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Protein

Glutaminase liver isoform, mitochondrial

Gene

Gls2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production (By similarity).By similarity

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei219SubstrateBy similarity1
Binding sitei268SubstrateBy similarity1
Binding sitei314SubstrateBy similarity1
Binding sitei321SubstrateBy similarity1
Binding sitei347SubstrateBy similarity1
Binding sitei399SubstrateBy similarity1
Binding sitei417Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

  • glutaminase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-MMU-210500 Glutamate Neurotransmitter Release Cycle
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-70614 Amino acid synthesis and interconversion (transamination)

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase liver isoform, mitochondrial (EC:3.5.1.2)
Short name:
GLS
Alternative name(s):
L-glutaminase
L-glutamine amidohydrolase
Gene namesi
Name:Gls2
Synonyms:Kiaa4146
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2143539 Gls2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176860

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 14MitochondrionSequence analysisAdd BLAST14
ChainiPRO_000001162615 – 602Glutaminase liver isoform, mitochondrialAdd BLAST588

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253N6-succinyllysineCombined sources1
Modified residuei279N6-acetyllysineCombined sources1
Modified residuei284N6-acetyllysineCombined sources1
Modified residuei329N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ571F8
PaxDbiQ571F8
PeptideAtlasiQ571F8
PRIDEiQ571F8

PTM databases

iPTMnetiQ571F8
PhosphoSitePlusiQ571F8
SwissPalmiQ571F8

Expressioni

Gene expression databases

BgeeiENSMUSG00000044005 Expressed in 129 organ(s), highest expression level in liver
CleanExiMM_GLS2
ExpressionAtlasiQ571F8 baseline and differential
GenevisibleiQ571F8 MM

Interactioni

Subunit structurei

Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with the PDZ domain of TAX1BP3 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ571F8, 1 interactor
STRINGi10090.ENSMUSP00000047376

Chemistry databases

BindingDBiQ571F8

Structurei

3D structure databases

ProteinModelPortaliQ571F8
SMRiQ571F8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati518 – 551ANK 1Add BLAST34
Repeati552 – 585ANK 2Add BLAST34

Sequence similaritiesi

Belongs to the glutaminase family.Curated

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0506 Eukaryota
COG2066 LUCA
GeneTreeiENSGT00390000010463
HOGENOMiHOG000216891
HOVERGENiHBG005856
InParanoidiQ571F8
KOiK01425
OMAiGNSHRGI
OrthoDBiEOG091G03YU
PhylomeDBiQ571F8
TreeFamiTF313359

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
HAMAPiMF_00313 Glutaminase, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR012338 Beta-lactam/transpept-like
IPR015868 Glutaminase
PANTHERiPTHR12544 PTHR12544, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF04960 Glutaminase, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 2 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56601 SSF56601, 1 hit
TIGRFAMsiTIGR03814 Gln_ase, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q571F8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRSMRALQNA LSRAGSHGRR GGWGHPSRGP LLGRGVRYYL GEAAAQGRGT
60 70 80 90 100
PHSHQPQHSD HDASHSGMLP RLGDLLFYTI AEGQERIPIH KFTTALKATG
110 120 130 140 150
LQTSDPRLQD CMSKMQRMVQ ESSSGGLLDR ELFQKCVSSN IVLLTQAFRK
160 170 180 190 200
KFVIPDFEEF TGHVDRIFED AKEPTGGKVA AYIPHLAKSN PDLWGVSLCT
210 220 230 240 250
VDGQRHSVGH TKIPFCLQSC VKPLTYAISV STLGTDYVHK FVGKEPSGLR
260 270 280 290 300
YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF VLQYLNKMAG
310 320 330 340 350
NEFMGFSNAT FQSEKETGDR NYAIGYYLKE KKCFPKGVDM MAALDLYFQL
360 370 380 390 400
CSVEVTCESG SVMAATLANG GICPITGESV LSAEAVRNTL SLMHSCGMYD
410 420 430 440 450
FSGQFAFHVG LPAKSAVSGA ILLVVPNVMG MMCLSPPLDK LGNSQRGINF
460 470 480 490 500
CQKLVSLFNF HNYDNLRHCA RKLDPRREGG EVRNKTVVNL LFAAYSGDVS
510 520 530 540 550
ALRRFALSAM DMEQKDYDSR TALHVAAAEG HIEVVKFLIE ACKVNPFVKD
560 570 580 590 600
RWGNIPLDDA VQFNHLEVVK LLQDYHDSYL LSETQAEAAA ETLSKENLES

