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Protein

Ubiquinol oxidase 4, chloroplastic/chromoplastic

Gene

AOX4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts early in chloroplast biogenesis as a component of a redox chain responsible for phytoene desaturation. Prevents the generation of toxic oxygen radicals and photooxidation of the nascent photosynthetic apparatus. Involved in the differentiation of multiple plastid types, including chloroplasts, amyloplasts, and etioplasts. Might participate in the chloroplast respiratory chain.10 Publications

Catalytic activityi

2 ubiquinol + O2 = 2 ubiquinone + 2 H2O.2 Publications

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi136Iron 1By similarity1
Metal bindingi175Iron 1By similarity1
Metal bindingi175Iron 2By similarity1
Metal bindingi178Iron 1; via pros nitrogenBy similarity1
Metal bindingi227Iron 2By similarity1
Metal bindingi296Iron 1By similarity1
Metal bindingi296Iron 2By similarity1
Metal bindingi299Iron 2By similarity1

GO - Molecular functioni

  • alternative oxidase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • ubiquinol:oxygen oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  • carotenoid biosynthetic process Source: TAIR
  • plastid organization Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processCarotenoid biosynthesis, Electron transport, Respiratory chain, Transport
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquinol oxidase 4, chloroplastic/chromoplastic (EC:1.10.3.11)
Alternative name(s):
Alternative oxidase 4
Plastid terminal oxidase
Protein IMMUTANS
Gene namesi
Name:AOX4
Synonyms:IM, PTOX
Ordered Locus Names:At4g22260
ORF Names:T10I14_90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G22260
TAIRilocus:2005514 AT4G22260

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Transmembranei195 – 215HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Chloroplast, Chromoplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Variegated cotyledons and leaves. The amount of white tissue increases with light intensity.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi136E → A, D or H: Abolishes cyanide-resistant oxygen consumption. 2 Publications1
Mutagenesisi175E → A, D or H: Abolishes cyanide-resistant oxygen consumption. 2 Publications1
Mutagenesisi177H → A: No effect. 1 Publication1
Mutagenesisi178H → A, E or N: Abolishes cyanide-resistant oxygen consumption. 2 Publications1
Mutagenesisi224E → A: No effect. 1 Publication1
Mutagenesisi227E → A, D or H: Abolishes cyanide-resistant oxygen consumption. 2 Publications1
Mutagenesisi296E → A, D or H: Abolishes cyanide-resistant oxygen consumption. 2 Publications1
Mutagenesisi298E → A: No effect. 1 Publication1
Mutagenesisi299H → A, E or N: Abolishes cyanide-resistant oxygen consumption. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 56Chloroplast and chromoplastSequence analysisAdd BLAST56
ChainiPRO_000004542357 – 351Ubiquinol oxidase 4, chloroplastic/chromoplasticAdd BLAST295

Proteomic databases

PaxDbiQ56X52

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Developmental stagei

Expressed throughout the development of the leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ56X52 baseline and differential
GenevisibleiQ56X52 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G22260.1

Structurei

3D structure databases

ProteinModelPortaliQ56X52
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi90 – 95Poly-Ser6

Sequence similaritiesi

Belongs to the alternative oxidase family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II7R Eukaryota
ENOG410YEK7 LUCA
HOGENOMiHOG000023024
InParanoidiQ56X52
KOiK17893
OMAiVESHAYE
OrthoDBiEOG09360FDK
PhylomeDBiQ56X52

