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Protein

Charged multivesicular body protein 4c

Gene

Chmp4c

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: upon phosphorylation by AURKB, together with ZFYVE19/ANCHR, retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ANCHR and VPS4 and subsequent abscission. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 4c
Alternative name(s):
Chromatin-modifying protein 4c
Short name:
CHMP4c
Gene namesi
Name:Chmp4c
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1564543 Chmp4c

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002114971 – 232Charged multivesicular body protein 4cAdd BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei210Phosphoserine; by AURKBBy similarity1

Post-translational modificationi

Phosphorylated at Ser-210 by AURKB during cytokinesis: together with ZFYVE19/ANCHR, phosphorylated CHMP4C retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ569C1
PRIDEiQ569C1

Interactioni

Subunit structurei

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. Self-associates. Interacts with CHMP2A. Interacts with CHMP4A. Interacts with CHMP4B. Interacts with CHMP6. Interacts with VPS4A. Interacts with PDCD6IP; the interaction is direct (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013720

Structurei

3D structure databases

ProteinModelPortaliQ569C1
SMRiQ569C1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 153Intramolecular interaction with C-terminusBy similarityAdd BLAST153
Regioni154 – 232Intramolecular interaction with N-terminusBy similarityAdd BLAST79

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili21 – 50Sequence analysisAdd BLAST30
Coiled coili125 – 182Sequence analysisAdd BLAST58

Domaini

The acidic C-terminus and the basic N-terminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components (By similarity).By similarity

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1656 Eukaryota
ENOG410YE9I LUCA
HOGENOMiHOG000209959
HOVERGENiHBG050928
InParanoidiQ569C1
KOiK12194
PhylomeDBiQ569C1

Family and domain databases

InterProiView protein in InterPro
IPR005024 Snf7_fam
PfamiView protein in Pfam
PF03357 Snf7, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q569C1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKLGKFFKG SRSSRARAAP SAQEALARLR EIEEMMAKKQ EYLENRIQRE
60 70 80 90 100
LALAKKHGSQ NKRAALQALK RKKRFEKQLT QIDGTLSTIE FQREALENSH
110 120 130 140 150
TNTEVLRNMG FAAKAMKAVH ENMDLNKIDD LMQDITEQQD IAQEISEAFS
160 170 180 190 200
QRVQFADGFD EDELLAELEE LEQEELNKKM TSMELPNVPS SSLPAQPSRK
210 220 230
AGVPSSVHRS RAASSRRAEE DDDFKQLAAW AT
Length:232
Mass (Da):26,324
Last modified:May 10, 2005 - v1
Checksum:iCE924438BF9264AE
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1LPT7F1LPT7_RAT
Charged multivesicular body protein...
Chmp4c
232Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092576 mRNA Translation: AAH92576.1
RefSeqiNP_001017466.2, NM_001017466.2
UniGeneiRn.162567

Genome annotation databases

GeneIDi361916
KEGGirno:361916
UCSCiRGD:1564543 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092576 mRNA Translation: AAH92576.1
RefSeqiNP_001017466.2, NM_001017466.2
UniGeneiRn.162567

3D structure databases

ProteinModelPortaliQ569C1
SMRiQ569C1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013720

Proteomic databases

PaxDbiQ569C1
PRIDEiQ569C1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi361916
KEGGirno:361916
UCSCiRGD:1564543 rat

Organism-specific databases

CTDi92421
RGDi1564543 Chmp4c

Phylogenomic databases

eggNOGiKOG1656 Eukaryota
ENOG410YE9I LUCA
HOGENOMiHOG000209959
HOVERGENiHBG050928
InParanoidiQ569C1
KOiK12194
PhylomeDBiQ569C1

Miscellaneous databases

PROiPR:Q569C1

Family and domain databases

InterProiView protein in InterPro
IPR005024 Snf7_fam
PfamiView protein in Pfam
PF03357 Snf7, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHM4C_RAT
AccessioniPrimary (citable) accession number: Q569C1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: May 10, 2005
Last modified: June 20, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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