UniProtKB - Q56Y52 (POT1A_ARATH)
Protection of telomeres protein 1a
POT1A
Functioni
Caution
GO - Molecular functioni
- G-rich strand telomeric DNA binding Source: GO_Central
- RNA binding Source: TAIR
- single-stranded telomeric DNA binding Source: UniProtKB
- telomerase inhibitor activity Source: GO_Central
- telomeric DNA binding Source: TAIR
GO - Biological processi
- negative regulation of telomerase activity Source: GO_Central
- positive regulation of telomere maintenance Source: UniProtKB
- positive regulation of telomere maintenance via telomerase Source: UniProtKB
- regulation of telomere maintenance via telomerase Source: GO_Central
- telomere capping Source: GO_Central
- telomere maintenance Source: TAIR
Keywordsi
Molecular function | DNA-binding, RNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Protection of telomeres protein 1a1 PublicationShort name: AtPOT1a1 Publication Short name: AtPot11 Publication Alternative name(s): Protection of telomeres protein 11 Publication |
Gene namesi | Name:POT1A1 Publication Synonyms:POT11 Publication Ordered Locus Names:At2g05210Imported ORF Names:F5G3 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G05210 |
TAIRi | locus:2051214, AT2G05210 |
Subcellular locationi
Nucleus
- nuclear chromosome, telomeric region Source: InterPro
- nuclear telomere cap complex Source: GO_Central
- nucleolus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chromosome, Cytoplasm, Nucleus, TelomerePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 35 | E → F: Reduces POT1A function in positive regulation of telomere length; reduces binding affinity for CTC1; has no effect on binding affinity for STN1. 1 Publication | 1 | |
Mutagenesisi | 65 | F → A: Almost abolishes POT1A function in positive regulation of telomere length; abolishes telomeric DNA binding. 2 Publications | 1 | |
Mutagenesisi | 132 | L → D: Has no effect on POT1A function in positive regulation of telomere length; has no effect on binding affinity for CTC1. 1 Publication | 1 | |
Mutagenesisi | 221 | S → A: Reduces POT1A function in positive regulation of telomere length; reduces binding affinity for CTC1; has no effect on binding affinity for STN1. 1 Publication | 1 | |
Mutagenesisi | 297 | E → F: Has minor effect on POT1A function in positive regulation of telomere length, reduces binding affinity for CTC1; has no effect on binding affinity for STN1. 1 Publication | 1 | |
Mutagenesisi | 458 – 467 | Missing : Abolishes POT1A function in positive regulation of telomere length. 1 Publication | 10 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000416957 | 1 – 467 | Protection of telomeres protein 1aAdd BLAST | 467 |
Proteomic databases
PaxDbi | Q56Y52 |
PRIDEi | Q56Y52 |
ProMEXi | Q56Y52 |
ProteomicsDBi | 250482 [Q56Y52-1] |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | Q56Y52, baseline and differential |
Genevisiblei | Q56Y52, AT |
Interactioni
Subunit structurei
Component of the telomerase holoenzyme complex at least composed of TERT, CBF5 and POT1a (PubMed:18212040) (Probable). The RNA molecule associated to the telomerase complex, and providing a template for telomeric DNA synthesis, is most likely TR and not TER1 as described previously (PubMed:31754033, PubMed:31392988, PubMed:31754031).
Interacts with the N-terminal part of TERT (PubMed:17911168).
Interacts with CBF5 (PubMed:18212040, PubMed:30834599).
Interacts with CTC1 and STN1 (PubMed:25329641, PubMed:25697340). Does not interact with TEN1 (PubMed:25329641).
1 Publication8 PublicationsBinary interactionsi
Q56Y52
With | #Exp. | IntAct |
---|---|---|
TERT [Q9SPU7] | 4 | EBI-1606062,EBI-1606133 |
Protein-protein interaction databases
BioGRIDi | 469, 6 interactors |
IntActi | Q56Y52, 4 interactors |
STRINGi | 3702.AT2G05210.2 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4757, Eukaryota |
HOGENOMi | CLU_020958_0_1_1 |
InParanoidi | Q56Y52 |
OMAi | FRCEERY |
OrthoDBi | 940962at2759 |
PhylomeDBi | Q56Y52 |
Family and domain databases
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR028389, POT1 IPR011564, Telomer_end-bd_POT1/Cdc13 |
PANTHERi | PTHR14513, PTHR14513, 1 hit |
Pfami | View protein in Pfam PF02765, POT1, 1 hit |
SMARTi | View protein in SMART SM00976, Telo_bind, 1 hit |
SUPFAMi | SSF50249, SSF50249, 2 hits |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKKRESPKL IKIKDAIKLI NQQVSLIGIV LEQREPKQCR NNDWICTLRI
60 70 80 90 100
IDDTYPSPGL TVNVFSKTLE QLPQIKNHDD MILFTRIKMQ TFDSGERVNA