MV
Length:602
Mass (Da):66,366
Last modified:August 30, 2005 - v2
Checksum:iBA07D22ADB082F76
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6SJZ7F6SJZ7_MOUSE
Glutaminase liver isoform, mitochon...
Gls2
176Annotation score:
D6RI88D6RI88_MOUSE
Glutaminase liver isoform, mitochon...
Gls2
186Annotation score:
E0CYF7E0CYF7_MOUSE
Glutaminase liver isoform, mitochon...
Gls2
136Annotation score:
F6WPK7F6WPK7_MOUSE
Glutaminase liver isoform, mitochon...
Gls2
201Annotation score:
E0CXA4E0CXA4_MOUSE
Glutaminase liver isoform, mitochon...
Gls2
135Annotation score:

Sequence cautioni

The sequence BAD90156 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312Q → K in BAE38445 (PubMed:16141072).Curated1
Sequence conflicti584T → S in AAI41116 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220231 mRNA Translation: BAD90156.1 Different initiation.
AK165896 mRNA Translation: BAE38445.1
BC141115 mRNA Translation: AAI41116.1
CCDSiCCDS24263.1
RefSeqiNP_001028436.2, NM_001033264.3
UniGeneiMm.30102
Mm.447148

Genome annotation databases

EnsembliENSMUST00000044776; ENSMUSP00000047376; ENSMUSG00000044005
GeneIDi216456
KEGGimmu:216456
UCSCiuc007hlo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220231 mRNA Translation: BAD90156.1 Different initiation.
AK165896 mRNA Translation: BAE38445.1
BC141115 mRNA Translation: AAI41116.1
CCDSiCCDS24263.1
RefSeqiNP_001028436.2, NM_001033264.3
UniGeneiMm.30102
Mm.447148

3D structure databases

ProteinModelPortaliQ571F8
SMRiQ571F8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ571F8, 1 interactor
STRINGi10090.ENSMUSP00000047376

Chemistry databases

BindingDBiQ571F8
ChEMBLiCHEMBL2176860

PTM databases

iPTMnetiQ571F8
PhosphoSitePlusiQ571F8
SwissPalmiQ571F8

Proteomic databases

MaxQBiQ571F8
PaxDbiQ571F8
PeptideAtlasiQ571F8
PRIDEiQ571F8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044776; ENSMUSP00000047376; ENSMUSG00000044005
GeneIDi216456
KEGGimmu:216456
UCSCiuc007hlo.2 mouse

Organism-specific databases

CTDi27165
MGIiMGI:2143539 Gls2
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0506 Eukaryota
COG2066 LUCA
GeneTreeiENSGT00390000010463
HOGENOMiHOG000216891
HOVERGENiHBG005856
InParanoidiQ571F8
KOiK01425
OMAiGNSHRGI
OrthoDBiEOG091G03YU
PhylomeDBiQ571F8
TreeFamiTF313359

Enzyme and pathway databases

ReactomeiR-MMU-210500 Glutamate Neurotransmitter Release Cycle
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-70614 Amino acid synthesis and interconversion (transamination)

Miscellaneous databases

PROiPR:Q571F8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000044005 Expressed in 129 organ(s), highest expression level in liver
CleanExiMM_GLS2
ExpressionAtlasiQ571F8 baseline and differential
GenevisibleiQ571F8 MM

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
HAMAPiMF_00313 Glutaminase, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR012338 Beta-lactam/transpept-like
IPR015868 Glutaminase
PANTHERiPTHR12544 PTHR12544, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF04960 Glutaminase, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 2 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56601 SSF56601, 1 hit
TIGRFAMsiTIGR03814 Gln_ase, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGLSL_MOUSE
AccessioniPrimary (citable) accession number: Q571F8
Secondary accession number(s): B2RUF2, Q3TMJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: November 7, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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