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Entry version 147 (29 Sep 2021)
Sequence version 1 (01 Nov 1997)
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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=15 µM for tRNA(Tyr) (at 45 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei32Tyrosine1 Publication1
Binding sitei36Tyrosine1 Publication1
Binding sitei173Tyrosine1 Publication1
Binding sitei207ATPSequence analysis1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MJAN243232:G1GKE-416-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
6.1.1.1, 3260

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
Q57834

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Tyrosine--tRNA ligase (EC:6.1.1.1)
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name:
TyrRS
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:tyrS
Ordered Locus Names:MJ0389
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri243232 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaMethanomada groupMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000805 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi32Y → Q: Confers specificity for the non-natural amino acid O-methyl-tyrosine; when associated with T-107; A-158 and P-162. 1
Mutagenesisi107E → T: Confers specificity for the non-natural amino acid O-methyl-tyrosine; when associated with Q-32; A-158 and P-162. 1
Mutagenesisi158D → A: Confers specificity for the non-natural amino acid O-methyl-tyrosine; when associated with Q-32; T-107 and P-162. 1
Mutagenesisi162L → P: Confers specificity for the non-natural amino acid O-methyl-tyrosine; when associated with Q-32; T-107 and A-158. 1
Mutagenesisi286D → A: Decreases the rate of aminoacylation more than 10-fold, without effect on tyrosyl adenylate synthesis. 2 Publications1
Mutagenesisi286D → R: Decreases the rate of aminoacylation with wild-type tRNA and increases aminoacylation with amber suppressor tRNA 8-fold. Decreases affinity for wild-type tRNA and increases affinity for amber suppressor tRNA. 2 Publications1
Mutagenesisi288K → A: Decreases the rate of aminoacylation more than 200-fold, without effect on tyrosyl adenylate synthesis. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000556691 – 306Tyrosine--tRNA ligaseAdd BLAST306

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei143Interaction with t-RNA1

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
243232.MJ_0389

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1306
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q57834

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q57834

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni151 – 158Tyrosine8
Regioni228 – 231Interaction with t-RNA4
Regioni283 – 288Interaction with t-RNA6

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi37 – 45'HIGH' region9
Motifi204 – 208'KMSKS' region5

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
arCOG01886, Archaea

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_035267_0_1_2

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q57834

Identification of Orthologs from Complete Genome Data

More...
OMAi
YIGFEIS

Database of Orthologous Groups

More...
OrthoDBi
59062at2157

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q57834

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00805, TyrRS_core, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.620, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_02008, Tyr_tRNA_synth_type3, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR001412, aa-tRNA-synth_I_CS
IPR002305, aa-tRNA-synth_Ic
IPR014729, Rossmann-like_a/b/a_fold
IPR002307, Tyr-tRNA-ligase
IPR023684, Tyr-tRNA-ligase_3
IPR023617, Tyr-tRNA-ligase_arc/euk-type

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00579, tRNA-synt_1b, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF006588, TyrRS_arch_euk, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01040, TRNASYNTHTYR

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00234, tyrS, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00178, AA_TRNA_LIGASE_I, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q57834-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDEFEMIKRN TSEIISEEEL REVLKKDEKS AYIGFEPSGK IHLGHYLQIK
60 70 80 90 100
KMIDLQNAGF DIIILLADLH AYLNQKGELD EIRKIGDYNK KVFEAMGLKA
110 120 130 140 150
KYVYGSEFQL DKDYTLNVYR LALKTTLKRA RRSMELIARE DENPKVAEVI
160 170 180 190 200
YPIMQVNDIH YLGVDVAVGG MEQRKIHMLA RELLPKKVVC IHNPVLTGLD
210 220 230 240 250
GEGKMSSSKG NFIAVDDSPE EIRAKIKKAY CPAGVVEGNP IMEIAKYFLE
260 270 280 290 300
YPLTIKRPEK FGGDLTVNSY EELESLFKNK ELHPMDLKNA VAEELIKILE