Family and domain databases

CDDicd01053 AOX, 1 hit
Gene3Di1.20.1260.140, 1 hit
InterProiView protein in InterPro
IPR002680 AOX
IPR038659 AOX_sf
PANTHERiPTHR31803 PTHR31803, 1 hit
PfamiView protein in Pfam
PF01786 AOX, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56X52-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAISGISSG TLTISRPLVT LRRSRAAVSY SSSHRLLHHL PLSSRRLLLR
60 70 80 90 100
NNHRVQATIL QDDEEKVVVE ESFKAETSTG TEPLEEPNMS SSSTSAFETW
110 120 130 140 150
IIKLEQGVNV FLTDSVIKIL DTLYRDRTYA RFFVLETIAR VPYFAFMSVL
160 170 180 190 200
HMYETFGWWR RADYLKVHFA ESWNEMHHLL IMEELGGNSW WFDRFLAQHI
210 220 230 240 250
ATFYYFMTVF LYILSPRMAY HFSECVESHA YETYDKFLKA SGEELKNMPA
260 270 280 290 300
PDIAVKYYTG GDLYLFDEFQ TSRTPNTRRP VIENLYDVFV NIRDDEAEHC
310 320 330 340 350
KTMRACQTLG SLRSPHSILE DDDTEEESGC VVPEEAHCEG IVDCLKKSIT

S
Length:351
Mass (Da):40,574
Last modified:January 10, 2006 - v2
Checksum:iD8A38C9A4EC0EF69
GO

Sequence cautioni

The sequence BAD94037 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA16776 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79181 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti320E → D in CAA06190 (PubMed:9878632).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098072 mRNA Translation: AAD03599.1
AJ004881 mRNA Translation: CAA06190.1
AL021712 Genomic DNA Translation: CAA16776.1 Sequence problems.
AL161557 Genomic DNA Translation: CAB79181.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE84583.1
AF324663 mRNA Translation: AAG40014.1
AF326898 mRNA Translation: AAG41480.1
AF339717 mRNA Translation: AAK00399.1
AY045699 mRNA Translation: AAK74057.1
BT000558 mRNA Translation: AAN18127.1
AK221825 mRNA Translation: BAD94037.1 Different initiation.
PIRiT04907
T52422
RefSeqiNP_567658.1, NM_118352.4
UniGeneiAt.20480

Genome annotation databases

EnsemblPlantsiAT4G22260.1; AT4G22260.1; AT4G22260
GeneIDi828321
GrameneiAT4G22260.1; AT4G22260.1; AT4G22260
KEGGiath:AT4G22260

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098072 mRNA Translation: AAD03599.1
AJ004881 mRNA Translation: CAA06190.1
AL021712 Genomic DNA Translation: CAA16776.1 Sequence problems.
AL161557 Genomic DNA Translation: CAB79181.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE84583.1
AF324663 mRNA Translation: AAG40014.1
AF326898 mRNA Translation: AAG41480.1
AF339717 mRNA Translation: AAK00399.1
AY045699 mRNA Translation: AAK74057.1
BT000558 mRNA Translation: AAN18127.1
AK221825 mRNA Translation: BAD94037.1 Different initiation.
PIRiT04907
T52422
RefSeqiNP_567658.1, NM_118352.4
UniGeneiAt.20480

3D structure databases

ProteinModelPortaliQ56X52
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G22260.1

Proteomic databases

PaxDbiQ56X52

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G22260.1; AT4G22260.1; AT4G22260
GeneIDi828321
GrameneiAT4G22260.1; AT4G22260.1; AT4G22260
KEGGiath:AT4G22260

Organism-specific databases

AraportiAT4G22260
TAIRilocus:2005514 AT4G22260

Phylogenomic databases

eggNOGiENOG410II7R Eukaryota
ENOG410YEK7 LUCA
HOGENOMiHOG000023024
InParanoidiQ56X52
KOiK17893
OMAiVESHAYE
OrthoDBiEOG09360FDK
PhylomeDBiQ56X52

Miscellaneous databases

PROiPR:Q56X52

Gene expression databases

ExpressionAtlasiQ56X52 baseline and differential
GenevisibleiQ56X52 AT

Family and domain databases

CDDicd01053 AOX, 1 hit
Gene3Di1.20.1260.140, 1 hit
InterProiView protein in InterPro
IPR002680 AOX
IPR038659 AOX_sf
PANTHERiPTHR31803 PTHR31803, 1 hit
PfamiView protein in Pfam
PF01786 AOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAOX4_ARATH
AccessioniPrimary (citable) accession number: Q56X52
Secondary accession number(s): O49631, Q9SBA4, Q9ZSQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: April 25, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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Main funding by: National Institutes of Health

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