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UniProtKB - Q57599 (RNH2_METJA)
Protein
Ribonuclease HII
Gene
rnhB
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Functioni
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
Catalytic activityi
- Endonucleolytic cleavage to 5'-phosphomonoester. EC:3.1.26.4
Cofactori
Mn2+By similarity, Mg2+By similarityNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 7 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 8 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 112 | Divalent metal cationBy similarity | 1 |
GO - Molecular functioni
- manganese ion binding Source: UniProtKB-UniRule
- RNA binding Source: InterPro
- RNA-DNA hybrid ribonuclease activity Source: GO_Central
GO - Biological processi
- DNA replication, removal of RNA primer Source: GO_Central
- mismatch repair Source: GO_Central
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Ligand | Magnesium, Manganese, Metal-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rnhB Ordered Locus Names:MJ0135 |
Organismi | Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) |
Taxonomic identifieri | 243232 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanomada group › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
- ribonuclease H2 complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 7 | D → N: Reduces activity 800-fold. 1 Publication | 1 | |
Mutagenesisi | 8 | E → Q: Reduces activity 700-fold. 1 Publication | 1 | |
Mutagenesisi | 112 | D → N: Reduces activity 600-fold. 1 Publication | 1 | |
Mutagenesisi | 149 | D → N: Reduces activity 800-fold. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000111663 | 1 – 230 | Ribonuclease HIIAdd BLAST | 230 |
Proteomic databases
PRIDEi | Q57599 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q57599 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q57599 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 224 | RNase H type-2PROSITE-ProRule annotationAdd BLAST | 224 |
Sequence similaritiesi
Belongs to the RNase HII family.Curated
Phylogenomic databases
eggNOGi | arCOG04121, Archaea |
HOGENOMi | CLU_036532_0_4_2 |
InParanoidi | Q57599 |
OMAi | REECRFF |
OrthoDBi | 105421at2157 |
PhylomeDBi | Q57599 |
Family and domain databases
Gene3Di | 1.10.10.460, 1 hit 3.30.420.10, 1 hit |
HAMAPi | MF_00052_A, RNase_HII_A, 1 hit |
InterProi | View protein in InterPro IPR004649, RNase_H2_suA IPR001352, RNase_HII/HIII IPR024567, RNase_HII/HIII_dom IPR020787, RNase_HII_arc IPR023160, RNase_HII_hlx-loop-hlx_cap_dom IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10954, PTHR10954, 1 hit |
Pfami | View protein in Pfam PF01351, RNase_HII, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit |
TIGRFAMsi | TIGR00729, TIGR00729, 1 hit |
PROSITEi | View protein in PROSITE PS51975, RNASE_H_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q57599-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIIIGIDEAG RGPVLGPMVV CAFAIEKERE EELKKLGVKD SKELTKNKRA
60 70 80 90 100
YLKKLLENLG YVEKRILEAE EINQLMNSIN LNDIEINAFS KVAKNLIEKL
110 120 130 140 150
NIRDDEIEIY IDACSTNTKK FEDSFKDKIE DIIKERNLNI KIIAEHKADA
160 170 180 190 200
KYPVVSAASI IAKAERDEII DYYKKIYGDI GSGYPSDPKT IKFLEDYFKK
210 220 230
HKKLPDIART HWKTCKRILD KSKQTKLIIE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L77117 Genomic DNA Translation: AAB98116.1 |
PIRi | G64316 |
RefSeqi | WP_010869628.1, NC_000909.1 |
Genome annotation databases
EnsemblBacteriai | AAB98116; AAB98116; MJ_0135 |
GeneIDi | 1450976 |
KEGGi | mja:MJ_0135 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L77117 Genomic DNA Translation: AAB98116.1 |
PIRi | G64316 |
RefSeqi | WP_010869628.1, NC_000909.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EKE | X-ray | 2.00 | A/B | 1-230 | [»] | |
SMRi | Q57599 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 243232.MJ_0135 |
Proteomic databases
PRIDEi | Q57599 |
Genome annotation databases
EnsemblBacteriai | AAB98116; AAB98116; MJ_0135 |
GeneIDi | 1450976 |
KEGGi | mja:MJ_0135 |
Phylogenomic databases
eggNOGi | arCOG04121, Archaea |
HOGENOMi | CLU_036532_0_4_2 |
InParanoidi | Q57599 |
OMAi | REECRFF |
OrthoDBi | 105421at2157 |
PhylomeDBi | Q57599 |
Miscellaneous databases
EvolutionaryTracei | Q57599 |
Family and domain databases
Gene3Di | 1.10.10.460, 1 hit 3.30.420.10, 1 hit |
HAMAPi | MF_00052_A, RNase_HII_A, 1 hit |
InterProi | View protein in InterPro IPR004649, RNase_H2_suA IPR001352, RNase_HII/HIII IPR024567, RNase_HII/HIII_dom IPR020787, RNase_HII_arc IPR023160, RNase_HII_hlx-loop-hlx_cap_dom IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10954, PTHR10954, 1 hit |
Pfami | View protein in Pfam PF01351, RNase_HII, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit |
TIGRFAMsi | TIGR00729, TIGR00729, 1 hit |
PROSITEi | View protein in PROSITE PS51975, RNASE_H_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RNH2_METJA | |
Accessioni | Q57599Primary (citable) accession number: Q57599 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families