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Protein

2-phospho-L-lactate guanylyltransferase

Gene

cofC

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanylyltransferase that catalyzes the activation of 2-phospho-L-lactate (LP) as (2S)-lactyl-2-diphospho-5'-guanosine (LPPG), via the condensation of LP with GTP. Is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor. Is able to utilize other purine nucleotides including dGTP and ITP as cosubstrates in place of GTP but with a lower activity. Does not display lactate kinase activity.1 Publication

Catalytic activityi

(2S)-2-phospholactate + GTP = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate.1 Publication

Kineticsi

  1. KM=36 µM for (2S)-2-phospholactate1 Publication
  2. KM=56 µM for GTP1 Publication
  1. Vmax=3 µmol/min/mg enzyme1 Publication

Temperature dependencei

Retains all activity when heated up to 90 degrees Celsius for 10 minutes and retained 38% activity after 10 minutes at 100 degrees Celsius.1 Publication

Pathwayi: coenzyme F420 biosynthesis

This protein is involved in the pathway coenzyme F420 biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway coenzyme F420 biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • phospholactate guanylyltransferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14615
BRENDAi2.7.7.68 3260
SABIO-RKiQ58297
UniPathwayi
UPA00071

Names & Taxonomyi

Protein namesi
Recommended name:
2-phospho-L-lactate guanylyltransferase (EC:2.7.7.68)
Short name:
LP guanylyltransferase
Gene namesi
Name:cofC
Ordered Locus Names:MJ0887
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomada groupMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001070911 – 2242-phospho-L-lactate guanylyltransferaseAdd BLAST224

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_0887

Structurei

3D structure databases

ProteinModelPortaliQ58297
SMRiQ58297
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CofC family.Curated

Phylogenomic databases

eggNOGiarCOG04472 Archaea
COG1920 LUCA
InParanoidiQ58297
KOiK14941
OMAiFRVDYHG
OrthoDBiPOG093Z0CT7
PhylomeDBiQ58297

Family and domain databases

Gene3Di3.90.550.10, 1 hit
HAMAPiMF_02114 CofC, 1 hit
InterProiView protein in InterPro
IPR002835 Coenzyme_F420_CofC
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR40392 PTHR40392, 1 hit
PfamiView protein in Pfam
PF01983 CofC, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR03552 F420_cofC, 1 hit

Sequencei

Sequence statusi: Complete.

Q58297-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNCGIKMKVI IPVSPINSLK TRLSEFLSGE ERKNLLLNML KDIIKALDGL
60 70 80 90 100
DIVIVSRDEE ILDFAKNELK AETIKEKYKG LNNAIKQAFE EIEDKEVIII
110 120 130 140 150
PADIPLIKKK HIEDILKLSK NYDLIIAPSR GGGTNLLYLK SKDLIEIKYE
160 170 180 190 200
GFSFLKHLEE AKKRNLRYYI YDSFLISVDI NTPEDLGEIF IHGNDTYTKN
210 220
YLKSLGIDVE PKHSSAGRFV VKRR
Length:224
Mass (Da):25,683
Last modified:November 1, 1996 - v1
Checksum:i0565EDD51F313278
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98891.1
PIRiG64410

Genome annotation databases

EnsemblBacteriaiAAB98891; AAB98891; MJ_0887
KEGGimja:MJ_0887

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98891.1
PIRiG64410

3D structure databases

ProteinModelPortaliQ58297
SMRiQ58297
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_0887

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98891; AAB98891; MJ_0887
KEGGimja:MJ_0887

Phylogenomic databases

eggNOGiarCOG04472 Archaea
COG1920 LUCA
InParanoidiQ58297
KOiK14941
OMAiFRVDYHG
OrthoDBiPOG093Z0CT7
PhylomeDBiQ58297

Enzyme and pathway databases

UniPathwayi
UPA00071

BioCyciMetaCyc:MONOMER-14615
BRENDAi2.7.7.68 3260
SABIO-RKiQ58297

Family and domain databases

Gene3Di3.90.550.10, 1 hit
HAMAPiMF_02114 CofC, 1 hit
InterProiView protein in InterPro
IPR002835 Coenzyme_F420_CofC
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR40392 PTHR40392, 1 hit
PfamiView protein in Pfam
PF01983 CofC, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR03552 F420_cofC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCOFC_METJA
AccessioniPrimary (citable) accession number: Q58297
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 10, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
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Main funding by: National Institutes of Health

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