEAG
560 570 580 590 600
QRLLQMLRLG KSEPWTLALE NIVGIKTMDV KPLLNYFEPL FTWLKEQNRN
610 620 630 640 650
SFVGWSTEWT PYSDQSIKVR ISLKSALGEN AYEWNDNEMY LFQSSVAYAM
660 670 680 690 700
RKYFSEARNE TVLFGEDNVW VSDKKPRISF KFFVTSPNNV SDIIPRTEVE
710 720 730 740 750
NAIRLSRDRI NDVFQLDDNS LEFLGIQPTL GPPYEPPVTI WLIIFGVVMG
760 770 780 790 800
VVVIGIVVLI FTGIRNRRKK NQASSEENPY GSVDLNKGEN NSGFQNIDDV
QTSL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BT021667 mRNA Translation: AAX46514.1 |
RefSeqi | XP_005228486.1, XM_005228429.3 |
Genome annotation databases
GeneIDi | 509235 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BT021667 mRNA Translation: AAX46514.1 |
RefSeqi | XP_005228486.1, XM_005228429.3 |
3D structure databases
SMRi | Q58DD0 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q58DD0, 3 interactors |
STRINGi | 9913.ENSBTAP00000045721 |
Chemistry databases
BindingDBi | Q58DD0 |
Protein family/group databases
MEROPSi | M02.006 |
Proteomic databases
PaxDbi | Q58DD0 |
Genome annotation databases
GeneIDi | 509235 |
Organism-specific databases
CTDi | 59272 |
Phylogenomic databases
eggNOGi | KOG3690, Eukaryota |
HOGENOMi | CLU_014364_3_0_1 |
InParanoidi | Q58DD0 |
OrthoDBi | 422699at2759 |
TreeFami | TF312861 |
Gene expression databases
Bgeei | ENSBTAG00000034402, Expressed in diaphragm and 61 other tissues |
Family and domain databases
CDDi | cd06461, M2_ACE, 1 hit |
InterProi | View protein in InterPro IPR031588, Collectrin_dom IPR001548, Peptidase_M2 |
PANTHERi | PTHR10514, PTHR10514, 1 hit |
Pfami | View protein in Pfam PF16959, Collectrin, 1 hit PF01401, Peptidase_M2, 1 hit |
PRINTSi | PR00791, PEPDIPTASEA |
PROSITEi | View protein in PROSITE PS00142, ZINC_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACE2_BOVIN | |
Accessioni | Q58DD0Primary (citable) accession number: Q58DD0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | April 26, 2005 | |
Last modified: | February 23, 2022 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families