UniProtKB - Q56VR3 (FAXC_PSETE)
Venom prothrombin activator pseutarin-C catalytic subunit
Functioni
Snake prothrombin activator that attacks the hemostatic system of prey. This non-catalytic subunit is functionally similar to blood coagulation factor V (PubMed:12362232, PubMed:23869089).
It serves as a critical cofactor for the prothrombinase activity of the catalytic subunit, which is similar to the blood coagulation factor X (PubMed:12362232, PubMed:23869089).
The complex converts prothrombin to thrombin by sequential cleavage at two positions, Arg-320 followed by Arg-271 (PubMed:23869089).
Cleavage at Arg-320 produces an active intermediate known as meizothrombin (PubMed:23869089).
Meizothrombin is the 'second' substrate for prothrombinase, and it docks in an altered manner to present the second cleavage site (271) (PubMed:23869089).
Cleavage at Arg-271 releases active thrombin from its pro-fragment (PubMed:23869089).
This order of events is reversed if the protease component of prothrombinase is used on its own, suggesting that the 271 site is inherently more accessible to proteolysis (PubMed:23869089).
The complex converts prothrombin to thrombin in presence but also in the absence of membrane (PubMed:23869089).
2 PublicationsMiscellaneous
Catalytic activityi
- Selective cleavage of Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin.2 Publications EC:3.4.21.6
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 251 | Charge relay systemBy similarity | 1 | |
Active sitei | 309 | Charge relay systemBy similarity | 1 | |
Active sitei | 406 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- peptidase activator activity Source: UniProtKB-KW
- serine-type endopeptidase activity Source: UniProtKB
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- blood coagulation Source: InterPro
- envenomation resulting in positive regulation of blood coagulation in other organism Source: UniProtKB
- positive regulation of blood coagulation in other organism Source: UniProtKB
Keywordsi
Molecular function | Blood coagulation cascade activating toxin, Hemostasis impairing toxin, Hydrolase, Protease, Prothrombin activator, Serine protease, Toxin |
Ligand | Calcium |
Enzyme and pathway databases
BRENDAi | 3.4.21.60, 6821 |
Protein family/group databases
MEROPSi | S01.446 |
Names & Taxonomyi
Protein namesi | Recommended name: Venom prothrombin activator pseutarin-C catalytic subunit (EC:3.4.21.62 Publications)Short name: PCCS Short name: vPA Alternative name(s): Venom coagulation factor Xa-like protease Cleaved into the following 2 chains: |
Organismi | Pseudonaja textilis (Eastern brown snake) |
Taxonomic identifieri | 8673 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Elapidae › Acanthophiinae › Pseudonaja |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB
Other locations
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
SecretedPathology & Biotechi
Toxic dosei
Pharmaceutical usei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
PropeptideiPRO_0000408523 | 23 – 40 | 2 PublicationsAdd BLAST | 18 | |
ChainiPRO_5000090539 | 41 – 181 | Pseutarin-C catalytic subunit light chainAdd BLAST | 141 | |
PropeptideiPRO_0000408525 | 182 – 209 | Activation peptideBy similarityAdd BLAST | 28 | |
ChainiPRO_0000408526 | 210 – 467 | Pseutarin-C catalytic subunit heavy chainAdd BLAST | 258 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 46 | 4-carboxyglutamatePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 47 | 4-carboxyglutamatePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 54 | 4-carboxyglutamatePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 56 | 4-carboxyglutamatePROSITE-ProRule annotation1 Publication | 1 | |
Disulfide bondi | 57 ↔ 62 | By similarity | ||
