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UniProtKB - Q57071 (PTG3C_STACT)
Protein
PTS system glucose-specific EIICBA component
Gene
ptsG
Organism
Staphylococcus carnosus (strain TM300)
Status
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport. Cannot transport galactose, fructose, mannose, cellobiose, sucrose, maltose, lactose, melibiose and trehalose, as well as N-acetylglucosamine.
1 PublicationCatalytic activityi
- D-glucose(out) + Nπ-phospho-L-histidyl-[protein] = D-glucose 6-phosphate(in) + L-histidyl-[protein]1 PublicationEC:2.7.1.1991 Publication
Activity regulationi
Inhibited by 2-deoxyglucose and methyl beta-D-glucoside, but not by methyl alpha-D-glucoside, p-nitrophenyl alpha-D-glucoside, o-nitrophenyl beta-D-glucoside and salicin.1 Publication
Kineticsi
- KM=12 µM for glucose1 Publication
- KM=30.5 µM for glucose (in the presence of Triton X-100)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 447 | Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation | 1 | |
Active sitei | 599 | Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- glucose transmembrane transporter activity Source: InterPro
- kinase activity Source: UniProtKB-KW
- protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: InterPro
GO - Biological processi
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Protein family/group databases
TCDBi | 4.A.1.1.13, the pts glucose-glucoside (glc) family |
Names & Taxonomyi
Protein namesi | Recommended name: PTS system glucose-specific EIICBA component1 Publication (EC:2.7.1.1991 Publication)Alternative name(s): EIICBA-Glc1 Publication Short name: EII-Glc1 Publication EIICBA-Glc 1Curated Including the following 3 domains: Glucose permease IIC component1 Publication Alternative name(s): PTS system glucose-specific EIIC component1 Publication Glucose-specific phosphotransferase enzyme IIB component1 Publication Alternative name(s): PTS system glucose-specific EIIB component1 Publication Glucose-specific phosphotransferase enzyme IIA component1 Publication Alternative name(s): PTS system glucose-specific EIIA component1 Publication |
Gene namesi | Name:ptsG Synonyms:glcA1 Publication Ordered Locus Names:Sca_0999 |
Organismi | Staphylococcus carnosus (strain TM300) |
Taxonomic identifieri | 396513 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane PROSITE-ProRule annotation1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 16 – 36 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 63 – 83 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 89 – 109 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 126 – 146 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 170 – 190 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 199 – 219 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 273 – 293 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 303 – 323 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 329 – 349 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 355 – 375 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 383 – 403 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000351401 | 1 – 675 | PTS system glucose-specific EIICBA componentAdd BLAST | 675 |
Expressioni
Inductioni
Up-regulated by the antiterminator protein GlcT.1 Publication
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 414 | PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST | 412 | |
Domaini | 425 – 506 | PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 547 – 651 | PTS EIIA type-1PROSITE-ProRule annotationAdd BLAST | 105 |
Domaini
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.PROSITE-ProRule annotation
The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1263, Bacteria COG1264, Bacteria COG2190, Bacteria |
HOGENOMi | CLU_012312_1_1_9 |
OMAi | AWAFNRF |
OrthoDBi | 2035550at2 |
Family and domain databases