110 120 130 140 150
ACSRWVSSFA LFEGVDFVCY QCSTNFHEEE ALYKSAMDDL RKVFAGCSQV
160 170 180 190 200
IKAMQSISYR TKPCSEVFSF LREIKIGKRF DLVCRILHAD EDTSAVFVWD
210 220 230 240 250
GTDAPPASIL AKRSEEDKAF SSLSVHTLLS RDVLLSFPTV GTILRVHLSS
260 270 280 290 300
HLFYRVKPGD WVKLYHLLCE VDRGSWVIKV TSSTKVHHLA QDDRLVEKIM
310 320 330 340 350
RIYDKRLSSK LGHISFWCFP SPPGLTETDD NCAPFVTLMD IITFPKVTCK
360 370 380 390 400
YRCIVRVVAA YPWQVEDFCS DENRRHHQVL LTLEDSTATL EAFLCNKDAE
410 420 430 440 450
YFWGLGFQDT ETLRKKRNWL LGIRESSNFV APRNPPWIEC CILSYYTNKA
460
DPWNTRLYRI FGTRLLH
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 292 | D → G in AAS59561 (PubMed:15589838).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_043057 | 186 – 199 | ILHAD…AVFVW → VICSILSINYSPCR in isoform 2. CuratedAdd BLAST | 14 | |
Alternative sequenceiVSP_043058 | 186 – 199 | ILHAD…AVFVW → VICSILSINCEKAF in isoform 3. CuratedAdd BLAST | 14 | |
Alternative sequenceiVSP_043059 | 200 – 467 | Missing in isoform 2 and isoform 3. CuratedAdd BLAST | 268 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY553323 mRNA Translation: AAS59561.1 AB195819 Genomic DNA Translation: BAD99146.1 AB195819 Genomic DNA Translation: BAD99147.1 AB195819 Genomic DNA Translation: BAD99148.1 AY884593 mRNA Translation: AAX78213.2 AC007018 Genomic DNA Translation: AAD29059.1 Sequence problems. CP002685 Genomic DNA Translation: AEC05904.1 CP002685 Genomic DNA Translation: AEC05905.1 CP002685 Genomic DNA Translation: AEC05906.1 AK221471 mRNA Translation: BAD94597.1 |
PIRi | A84466 |
RefSeqi | NP_001118270.1, NM_001124798.2 [Q56Y52-1] NP_001118271.1, NM_001124799.2 [Q56Y52-1] NP_178592.3, NM_126547.5 [Q56Y52-1] |
Genome annotation databases
EnsemblPlantsi | AT2G05210.1; AT2G05210.1; AT2G05210 [Q56Y52-1] AT2G05210.2; AT2G05210.2; AT2G05210 [Q56Y52-1] AT2G05210.3; AT2G05210.3; AT2G05210 [Q56Y52-1] |
GeneIDi | 815069 |
Gramenei | AT2G05210.1; AT2G05210.1; AT2G05210 [Q56Y52-1] AT2G05210.2; AT2G05210.2; AT2G05210 [Q56Y52-1] AT2G05210.3; AT2G05210.3; AT2G05210 [Q56Y52-1] |
KEGGi | ath:AT2G05210 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY553323 mRNA Translation: AAS59561.1 AB195819 Genomic DNA Translation: BAD99146.1 AB195819 Genomic DNA Translation: BAD99147.1 AB195819 Genomic DNA Translation: BAD99148.1 AY884593 mRNA Translation: AAX78213.2 AC007018 Genomic DNA Translation: AAD29059.1 Sequence problems. CP002685 Genomic DNA Translation: AEC05904.1 CP002685 Genomic DNA Translation: AEC05905.1 CP002685 Genomic DNA Translation: AEC05906.1 AK221471 mRNA Translation: BAD94597.1 |
PIRi | A84466 |
RefSeqi | NP_001118270.1, NM_001124798.2 [Q56Y52-1] NP_001118271.1, NM_001124799.2 [Q56Y52-1] NP_178592.3, NM_126547.5 [Q56Y52-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 469, 6 interactors |
IntActi | Q56Y52, 4 interactors |
STRINGi | 3702.AT2G05210.2 |
Proteomic databases
PaxDbi | Q56Y52 |
PRIDEi | Q56Y52 |
ProMEXi | Q56Y52 |
ProteomicsDBi | 250482 [Q56Y52-1] |
Genome annotation databases
EnsemblPlantsi | AT2G05210.1; AT2G05210.1; AT2G05210 [Q56Y52-1] AT2G05210.2; AT2G05210.2; AT2G05210 [Q56Y52-1] AT2G05210.3; AT2G05210.3; AT2G05210 [Q56Y52-1] |
GeneIDi | 815069 |
Gramenei | AT2G05210.1; AT2G05210.1; AT2G05210 [Q56Y52-1] AT2G05210.2; AT2G05210.2; AT2G05210 [Q56Y52-1] AT2G05210.3; AT2G05210.3; AT2G05210 [Q56Y52-1] |
KEGGi | ath:AT2G05210 |
Organism-specific databases
Araporti | AT2G05210 |
TAIRi | locus:2051214, AT2G05210 |
Phylogenomic databases
eggNOGi | KOG4757, Eukaryota |
HOGENOMi | CLU_020958_0_1_1 |
InParanoidi | Q56Y52 |
OMAi | FRCEERY |
OrthoDBi | 940962at2759 |
PhylomeDBi | Q56Y52 |
Miscellaneous databases
PROi | PR:Q56Y52 |
Gene expression databases
ExpressionAtlasi | Q56Y52, baseline and differential |
Genevisiblei | Q56Y52, AT |
Family and domain databases
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR028389, POT1 IPR011564, Telomer_end-bd_POT1/Cdc13 |
PANTHERi | PTHR14513, PTHR14513, 1 hit |
Pfami | View protein in Pfam PF02765, POT1, 1 hit |
SMARTi | View protein in SMART SM00976, Telo_bind, 1 hit |
SUPFAMi | SSF50249, SSF50249, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | POT1A_ARATH | |
Accessioni | Q56Y52Primary (citable) accession number: Q56Y52 Secondary accession number(s): Q4W6W9 Q9SJ37 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 18, 2012 |
Last sequence update: | May 10, 2005 | |
Last modified: | August 12, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families