Modified residuei | 59 | 4-carboxyglutamatePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 60 | 4-carboxyglutamatePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 65 | 4-carboxyglutamatePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 66 | 4-carboxyglutamatePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 69 | 4-carboxyglutamatePROSITE-ProRule annotation | 1 | |
Modified residuei | 72 | 4-carboxyglutamatePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 90 ↔ 101 | By similarity | ||
Glycosylationi | 92 | O-linked (Hex...) serine1 Publication | 1 | |
Disulfide bondi | 95 ↔ 110 | By similarity | ||
Disulfide bondi | 112 ↔ 121 | By similarity | ||
Disulfide bondi | 129 ↔ 140 | Combined sources1 Publication | ||
Disulfide bondi | 136 ↔ 149 | Combined sources1 Publication | ||
Disulfide bondi | 151 ↔ 164 | Combined sources1 Publication | ||
Disulfide bondi | 172 ↔ 329 | Interchain (between light and heavy chains)Combined sources1 Publication | ||
Disulfide bondi | 216 ↔ 221 | Combined sources1 Publication | ||
Disulfide bondi | 236 ↔ 252 | Combined sources1 Publication | ||
Glycosylationi | 254 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 377 ↔ 391 | Combined sources1 Publication | ||
Disulfide bondi | 402 ↔ 430 | Combined sources1 Publication |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 75 | Not modifiedCurated | 1 | |
Sitei | 103 | Not modified | 1 | |
Sitei | 209 – 210 | Cleavage | 2 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Gamma-carboxyglutamic acid, GlycoproteinPTM databases
iPTMneti | Q56VR3 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Heterodimer of a light and a heavy chains; disulfide-linked. Is associated with pseutarin-C non-catalytic subunit (AC Q7SZN0) in a non-covalent manner.
3 PublicationsStructurei
Secondary structure
3D structure databases
SMRi | Q56VR3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 86 | GlaPROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 86 – 122 | EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 129 – 164 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 210 – 454 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 245 |
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Repeat, SignalFamily and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 4.10.740.10, 1 hit |
InterProi | View protein in InterPro IPR017857, Coagulation_fac-like_Gla_dom IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR035972, GLA-like_dom_SF IPR000294, GLA_domain IPR009030, Growth_fac_rcpt_cys_sf IPR012224, Pept_S1A_FX IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00594, Gla, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001143, Factor_X, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN PR00001, GLABLOOD |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00179, EGF_CA, 1 hit SM00069, GLA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57184, SSF57184, 1 hit SSF57630, SSF57630, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 2 hits PS50026, EGF_3, 1 hit PS01187, EGF_CA, 1 hit PS00011, GLA_1, 1 hit PS50998, GLA_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAPQLLLCLI LTFLWSLPEA ESNVFLKSKV ANRFLQRTKR ANSLVEEFKS
60 70 80 90 100
GNIERECIEE RCSKEEAREV FEDDEKTETF WNVYVDGDQC SSNPCHYRGI
110 120 130 140 150
CKDGIGSYTC TCLSGYEGKN CERVLYKSCR VDNGNCWHFC KSVQNDIQCS
160 170 180 190 200
CAEGYLLGED GHSCVAGGNF SCGRNIKTRN KREASLPDFV QSHNATLLKK
210 220 230 240 250
SDNPSPDIRI VNGMDCKLGE CPWQAALVDD KKGVFCGGTI LSPIYVLTAA
260 270 280 290 300
HCINETETIS VVVGEIDRSR AETGPLLSVD KVYVHKKFVP PKKSQEFYEK
310 320 330 340 350
FDLVSYDYDI AIIQMKTPIQ FSENVVPACL PTADFANQVL MKQDFGIVSG
360 370 380 390 400
FGGIFERGPN SKTLKVLKVP YVDRHTCMLS SNFPITPTMF CAGYDTLPQD
410 420 430 440 450
