UniProtKB - Q57880 (TRM14_METJA)
Protein
tRNA (guanine(6)-N2)-methyltransferase
Gene
trm14
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Functioni
S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Cys).1 Publication
Catalytic activityi
- guanosine6 in tRNA + S-adenosyl-L-methionine = H+ + N2-methylguanosine6 in tRNA + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.2561 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 261 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 306 | S-adenosyl-L-methionineBy similarity | 1 |
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
- tRNA (guanine) methyltransferase activity Source: UniProtKB
GO - Biological processi
- tRNA methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | MJAN243232:G1GKE-468-MONOMER |
BRENDAi | 2.1.1.256, 3260 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanine(6)-N2)-methyltransferaseCurated (EC:2.1.1.2561 Publication)Alternative name(s): tRNA m2G6-methyltransferaseCurated |
Gene namesi | Name:trm141 Publication Ordered Locus Names:MJ0438 |
Organismi | Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) |
Taxonomic identifieri | 243232 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanomada group › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000140480 | 1 – 381 | tRNA (guanine(6)-N2)-methyltransferaseAdd BLAST | 381 |
Proteomic databases
PRIDEi | Q57880 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 43 – 157 | THUMPPROSITE-ProRule annotationAdd BLAST | 115 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 173 – 177 | S-adenosyl-L-methionine bindingBy similarity | 5 | |
Regioni | 204 – 206 | S-adenosyl-L-methionine bindingBy similarity | 3 | |
Regioni | 289 – 290 | S-adenosyl-L-methionine bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the methyltransferase superfamily.Curated
Phylogenomic databases
eggNOGi | arCOG00048, Archaea |
HOGENOMi | CLU_032119_0_0_2 |
InParanoidi | Q57880 |
OMAi | GFMYKAN |
OrthoDBi | 47556at2157 |
PhylomeDBi | Q57880 |
Family and domain databases
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR000241, RNA_methylase_dom IPR029063, SAM-dependent_MTases IPR004114, THUMP_dom |
Pfami | View protein in Pfam PF02926, THUMP, 1 hit PF01170, UPF0020, 1 hit |
SMARTi | View protein in SMART SM00981, THUMP, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit PS51165, THUMP, 1 hit PS01261, UPF0020, 1 hit |
i Sequence
Sequence statusi: Complete.
Q57880-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDYYVTLSPG LEKISKNEIE SFGGKIKEIR ENKGRIFFSG DLKLIPKINY
60 70 80 90 100
LSRTIERMNI LLHREEIPNI ALDDIYKRVY NIDWTEWIKE NQSFAIRPLR
110 120 130 140 150
AGEHNFTSID IGRVAGEAVI KSYQRDKNIR LKVNLDEPDV IVRVEVIFDE
160 170 180 190 200
LIVGIDTTGD IALDKRGYRV FNHPAHLNAT IASSLVYLSD WKDDEMLLDP
210 220 230 240 250
MCGSGTIPIE GALMKRNIPP GKFRENKYGF KFIDIFGYEL LDKIKKEIVE
260 270 280 290 300
NKNIYKIIGL DKNQKYLDGA KDNAKNAEVL DTIEFICGDA TKLHEKFNES
310 320 330 340 350
DVIIANPPYG IRIGSKRSVK KLYDEFLSSA KEIMHGSSRL IVITAEDKMF
360 370 380
KDAIAKNNFE VKEEFNVMFG GLMTRVFYLT L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L77117 Genomic DNA Translation: AAB98426.1 |
PIRi | F64354 |
RefSeqi | WP_010869937.1, NC_000909.1 |
Genome annotation databases
EnsemblBacteriai | AAB98426; AAB98426; MJ_0438 |
GeneIDi | 1451298 |
KEGGi | mja:MJ_0438 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L77117 Genomic DNA Translation: AAB98426.1 |
PIRi | F64354 |
RefSeqi | WP_010869937.1, NC_000909.1 |
3D structure databases
SMRi | Q57880 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 243232.MJ_0438 |
Proteomic databases
PRIDEi | Q57880 |
Genome annotation databases
EnsemblBacteriai | AAB98426; AAB98426; MJ_0438 |
GeneIDi | 1451298 |
KEGGi | mja:MJ_0438 |
Phylogenomic databases
eggNOGi | arCOG00048, Archaea |
HOGENOMi | CLU_032119_0_0_2 |
InParanoidi | Q57880 |
OMAi | GFMYKAN |
OrthoDBi | 47556at2157 |
PhylomeDBi | Q57880 |
Enzyme and pathway databases
BioCyci | MJAN243232:G1GKE-468-MONOMER |
BRENDAi | 2.1.1.256, 3260 |
Family and domain databases
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR000241, RNA_methylase_dom IPR029063, SAM-dependent_MTases IPR004114, THUMP_dom |
Pfami | View protein in Pfam PF02926, THUMP, 1 hit PF01170, UPF0020, 1 hit |
SMARTi | View protein in SMART SM00981, THUMP, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit PS51165, THUMP, 1 hit PS01261, UPF0020, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRM14_METJA | |
Accessioni | Q57880Primary (citable) accession number: Q57880 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families