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UniProtKB - Q57598 (TRMI_METJA)
Protein
tRNA (adenine(57)-N(1)/adenine(58)-N(1))-methyltransferase TrmI
Gene
trmI
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Functioni
Catalyzes the S-adenosyl-L-methionine-dependent formation of N1-methyladenine at position 58 (m1A58) in tRNA.
By similarityCatalytic activityi
- adenosine57/adenosine58 in tRNA + 2 S-adenosyl-L-methionine = 2 H+ + N1-methyladenosine57/N1-methyladenosine58 in tRNA + 2 S-adenosyl-L-homocysteinePROSITE-ProRule annotationEC:2.1.1.219PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 119 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 172 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 188 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- tRNA (adenine-N1-)-methyltransferase activity Source: InterPro
GO - Biological processi
- tRNA methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (adenine(57)-N(1)/adenine(58)-N(1))-methyltransferase TrmI (EC:2.1.1.219)Alternative name(s): tRNA(m1A57/58)-methyltransferase |
Gene namesi | Name:trmI Ordered Locus Names:MJ0134 |
Organismi | Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) |
Taxonomic identifieri | 243232 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanomada group › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106712 | 1 – 282 | tRNA (adenine(57)-N(1)/adenine(58)-N(1))-methyltransferase TrmIAdd BLAST | 282 |
Interactioni
Subunit structurei
Homotetramer composed of a dimer of dimers.
By similarityProtein-protein interaction databases
STRINGi | 243232.MJ_0134 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 98 – 101 | S-adenosyl-L-methionine bindingBy similarity | 4 |
Domaini
Contains a large catalytic C-terminal domain that binds S-adenosyl-L-methionine and a smaller N-terminal domain that may play a role in tRNA recognition. Domains are connected by a linker region.By similarity
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | arCOG00978, Archaea |
HOGENOMi | CLU_025402_0_1_2 |
InParanoidi | Q57598 |
OMAi | VVYPKDA |
OrthoDBi | 64860at2157 |
PhylomeDBi | Q57598 |
Family and domain databases
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR014816, tRNA_MeTrfase_Gcd14 |
PANTHERi | PTHR12133, PTHR12133, 1 hit |
Pfami | View protein in Pfam PF08704, GCD14, 1 hit |
PIRSFi | PIRSF017269, GCD14, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51620, SAM_TRM61, 1 hit |
i Sequence
Sequence statusi: Complete.
Q57598-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFAYKLLVDE RGKRYLLKKN VEKFGTDLGI VDMKDIEEGV ELKSHKGHTF
60 70 80 90 100
YLVEPTMFDI LKRMKRTVTT LLPKDIGFII ARAGIREGET VVEAGTGSGA
110 120 130 140 150
LTMYLSNAVG KTGKVITYDI RPEFAKVARK NLLRVGAIKK GQKIIGLDEE
160 170 180 190 200
FDDEDEIEIE DGLFNVIQKI GDVREKIDEK DVDVIVLDLP DPWNVVENAK
210 220 230 240 250
KALNKKRGRI VTYLPYIEQV KKTVEKLKEE GFWDIHTYEI IEREIEISEK
260 270 280
GVRPSTRMIG HTGYITVARV PPEPLDREEE KE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L77117 Genomic DNA Translation: AAB98115.1 |
PIRi | F64316 |
RefSeqi | WP_010869627.1, NC_000909.1 |
Genome annotation databases
EnsemblBacteriai | AAB98115; AAB98115; MJ_0134 |
GeneIDi | 1450975 |
KEGGi | mja:MJ_0134 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L77117 Genomic DNA Translation: AAB98115.1 |
PIRi | F64316 |
RefSeqi | WP_010869627.1, NC_000909.1 |
3D structure databases
SMRi | Q57598 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 243232.MJ_0134 |
Genome annotation databases
EnsemblBacteriai | AAB98115; AAB98115; MJ_0134 |
GeneIDi | 1450975 |
KEGGi | mja:MJ_0134 |
Phylogenomic databases
eggNOGi | arCOG00978, Archaea |
HOGENOMi | CLU_025402_0_1_2 |
InParanoidi | Q57598 |
OMAi | VVYPKDA |
OrthoDBi | 64860at2157 |
PhylomeDBi | Q57598 |
Family and domain databases
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR014816, tRNA_MeTrfase_Gcd14 |
PANTHERi | PTHR12133, PTHR12133, 1 hit |
Pfami | View protein in Pfam PF08704, GCD14, 1 hit |
PIRSFi | PIRSF017269, GCD14, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51620, SAM_TRM61, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRMI_METJA | |
Accessioni | Q57598Primary (citable) accession number: Q57598 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families