ACQGDSGGPH ITAYRDTHFI TGIVSWGEGC ARKGRYGIYT KLSKFIPWIK
460
RIMRQKLPST ESSTGRL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 39 | K → E in AAT42490 (PubMed:23869089).Curated | 1 | |
Sequence conflicti | 70 | V → A in AAP86642 (PubMed:15351847).Curated | 1 | |
Sequence conflicti | 142 | S → H in AAT42490 (PubMed:23869089).Curated | 1 | |
Sequence conflicti | 185 | S → N in AAT42490 (PubMed:23869089).Curated | 1 | |
Sequence conflicti | 193 | H → Q in AAP86642 (PubMed:15351847).Curated | 1 | |
Sequence conflicti | 193 | H → Q in AAT42490 (PubMed:23869089).Curated | 1 | |
Sequence conflicti | 196 | T → P in AAP86642 (PubMed:15351847).Curated | 1 | |
Sequence conflicti | 200 | K → I in AAP86642 (PubMed:15351847).Curated | 1 | |
Sequence conflicti | 230 – 232 | DKK → EKE in AAT42490 (PubMed:23869089).Curated | 3 | |
Sequence conflicti | 268 – 271 | RSRA → KSRI in AAT42490 (PubMed:23869089).Curated | 4 | |
Sequence conflicti | 282 | V → I in AAT42490 (PubMed:23869089).Curated | 1 | |
Sequence conflicti | 292 – 305 | KKSQE…FDLVS → QKAYKFDLAA in AAT42490 (PubMed:23869089).CuratedAdd BLAST | 14 | |
Sequence conflicti | 353 – 360 | GIFERGPN → RIVEKGPK in AAT42490 (PubMed:23869089).Curated | 8 | |
Sequence conflicti | 379 – 388 | LSSNFPITPT → VSSETPITPN in AAT42490 (PubMed:23869089).Curated | 10 | |
Sequence conflicti | 399 | Q → R in AAT42490 (PubMed:23869089).Curated | 1 | |
Sequence conflicti | 411 – 413 | ITA → TTV in AAT42490 (PubMed:23869089).Curated | 3 | |
Sequence conflicti | 426 | W → S in AAT42490 (PubMed:23869089).Curated | 1 | |
Sequence conflicti | 433 – 438 | KGRYGI → NGKYGN in AAT42490 (PubMed:23869089).Curated | 6 | |
Sequence conflicti | 450 – 467 | Missing in AAP86642 (PubMed:15351847).CuratedAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY260939 mRNA Translation: AAP86642.1 AY631239 mRNA Translation: AAT42491.1 DQ533835 Genomic DNA Translation: ABG02407.1 AY631238 mRNA Translation: AAT42490.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY260939 mRNA Translation: AAP86642.1 AY631239 mRNA Translation: AAT42491.1 DQ533835 Genomic DNA Translation: ABG02407.1 AY631238 mRNA Translation: AAT42490.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4BXS | X-ray | 3.32 | A | 41-463 | [»] | |
4BXW | X-ray | 2.71 | A/B | 41-463 | [»] | |
SMRi | Q56VR3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
MEROPSi | S01.446 |
PTM databases
iPTMneti | Q56VR3 |
Enzyme and pathway databases
BRENDAi | 3.4.21.60, 6821 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 4.10.740.10, 1 hit |
InterProi | View protein in InterPro IPR017857, Coagulation_fac-like_Gla_dom IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR035972, GLA-like_dom_SF IPR000294, GLA_domain IPR009030, Growth_fac_rcpt_cys_sf IPR012224, Pept_S1A_FX IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00594, Gla, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001143, Factor_X, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN PR00001, GLABLOOD |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00179, EGF_CA, 1 hit SM00069, GLA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57184, SSF57184, 1 hit SSF57630, SSF57630, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 2 hits PS50026, EGF_3, 1 hit PS01187, EGF_CA, 1 hit PS00011, GLA_1, 1 hit PS50998, GLA_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FAXC_PSETE | |
Accessioni | Q56VR3Primary (citable) accession number: Q56VR3 Secondary accession number(s): A5X463, Q6IT09, Q6IT10 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 31, 2011 |
Last sequence update: | June 28, 2011 | |
Last modified: | June 2, 2021 | |
This is version 109 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Pharmaceutical, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families