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Protein

Serine--glyoxylate aminotransferase

Gene

AGT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Photorespiratory enzyme that catalyzes transamination reactions with multiple substrates, including asparagine. Function exclusively as a catabolic enzyme in Asn metabolism.2 Publications

Miscellaneous

Preferentially acts as a serine--glyoxylate aminotransferase in vitro.

Catalytic activityi

L-serine + glyoxylate = 3-hydroxypyruvate + glycine.2 Publications
L-alanine + glyoxylate = pyruvate + glycine.2 Publications
L-serine + pyruvate = 3-hydroxypyruvate + L-alanine.1 Publication

Cofactori

Activity regulationi

Inhibited by aminooxyacetate and beta-chloro-L-alanine, but not by p-hydroxymercuribenzoate.1 Publication

Kineticsi

kcat is 6.4 sec(-1) for L-serine. kcat is 2.86 sec(-1) for L-alanine.
  1. KM=0.87 mM for alanine (with recombinant enzyme, in the presence of 20 mM glyoxylate)2 Publications
  2. KM=1.08 mM for alanine (with recombinant enzyme, in the presence of 20 mM hydroxypyruvate)2 Publications
  3. KM=2.47 mM for serine (with recombinant enzyme, in the presence of 20 mM glyoxylate)2 Publications
  4. KM=0.99 mM for serine (with recombinant enzyme, in the presence of 20 mM pyruvate)2 Publications
  5. KM=0.59 mM for glycine (with recombinant enzyme, in the presence of 20 mM pyruvate)2 Publications
  6. KM=1.58 mM for glycine (with recombinant enzyme, in the presence of 1 mM hydroxypyruvate)2 Publications
  7. KM=2.82 mM for asparagine (with recombinant enzyme, in the presence of 20 mM glyoxylate)2 Publications
  8. KM=3.79 mM for asparagine (with recombinant enzyme, in the presence of 20 mM hydroxypyruvate)2 Publications
  9. KM=5.0 mM for L-serine (with native enzyme, in the presence of 10 mM glyoxylate)2 Publications
  10. KM=1.53 mM for L-serine (with native enzyme, in the presence of 0.5 mM glyoxylate)2 Publications
  11. KM=0.91 mM for glyoxylate (with native enzyme, in the presence of 30 mM L-serine)2 Publications
  12. KM=2.83 mM for glycine (with native enzyme, in the presence of 0.5 mM hydroxypyruvate)2 Publications
  13. KM=0.57 mM for hydroxypyruvate (with native enzyme, in the presence of 15.4 mM glycine)2 Publications
  14. KM=1.25 mM for L-alanine (with native enzyme, in the presence of 10 mM glyoxylate)2 Publications
  15. KM=0.58 mM for L-alanine (with native enzyme, in the presence of 0.5 mM glyoxylate)2 Publications
  16. KM=0.50 mM for glyoxylate (with native enzyme, in the presence of 30 mM L-alanine)2 Publications
  17. KM=1.25 mM for glycine (with native enzyme, in the presence of 0.5 mM pyruvate)2 Publications
  18. KM=0.22 mM for pyruvate (with native enzyme, in the presence of 15.4 mM glycine)2 Publications
  1. Vmax=50.2 nmol/sec/mg enzyme with alanine as substrate (with recombinant enzyme, in the presence of 20 mM glyoxylate)2 Publications
  2. Vmax=73.8 nmol/sec/mg enzyme with alanine as substrate (with recombinant enzyme, in the presence of 20 mM hydroxypyruvate)2 Publications
  3. Vmax=33.2 nmol/sec/mg enzyme with serine as substrate (with recombinant enzyme, in the presence of 20 mM glyoxylate)2 Publications
  4. Vmax=37.0 nmol/sec/mg enzyme with serine as substrate (with recombinant enzyme, in the presence of 20 mM pyruvate)2 Publications
  5. Vmax=8.4 nmol/sec/mg enzyme with glycine as substrate (with recombinant enzyme, in the presence of 20 mM pyruvate)2 Publications
  6. Vmax=3.8 nmol/sec/mg enzyme with glycine as substrate (with recombinant enzyme, in the presence of 1 mM hydroxypyruvate)2 Publications
  7. Vmax=292 nmol/sec/mg enzyme with asparagine as substrate (with recombinant enzyme, in the presence of 20 mM glyoxylate)2 Publications
  8. Vmax=704 nmol/sec/mg enzyme with asparagine as substrate (with recombinant enzyme, in the presence of 20 mM pyruvate)2 Publications
  9. Vmax=358 nmol/sec/mg enzyme with asparagine as substrate (with recombinant enzyme, in the presence of 20 mM hydroxypyruvate)2 Publications
  10. Vmax=7.64 µmol/min/mg enzyme with L-serine as substrate (with native enzyme, in the presence of 10 mM glyoxylate)2 Publications
  11. Vmax=3.42 µmol/min/mg enzyme with L-alanine as substrate (with native enzyme, in the presence of 10 mM glyoxylate)2 Publications