CDDi | cd00212, PTS_IIB_glc, 1 hit |
Gene3Di | 2.70.70.10, 1 hit 3.30.1360.60, 1 hit |
InterProi | View protein in InterPro IPR011055, Dup_hybrid_motif IPR036878, Glu_permease_IIB IPR018113, PTrfase_EIIB_Cys IPR001127, PTS_EIIA_1_perm IPR003352, PTS_EIIC IPR013013, PTS_EIIC_1 IPR001996, PTS_IIB_1 IPR011299, PTS_IIBC_glc |
Pfami | View protein in Pfam PF00358, PTS_EIIA_1, 1 hit PF00367, PTS_EIIB, 1 hit PF02378, PTS_EIIC, 1 hit |
SUPFAMi | SSF51261, SSF51261, 1 hit SSF55604, SSF55604, 1 hit |
TIGRFAMsi | TIGR00826, EIIB_glc, 1 hit TIGR00830, PTBA, 1 hit TIGR02002, PTS-II-BC-glcB, 1 hit |
PROSITEi | View protein in PROSITE PS51093, PTS_EIIA_TYPE_1, 1 hit PS00371, PTS_EIIA_TYPE_1_HIS, 1 hit PS51098, PTS_EIIB_TYPE_1, 1 hit PS01035, PTS_EIIB_TYPE_1_CYS, 1 hit PS51103, PTS_EIIC_TYPE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q57071-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWKKFFGQLQ RIGKALMLPV AILPAAGLLL ALGNAFQGDA LQSLMPFIKA
60 70 80 90 100
EGFQNVAKMM EGAGGIIFDN LAIIFALGVA IGLASGDGVA AIAAFVGFIV
110 120 130 140 150
LNKTMGMFLG VTPEKAADAA TGFANVLGIP TLQTGVFGGI IIGALAAWCY
160 170 180 190 200
NKFYNISLPS YLGFFAGKRF VPIMMATCSF ILAFPMAIIW PSIQGGLNAF
210 220 230 240 250
SEGLLASNTG LAVFLFGFIK RLLIPFGLHH IFHAPFWFEF GSYKNAAGQI
260 270 280 290 300
IHGDQRIFIE QIRDNVPLTA GKFMQGEFPV MMFGLPAAAL AIYQTAKKEN
310 320 330 340 350
KKVVAGLMLS GALTSFLTGI TEPLEFSFLF VAPLLFFIHA VLDGLSFLIL
360 370 380 390 400
YLLDLHLGYT FSGGFIDFFL LGILPNKTQW WLVIPVGLVY AAIYYIIFRF
410 420 430 440 450
LIVKFNFKTP GREDKEVKSS NVAASELPFK VLDAMGGKAN IKHLDACITR
460 470 480 490 500
LRVEVNDKAK VDVQELKDLG ASGVLEVGNN MQAIFGPKSD QIKHDMQQIM
510 520 530 540 550
DGKITSPEET TVTEEGDKET AEIAAAGGGV VYAPIKGEVV DISEVPDKVF
560 570 580 590 600
SEKMMGDGIA IKPETGEVVA PFDGVVKMVF PTKHAIGLES KDGIELLIHF
610 620 630 640 650
GLETVKLEGK GFDILVKEND NIVLGQPLMK VDLDYIKEHA DSTITPIVVT
660 670
NLNGRTMEVL QHGEVKQGDK VILVK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X93360 Genomic DNA Translation: CAA63742.1 AM295250 Genomic DNA Translation: CAL27907.1 |
PIRi | S46952 |
RefSeqi | WP_015900248.1, NC_012121.1 |
Genome annotation databases
GeneIDi | 60545310 |
KEGGi | sca:SCA_0999 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X93360 Genomic DNA Translation: CAA63742.1 AM295250 Genomic DNA Translation: CAL27907.1 |
PIRi | S46952 |
RefSeqi | WP_015900248.1, NC_012121.1 |
3D structure databases
SMRi | Q57071 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 396513.SCA_0999 |
Protein family/group databases
TCDBi | 4.A.1.1.13, the pts glucose-glucoside (glc) family |
Genome annotation databases
GeneIDi | 60545310 |
KEGGi | sca:SCA_0999 |
Phylogenomic databases
eggNOGi | COG1263, Bacteria COG1264, Bacteria COG2190, Bacteria |
HOGENOMi | CLU_012312_1_1_9 |
OMAi | AWAFNRF |
OrthoDBi | 2035550at2 |
Family and domain databases
CDDi | cd00212, PTS_IIB_glc, 1 hit |
Gene3Di | 2.70.70.10, 1 hit 3.30.1360.60, 1 hit |
InterProi | View protein in InterPro IPR011055, Dup_hybrid_motif IPR036878, Glu_permease_IIB IPR018113, PTrfase_EIIB_Cys IPR001127, PTS_EIIA_1_perm IPR003352, PTS_EIIC IPR013013, PTS_EIIC_1 IPR001996, PTS_IIB_1 IPR011299, PTS_IIBC_glc |
Pfami | View protein in Pfam PF00358, PTS_EIIA_1, 1 hit PF00367, PTS_EIIB, 1 hit PF02378, PTS_EIIC, 1 hit |
SUPFAMi | SSF51261, SSF51261, 1 hit SSF55604, SSF55604, 1 hit |
TIGRFAMsi | TIGR00826, EIIB_glc, 1 hit TIGR00830, PTBA, 1 hit TIGR02002, PTS-II-BC-glcB, 1 hit |
PROSITEi | View protein in PROSITE PS51093, PTS_EIIA_TYPE_1, 1 hit PS00371, PTS_EIIA_TYPE_1_HIS, 1 hit PS51098, PTS_EIIB_TYPE_1, 1 hit PS01035, PTS_EIIB_TYPE_1_CYS, 1 hit PS51103, PTS_EIIC_TYPE_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PTG3C_STACT | |
Accessioni | Q57071Primary (citable) accession number: Q57071 Secondary accession number(s): B9DP62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 14, 2008 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |