UniProtKB - Q58839 (FEN_METJA)
Protein
Flap endonuclease 1
Gene
fen
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Functioni
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.
Cofactori
Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation
pH dependencei
Optimum pH is 6-7.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 27 | Magnesium 1UniRule annotation | 1 | |
Metal bindingi | 80 | Magnesium 1UniRule annotation | 1 | |
Metal bindingi | 152 | Magnesium 1Combined sources1 Publication | 1 | |
Metal bindingi | 154 | Magnesium 1Combined sources1 Publication | 1 | |
Metal bindingi | 173 | Magnesium 2UniRule annotation | 1 | |
Metal bindingi | 175 | Magnesium 2UniRule annotation | 1 | |
Metal bindingi | 224 | Magnesium 2Combined sources1 Publication | 1 |
GO - Molecular functioni
- 5'-3' exonuclease activity Source: UniProtKB-UniRule
- 5'-flap endonuclease activity Source: GO_Central
- DNA binding Source: UniProtKB-UniRule
- flap endonuclease activity Source: GO_Central
- magnesium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA repair Source: UniProtKB-UniRule
- DNA replication Source: UniProtKB-KW
- DNA replication, removal of RNA primer Source: UniProtKB-UniRule
Keywordsi
Molecular function | Endonuclease, Exonuclease, Hydrolase, Nuclease |
Biological process | DNA damage, DNA repair, DNA replication |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | MJAN243232:G1GKE-1566-MONOMER |
BRENDAi | 3.1.99.B1, 3260 |
Names & Taxonomyi
Protein namesi | Recommended name: Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)Short name: FEN-1UniRule annotation Alternative name(s): Flap structure-specific endonuclease 1UniRule annotation |
Gene namesi | Name:fenUniRule annotation Ordered Locus Names:MJ1444 |
Organismi | Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) |
Taxonomic identifieri | 243232 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanomada group › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154053 | 1 – 326 | Flap endonuclease 1Add BLAST | 326 |
Interactioni
Subunit structurei
Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.
Protein-protein interaction databases
STRINGi | 243232.MJ_1444 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q58839 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q58839 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 98 | N-domainAdd BLAST | 98 | |
Regioni | 116 – 245 | I-domainAdd BLAST | 130 | |
Regioni | 317 – 325 | Interaction with PCNAUniRule annotation | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | arCOG04050, Archaea |
HOGENOMi | CLU_032444_0_0_2 |
InParanoidi | Q58839 |
OMAi | CDYLDPI |
OrthoDBi | 41214at2157 |
PhylomeDBi | Q58839 |
Family and domain databases
HAMAPi | MF_00614, Fen, 1 hit |
InterProi | View protein in InterPro IPR036279, 5-3_exonuclease_C_sf IPR023426, Flap_endonuc IPR019973, Flap_structure-sp_endonuc_arc IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR006086, XPG-I_dom IPR006084, XPG/Rad2 IPR006085, XPG_DNA_repair_N |
PANTHERi | PTHR11081, PTHR11081, 1 hit |
Pfami | View protein in Pfam PF00867, XPG_I, 1 hit PF00752, XPG_N, 1 hit |
PRINTSi | PR00853, XPGRADSUPER |
SMARTi | View protein in SMART SM00279, HhH2, 1 hit SM00484, XPGI, 1 hit SM00485, XPGN, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR03674, fen_arch, 1 hit |
i Sequence
Sequence statusi: Complete.
Q58839-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGVQFGDFIP KNIISFEDLK GKKVAIDGMN ALYQFLTSIR LRDGSPLRNR
60 70 80 90 100
KGEITSAYNG VFYKTIHLLE NDITPIWVFD GEPPKLKEKT RKVRREMKEK
110 120 130 140 150
AELKMKEAIK KEDFEEAAKY AKRVSYLTPK MVENCKYLLS LMGIPYVEAP
160 170 180 190 200
SEGEAQASYM AKKGDVWAVV SQDYDALLYG APRVVRNLTT TKEMPELIEL
210 220 230 240 250
NEVLEDLRIS LDDLIDIAIF MGTDYNPGGV KGIGFKRAYE LVRSGVAKDV
260 270 280 290 300
LKKEVEYYDE IKRIFKEPKV TDNYSLSLKL PDKEGIIKFL VDENDFNYDR
310 320
VKKHVDKLYN LIANKTKQKT LDAWFK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L77117 Genomic DNA Translation: AAB99454.1 |
PIRi | C64480 |
RefSeqi | WP_010870964.1, NC_000909.1 |
Genome annotation databases
EnsemblBacteriai | AAB99454; AAB99454; MJ_1444 |
GeneIDi | 1452348 |
KEGGi | mja:MJ_1444 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L77117 Genomic DNA Translation: AAB99454.1 |
PIRi | C64480 |
RefSeqi | WP_010870964.1, NC_000909.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A76 | X-ray | 2.00 | A | 1-326 | [»] | |
1A77 | X-ray | 2.00 | A | 1-326 | [»] | |
SMRi | Q58839 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 243232.MJ_1444 |
Genome annotation databases
EnsemblBacteriai | AAB99454; AAB99454; MJ_1444 |
GeneIDi | 1452348 |
KEGGi | mja:MJ_1444 |
Phylogenomic databases
eggNOGi | arCOG04050, Archaea |
HOGENOMi | CLU_032444_0_0_2 |
InParanoidi | Q58839 |
OMAi | CDYLDPI |
OrthoDBi | 41214at2157 |
PhylomeDBi | Q58839 |
Enzyme and pathway databases
BioCyci | MJAN243232:G1GKE-1566-MONOMER |
BRENDAi | 3.1.99.B1, 3260 |
Miscellaneous databases
EvolutionaryTracei | Q58839 |
Family and domain databases
HAMAPi | MF_00614, Fen, 1 hit |
InterProi | View protein in InterPro IPR036279, 5-3_exonuclease_C_sf IPR023426, Flap_endonuc IPR019973, Flap_structure-sp_endonuc_arc IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR006086, XPG-I_dom IPR006084, XPG/Rad2 IPR006085, XPG_DNA_repair_N |
PANTHERi | PTHR11081, PTHR11081, 1 hit |
Pfami | View protein in Pfam PF00867, XPG_I, 1 hit PF00752, XPG_N, 1 hit |
PRINTSi | PR00853, XPGRADSUPER |
SMARTi | View protein in SMART SM00279, HhH2, 1 hit SM00484, XPGI, 1 hit SM00485, XPGN, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR03674, fen_arch, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FEN_METJA | |
Accessioni | Q58839Primary (citable) accession number: Q58839 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 5, 2001 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families