pH dependencei

Optimum pH is 9.2.2 Publications

GO - Molecular functioni

GO - Biological processi

  • glycine biosynthetic process, by transamination of glyoxylate Source: GO_Central
  • photorespiration Source: TAIR

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processPhotorespiration
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:AT2G13360-MONOMER
BRENDAi2.6.1.44 399
2.6.1.45 399
ReactomeiR-ATH-389661 Glyoxylate metabolism and glycine degradation
SABIO-RKiQ56YA5

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--glyoxylate aminotransferase (EC:2.6.1.452 Publications)
Alternative name(s):
Alanine--glyoxylate aminotransferase (EC:2.6.1.442 Publications)
Short name:
AGT
Asparagine aminotransferase (EC:2.6.1.-)
Serine--pyruvate aminotransferase (EC:2.6.1.511 Publication)
Gene namesi
Name:AGT1
Ordered Locus Names:At2g13360
ORF Names:F14O4.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G13360
TAIRilocus:2041649 AT2G13360

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi251P → L: Abolishes aminotransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001502341 – 401Serine--glyoxylate aminotransferaseAdd BLAST401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei201N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei204PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ56YA5
PRIDEiQ56YA5

PTM databases

iPTMnetiQ56YA5

Expressioni

Tissue specificityi

Ubiquitous. Preferentially expressed in green, leafy tissues, root cortex and epidermis, developing siliques and dry seeds.2 Publications

Inductioni

Up-regulated in roots after treatment with asparagine.1 Publication

Gene expression databases

ExpressionAtlasiQ56YA5 baseline and differential
GenevisibleiQ56YA5 AT

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi1183, 4 interactors
IntActiQ56YA5, 4 interactors
MINTiQ56YA5
STRINGi3702.AT2G13360.1

Structurei

3D structure databases

ProteinModelPortaliQ56YA5
SMRiQ56YA5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi399 – 401Microbody targeting signalSequence analysis3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2862 Eukaryota
COG0075 LUCA
HOGENOMiHOG000171814
InParanoidiQ56YA5
KOiK00830
OMAiWGCDDKP
OrthoDBiEOG09360C7Z
PhylomeDBiQ56YA5

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR000192 Aminotrans_V_dom
IPR020578 Aminotrans_V_PyrdxlP_BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
IPR024169 SP_NH2Trfase/AEP_transaminase
PfamiView protein in Pfam
PF00266 Aminotran_5, 1 hit
PIRSFiPIRSF000524 SPT, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00595 AA_TRANSFER_CLASS_5, 1 hit

Sequencei

Sequence statusi: Complete.

Q56YA5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDYMYGPGRH HLFVPGPVNI PEPVIRAMNR NNEDYRSPAI PALTKTLLED
60 70 80 90 100
VKKIFKTTSG TPFLFPTTGT GAWESALTNT LSPGDRIVSF LIGQFSLLWI
110 120 130 140 150
DQQKRLNFNV DVVESDWGQG ANLQVLASKL SQDENHTIKA ICIVHNETAT
160 170 180 190 200
GVTNDISAVR TLLDHYKHPA LLLVDGVSSI CALDFRMDEW GVDVALTGSQ
210 220 230 240 250
KALSLPTGLG IVCASPKALE ATKTSKSLKV FFDWNDYLKF YKLGTYWPYT
260 270 280 290 300
PSIQLLYGLR AALDLIFEEG LENIIARHAR LGKATRLAVE AWGLKNCTQK
310 320 330 340 350
EEWISNTVTA VMVPPHIDGS EIVRRAWQRY NLSLGLGLNK VAGKVFRIGH
360 370 380 390 400
LGNVNELQLL GCLAGVEMIL KDVGYPVVMG SGVAAASTYL QHHIPLIPSR

