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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (apgM1), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (apgM2)
  4. Enolase (eno)
  5. Pyruvate kinase (MJ0108)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114NAD; via amide nitrogenBy similarity1
Active sitei144NucleophileBy similarity1
Binding sitei172NADBy similarity1
Binding sitei307NAD; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 13NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD, NADP

Enzyme and pathway databases

BioCyciMJAN243232:G1GKE-1242-MONOMER
BRENDAi1.2.1.59 3260
UniPathwayi
UPA00109;UER00184

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.59)
Short name:
GAPDH
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:gap
Ordered Locus Names:MJ1146
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001457231 – 343Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST343

Proteomic databases

PRIDEiQ58546

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi243232.MJ_1146

Structurei

Secondary structure

1343
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ58546
SMRiQ58546
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58546

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni143 – 145Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni198 – 199Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00493 Archaea
COG0057 LUCA
InParanoidiQ58546
KOiK00150
OMAiNAIVPNP
OrthoDBiPOG093Z05Z6
PhylomeDBiQ58546

Family and domain databases

HAMAPiMF_00559 G3P_dehdrog_arch, 1 hit
InterProiView protein in InterPro
IPR000846 DapB_N
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006436 Glyceraldehyde-3-P_DH_2_arc
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PTHR10836:SF76 PTHR10836:SF76, 1 hit
PfamiView protein in Pfam
PF01113 DapB_N, 1 hit
PF02800 Gp_dh_C, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01546 GAPDH-II_archae, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q58546-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPAKVLINGY GSIGKRVADA VSMQDDMEVI GVTKTKPDFE ARLAVEKGYK
60 70 80 90 100
LFVAIPDNER VKLFEDAGIP VEGTILDIIE DADIVVDGAP KKIGKQNLEN
110 120 130 140 150
IYKPHKVKAI LQGGEKAKDV EDNFNALWSY NRCYGKDYVR VVSCNTTGLC
160 170 180 190 200
RILYAINSIA DIKKARIVLV RRAADPNDDK TGPVNAITPN PVTVPSHHGP
210 220 230 240 250
DVVSVVPEFE GKILTSAVIV PTTLMHMHTL MVEVDGDVSR DDILEAIKKT
260 270 280 290 300
PRIITVRAED GFSSTAKIIE YGRDLGRLRY DINELVVWEE SINVLENEIF
310 320 330 340
LMQAVHQESI VIPENIDCIR AMLQMEEDNF KSIEKTNKAM GIQ
Length:343
Mass (Da):38,102
Last modified:November 1, 1996 - v1
Checksum:i81EB5810A9C838C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB99147.1
PIRiA64443
RefSeqiWP_010870657.1, NC_000909.1

Genome annotation databases

EnsemblBacteriaiAAB99147; AAB99147; MJ_1146
GeneIDi1452042
KEGGimja:MJ_1146

Similar proteinsi

Entry informationi

Entry nameiG3P_METJA
AccessioniPrimary (citable) accession number: Q58546
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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