PIRKRL
Length:306
Mass (Da):35,049
Last modified:November 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1887760ABAF2DD5E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98375.1

Protein sequence database of the Protein Information Resource

More...
PIRi
E64348

NCBI Reference Sequences

More...
RefSeqi
WP_010869888.1, NC_000909.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAB98375; AAB98375; MJ_0389

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1451246

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mja:MJ_0389

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98375.1
PIRiE64348
RefSeqiWP_010869888.1, NC_000909.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J1UX-ray1.95A1-306[»]
1U7DX-ray2.65A/B1-306[»]
1U7XX-ray3.00A/B1-306[»]
1ZH0X-ray1.90A1-306[»]
1ZH6X-ray2.50A1-306[»]
2AG6X-ray1.90A1-306[»]
2HGZX-ray2.50A2-306[»]
2PXHX-ray1.97A1-306[»]
2ZP1X-ray1.70A1-306[»]
3D6UX-ray2.20A1-306[»]
3D6VX-ray2.20A1-306[»]
3N2YX-ray2.49A/B1-306[»]
3QE4X-ray2.30A/B1-306[»]
4HJRX-ray2.50A/B1-306[»]
4HJXX-ray2.91A/B1-306[»]
4HK4X-ray2.30A1-306[»]
4HPWX-ray2.00A1-306[»]
4ND6X-ray2.00A1-306[»]
4ND7X-ray2.00A1-306[»]
4NDAX-ray1.70A1-306[»]
4NX2X-ray2.00A1-306[»]
4PBRX-ray1.90A1-306[»]
4PBSX-ray2.01A1-306[»]
4PBTX-ray1.90A1-306[»]
5L7PX-ray1.90A/B1-306[»]
5N5UX-ray1.60A1-306[»]
5N5VX-ray2.30A/B/C/D/E/F/G/H1-306[»]
5NSFX-ray2.43A/B/C/D1-306[»]
5U36X-ray3.03A/B1-306[»]
6WRKX-ray1.95A1-306[»]
6WRNX-ray1.60A1-306[»]
6WRQX-ray1.85A1-306[»]
6WRTX-ray1.55A1-306[»]
7C5CX-ray1.72A1-306[»]
7CKGX-ray2.05A/B1-306[»]
7CKHX-ray1.79A/B1-306[»]
SMRiQ57834
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_0389

Genome annotation databases

EnsemblBacteriaiAAB98375; AAB98375; MJ_0389
GeneIDi1451246
KEGGimja:MJ_0389

Phylogenomic databases

eggNOGiarCOG01886, Archaea
HOGENOMiCLU_035267_0_1_2
InParanoidiQ57834
OMAiYIGFEIS
OrthoDBi59062at2157
PhylomeDBiQ57834

Enzyme and pathway databases

BioCyciMJAN243232:G1GKE-416-MONOMER
BRENDAi6.1.1.1, 3260
SABIO-RKiQ57834

Miscellaneous databases

EvolutionaryTraceiQ57834

Family and domain databases

CDDicd00805, TyrRS_core, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_02008, Tyr_tRNA_synth_type3, 1 hit
InterProiView protein in InterPro
IPR001412, aa-tRNA-synth_I_CS
IPR002305, aa-tRNA-synth_Ic
IPR014729, Rossmann-like_a/b/a_fold
IPR002307, Tyr-tRNA-ligase
IPR023684, Tyr-tRNA-ligase_3
IPR023617, Tyr-tRNA-ligase_arc/euk-type
PfamiView protein in Pfam
PF00579, tRNA-synt_1b, 1 hit
PIRSFiPIRSF006588, TyrRS_arch_euk, 1 hit
PRINTSiPR01040, TRNASYNTHTYR
TIGRFAMsiTIGR00234, tyrS, 1 hit
PROSITEiView protein in PROSITE
PS00178, AA_TRNA_LIGASE_I, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYY_METJA
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q57834
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 29, 2021
This is version 147 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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