UniProtKB - Q58643 (SUCD_METJA)
Protein
Succinate--CoA ligase [ADP-forming] subunit alpha
Gene
sucD
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Functioni
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.UniRule annotation
Catalytic activityi
- EC:6.2.1.5UniRule annotation
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Succinate--CoA ligase [ADP-forming] subunit alpha (sucD), Succinate--CoA ligase [ADP-forming] subunit beta (sucC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 48 | Coenzyme AUniRule annotation | 1 | |
Binding sitei | 164 | Substrate; shared with subunit betaUniRule annotation | 1 | |
Active sitei | 251 | Tele-phosphohistidine intermediateUniRule annotation | 1 |
GO - Molecular functioni
- cofactor binding Source: InterPro
- nucleoside diphosphate kinase activity Source: GO_Central
- nucleotide binding Source: UniProtKB-KW
- succinate-CoA ligase (ADP-forming) activity Source: GO_Central
GO - Biological processi
- nucleoside triphosphate biosynthetic process Source: GO_Central
- succinate metabolic process Source: GO_Central
- succinyl-CoA metabolic process Source: GO_Central
- tricarboxylic acid cycle Source: GO_Central
Keywordsi
Molecular function | Ligase |
Biological process | Tricarboxylic acid cycle |
Ligand | Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER00999 |
Names & Taxonomyi
Protein namesi | Recommended name: Succinate--CoA ligase [ADP-forming] subunit alphaUniRule annotation (EC:6.2.1.5UniRule annotation)Alternative name(s): Succinyl-CoA synthetase subunit alphaUniRule annotation Short name: SCS-alphaUniRule annotation |
Gene namesi | Name:sucDUniRule annotation Ordered Locus Names:MJ1246 |
Organismi | Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) |
Taxonomic identifieri | 243232 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanomada group › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102810 | 1 – 294 | Succinate--CoA ligase [ADP-forming] subunit alphaAdd BLAST | 294 |
Interactioni
Subunit structurei
Heterotetramer of two alpha and two beta subunits.
UniRule annotationProtein-protein interaction databases
STRINGi | 243232.MJ_1246 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q58643 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q58643 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 22 – 25 | Coenzyme A bindingUniRule annotation | 4 | |
Regioni | 101 – 103 | Coenzyme A bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the succinate/malate CoA ligase alpha subunit family.UniRule annotation
Phylogenomic databases
eggNOGi | arCOG01339 Archaea COG0074 LUCA |
InParanoidi | Q58643 |
KOi | K01902 |
OMAi | IIFVPPA |
PhylomeDBi | Q58643 |
Family and domain databases
Gene3Di | 3.40.50.261, 1 hit |
HAMAPi | MF_01988 Succ_CoA_alpha, 1 hit |
InterProi | View protein in InterPro IPR017440 Cit_synth/succinyl-CoA_lig_AS IPR033847 Citrt_syn/SCS-alpha_CS IPR003781 CoA-bd IPR005810 CoA_lig_alpha IPR005811 CoA_ligase IPR036291 NAD(P)-bd_dom_sf IPR016102 Succinyl-CoA_synth-like |
Pfami | View protein in Pfam PF02629 CoA_binding, 1 hit PF00549 Ligase_CoA, 1 hit |
PIRSFi | PIRSF001553 SucCS_alpha, 1 hit |
SMARTi | View protein in SMART SM00881 CoA_binding, 1 hit |
SUPFAMi | SSF51735 SSF51735, 1 hit SSF52210 SSF52210, 1 hit |
TIGRFAMsi | TIGR01019 sucCoAalpha, 1 hit |
PROSITEi | View protein in PROSITE PS01216 SUCCINYL_COA_LIG_1, 1 hit PS00399 SUCCINYL_COA_LIG_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q58643-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLRDKMILL DENTKAIVQG ITGRQGSFHT KKMLECGTKI VGGVTPGKGG
60 70 80 90 100
QNVHGVPVFD TVKEAVKETD ANASVIFVPA PFAKDAVFEA IDAGIELIVV
110 120 130 140 150
ITEHIPVHDT MEFVNYAEDV GVKIIGPNTP GIASPKVGKL GIIPMEVLKE
160 170 180 190 200
GSVGMVSRSG TLTYEIAHQI KKAGFGVSTC VGIGGDPIVG LRYKEVLDLF
210 220 230 240 250
EKDDETEAIV MIGEIGGGAE EEAAKFIEKM KKPVIGYIAG QSAPEGKRMG
260 270 280 290
HAGAIVEKGK GTAESKMKAL EEAGAYVAKN ISDIPKLLAG ILGK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L77117 Genomic DNA Translation: AAB99250.1 |
PIRi | E64455 |
Genome annotation databases
EnsemblBacteriai | AAB99250; AAB99250; MJ_1246 |
KEGGi | mja:MJ_1246 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L77117 Genomic DNA Translation: AAB99250.1 |
PIRi | E64455 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YV1 | X-ray | 1.70 | A | 1-294 | [»] | |
SMRi | Q58643 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 243232.MJ_1246 |
Genome annotation databases
EnsemblBacteriai | AAB99250; AAB99250; MJ_1246 |
KEGGi | mja:MJ_1246 |
Phylogenomic databases
eggNOGi | arCOG01339 Archaea COG0074 LUCA |
InParanoidi | Q58643 |
KOi | K01902 |
OMAi | IIFVPPA |
PhylomeDBi | Q58643 |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER00999 |
Miscellaneous databases
EvolutionaryTracei | Q58643 |
Family and domain databases
Gene3Di | 3.40.50.261, 1 hit |
HAMAPi | MF_01988 Succ_CoA_alpha, 1 hit |
InterProi | View protein in InterPro IPR017440 Cit_synth/succinyl-CoA_lig_AS IPR033847 Citrt_syn/SCS-alpha_CS IPR003781 CoA-bd IPR005810 CoA_lig_alpha IPR005811 CoA_ligase IPR036291 NAD(P)-bd_dom_sf IPR016102 Succinyl-CoA_synth-like |
Pfami | View protein in Pfam PF02629 CoA_binding, 1 hit PF00549 Ligase_CoA, 1 hit |
PIRSFi | PIRSF001553 SucCS_alpha, 1 hit |
SMARTi | View protein in SMART SM00881 CoA_binding, 1 hit |
SUPFAMi | SSF51735 SSF51735, 1 hit SSF52210 SSF52210, 1 hit |
TIGRFAMsi | TIGR01019 sucCoAalpha, 1 hit |
PROSITEi | View protein in PROSITE PS01216 SUCCINYL_COA_LIG_1, 1 hit PS00399 SUCCINYL_COA_LIG_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SUCD_METJA | |
Accessioni | Q58643Primary (citable) accession number: Q58643 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 8, 2019 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Methanococcus jannaschii
Methanococcus jannaschii: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references