I
Length:401
Mass (Da):44,208
Last modified:September 13, 2005 - v2
Checksum:i608E7EFD840C84D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063901 mRNA Translation: AAC26854.1
AB048945 mRNA Translation: BAB20811.1
AC007209 Genomic DNA Translation: AAD28669.1
CP002685 Genomic DNA Translation: AEC06227.1
CP002685 Genomic DNA Translation: AEC06228.1
CP002685 Genomic DNA Translation: ANM62628.1
AY096498 mRNA Translation: AAM20136.1
AY114010 mRNA Translation: AAM45058.1
AK221418 mRNA Translation: BAD94403.1
PIRiT52250
RefSeqiNP_001318216.1, NM_001335388.1
NP_178969.1, NM_126925.5
NP_849951.1, NM_179620.2
UniGeneiAt.24853

Genome annotation databases

EnsemblPlantsiAT2G13360.1; AT2G13360.1; AT2G13360
AT2G13360.2; AT2G13360.2; AT2G13360
AT2G13360.3; AT2G13360.3; AT2G13360
GeneIDi815822
GrameneiAT2G13360.1; AT2G13360.1; AT2G13360
AT2G13360.2; AT2G13360.2; AT2G13360
AT2G13360.3; AT2G13360.3; AT2G13360
KEGGiath:AT2G13360

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063901 mRNA Translation: AAC26854.1
AB048945 mRNA Translation: BAB20811.1
AC007209 Genomic DNA Translation: AAD28669.1
CP002685 Genomic DNA Translation: AEC06227.1
CP002685 Genomic DNA Translation: AEC06228.1
CP002685 Genomic DNA Translation: ANM62628.1
AY096498 mRNA Translation: AAM20136.1
AY114010 mRNA Translation: AAM45058.1
AK221418 mRNA Translation: BAD94403.1
PIRiT52250
RefSeqiNP_001318216.1, NM_001335388.1
NP_178969.1, NM_126925.5
NP_849951.1, NM_179620.2
UniGeneiAt.24853

3D structure databases

ProteinModelPortaliQ56YA5
SMRiQ56YA5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1183, 4 interactors
IntActiQ56YA5, 4 interactors
MINTiQ56YA5
STRINGi3702.AT2G13360.1

PTM databases

iPTMnetiQ56YA5

Proteomic databases

PaxDbiQ56YA5
PRIDEiQ56YA5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G13360.1; AT2G13360.1; AT2G13360
AT2G13360.2; AT2G13360.2; AT2G13360
AT2G13360.3; AT2G13360.3; AT2G13360
GeneIDi815822
GrameneiAT2G13360.1; AT2G13360.1; AT2G13360
AT2G13360.2; AT2G13360.2; AT2G13360
AT2G13360.3; AT2G13360.3; AT2G13360
KEGGiath:AT2G13360

Organism-specific databases

AraportiAT2G13360
TAIRilocus:2041649 AT2G13360

Phylogenomic databases

eggNOGiKOG2862 Eukaryota
COG0075 LUCA
HOGENOMiHOG000171814
InParanoidiQ56YA5
KOiK00830
OMAiWGCDDKP
OrthoDBiEOG09360C7Z
PhylomeDBiQ56YA5

Enzyme and pathway databases

BioCyciMetaCyc:AT2G13360-MONOMER
BRENDAi2.6.1.44 399
2.6.1.45 399
ReactomeiR-ATH-389661 Glyoxylate metabolism and glycine degradation
SABIO-RKiQ56YA5

Miscellaneous databases

PROiPR:Q56YA5

Gene expression databases

ExpressionAtlasiQ56YA5 baseline and differential
GenevisibleiQ56YA5 AT

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR000192 Aminotrans_V_dom
IPR020578 Aminotrans_V_PyrdxlP_BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
IPR024169 SP_NH2Trfase/AEP_transaminase
PfamiView protein in Pfam
PF00266 Aminotran_5, 1 hit
PIRSFiPIRSF000524 SPT, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00595 AA_TRANSFER_CLASS_5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSGAT_ARATH
AccessioniPrimary (citable) accession number: Q56YA5
Secondary accession number(s): O81248
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: September 12, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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Main funding by: National Institutes of Health

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