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Protein

Endonuclease III

Gene

nth

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi184Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi190Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi193Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi201Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMJAN243232:G1GKE-659-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotation
Ordered Locus Names:MJ0613
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022431 – 344Endonuclease IIIAdd BLAST344

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_0613

Structurei

3D structure databases

ProteinModelPortaliQ58030
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini105 – 124HhHUniRule annotationAdd BLAST20
Domaini229 – 328GIY-YIGAdd BLAST100

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00459 Archaea
COG0177 LUCA
COG1833 LUCA
InParanoidiQ58030
KOiK10773
OMAiKLPKKYW
OrthoDBiPOG093Z0AWD
PhylomeDBiQ58030

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
cd10441 GIY-YIG_COG1833, 1 hit
Gene3Di1.10.1670.10, 1 hit
HAMAPiMF_00942 Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR002837 DUF123
IPR004036 Endonuclease-III-like_CS2
IPR000305 GIY-YIG_endonuc
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR005759 Nth
PfamiView protein in Pfam
PF01986 DUF123, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00465 GIYc, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
PROSITEiView protein in PROSITE
PS01155 ENDONUCLEASE_III_2, 1 hit
PS50164 GIY_YIG, 1 hit

Sequencei

Sequence statusi: Complete.

Q58030-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELIEILLKK LNKNAVVTEI AKDKDPFKVL ISTIISARTK DEVTEEVSKK
60 70 80 90 100
LFKEIKDVDD LLNIDEEKLA DLIYPAGFYK NKAKNLKKLA KILKENYNGK
110 120 130 140 150
VPDSLEELLK LPGVGRKTAN LVITLAFNKD GICVDTHVHR ICNRWEIVDT
160 170 180 190 200
ETPEETEFEL RKKLPKKYWK VINNLLVVFG REICSSKSKC DKCFKEIKEK
210 220 230 240 250
CPYYEKIKHF ENILKKFNFR KVSKNKIPNE KGTYILKIRL KEGKKIKFGK
260 270 280 290 300
TERFFKKGYY FYIGSAFGNS MNLKNRIERH LKDDKKMHWH IDYLLKYGKI
310 320 330 340
EEIYITNERV ECEVANEFIK KFDFVENFGC SDCKCKSHLF YLKP
Length:344
Mass (Da):40,568
Last modified:December 15, 1998 - v2
Checksum:iBC3BEFE1DE778B85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98606.1
RefSeqiWP_010870118.1, NC_000909.1

Genome annotation databases

EnsemblBacteriaiAAB98606; AAB98606; MJ_0613
GeneIDi1451479
KEGGimja:MJ_0613

Similar proteinsi

Entry informationi

Entry nameiEND3_METJA
AccessioniPrimary (citable) accession number: Q58030
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: May 23, 2018
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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