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Protein

Microbial collagenase

Gene

prt

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses gelatinolytic activity. Can cause weak haemolysis on blood agar.

Catalytic activityi

Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1'; Pro and Ala at P2 and P2'; and hydroxyproline, Ala or Arg at P3'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi435Zinc; catalyticPROSITE-ProRule annotation1
Active sitei436PROSITE-ProRule annotation1
Metal bindingi439Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processCollagen degradation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciVPAR223926:G1GTB-3690-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Microbial collagenase (EC:3.4.24.3)
Alternative name(s):
prtVp
Gene namesi
Name:prt
Ordered Locus Names:VPA0459
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000002867928 – 816Microbial collagenaseAdd BLAST789

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiQ56696

Interactioni

Protein-protein interaction databases

STRINGi223926.VPA0459

Structurei

3D structure databases

SMRiQ56696
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M9A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105KXC Bacteria
ENOG410XSXR LUCA
HOGENOMiHOG000218161
KOiK01387
OMAiRYAAVYV

Family and domain databases

InterProiView protein in InterPro
IPR007280 Peptidase_C_arc/bac
IPR013661 Peptidase_M9_N_dom
IPR002169 Peptidase_M9A/M9B
PfamiView protein in Pfam
PF01752 Peptidase_M9, 1 hit
PF08453 Peptidase_M9_N, 1 hit
PF04151 PPC, 1 hit
PRINTSiPR00931 MICOLLPTASE
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56696-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSHIRFFPRH RLALACMLAS VSSFSFAQNQ CAVADLQQSR DLAAAVSGAE
60 70 80 90 100
YDCYHAWFSA PSATLNDIYS EASLSRIQVA LDQEIARYRG EAEQARVLEN
110 120 130 140 150
LGEFVRAAYY VRYNAGTGTP EFSEALSQRF AQSTNLFLNN PHALDQGREQ
160 170 180 190 200
VGAMKSLTLM VDNVKQLPLT MDSMMAALMH FNRDTAKDTQ WVDGLNNLFR
210 220 230 240 250
SMAGHAANDA FYRYMANNTH HIDTLARFAS DNAWALDTDA NFIVFNALRE
260 270 280 290 300
TGRLLASPDQ ETKRKALAVM QQVMQRYPLG SEHDKLWLAA VEMMSYYAPE
310 320 330 340 350
GLNGLNLEQA KQDLAARVMP NRFECQGPAI IRSEDLTDAQ AAKACEVLAA
360 370 380 390 400
KEADFHQVAN TGNQPVADDL NDRVEVAVFA SNDSYVDYSS FLFGNTTDNG
410 420 430 440 450
GQYLEGTPSR ADNTARFVAY RYANGEDLSI LNLEHEYTHY LDARFNQYGS
460 470 480 490 500
FSDNLAHGHI VWWLEGFAEY MHYKQGYKAA IDLIPSGKLS LSTVFDTTYS
510 520 530 540 550
HDSNRIYRWG YLAVRFMLEN HPQDVESLLA LSRSGQFAQW AQQVTVLGQQ
560 570 580 590 600
YDAEFERWLD TLEVVVEPEQ PGTDPEEPSE PTDPEVQVTE LAANQSLQLS
610 620 630 640 650
GEAYSEKLFY VDVPANTVRF NVSIEGAGDA DLYMSYNKVA HYYDFEMSQY
660 670 680 690 700
ADGSNEEIQF APEQNGYVKA GRYYISLTGR DSYDSVNLVA ALEVEAQTPP
710 720 730 740 750
TQVQDDLAPV VLESGEAKVL TVHQQRYAAV YVPEGVKEVR VWMSSQSNAN
760 770 780 790 800
DPYGAGNVDL YASRKHWPTA EQHEYASNYA GSNEYLAIPV TEAGYVHFSL
810
QAPQQGDDVE MLVYFF
Length:816
Mass (Da):91,444
Last modified:April 11, 2003 - v2
Checksum:i25AB529725C6F6CB
GO

Sequence cautioni

The sequence CAA86734 differs from that shown. Reason: Frameshift at position 582.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti175M → I in CAA86734 (PubMed:7582017).Curated1
Sequence conflicti311 – 312KQ → NE in CAA86734 (PubMed:7582017).Curated2
Sequence conflicti337T → S in CAA86734 (PubMed:7582017).Curated1
Sequence conflicti542 – 543QQ → HE in CAA86734 (PubMed:7582017).Curated2
Sequence conflicti580E → R (PubMed:7582017).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000032 Genomic DNA Translation: BAC61802.1
Z46782 Genomic DNA Translation: CAA86734.1 Frameshift.
RefSeqiNP_799969.1, NC_004605.1
WP_005479219.1, NC_004605.1

Genome annotation databases

EnsemblBacteriaiBAC61802; BAC61802; BAC61802
GeneIDi1191147
KEGGivpa:VPA0459
PATRICifig|223926.6.peg.3400

Similar proteinsi

Entry informationi

Entry nameiCOLA_VIBPA
AccessioniPrimary (citable) accession number: Q56696
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 11, 2003
Last modified: May 23, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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