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Protein

Serine/threonine-protein phosphatase PGAM5, mitochondrial

Gene

Pgam5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression (By similarity). Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNecrosis

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PGAM5, mitochondrial (EC:3.1.3.16)
Alternative name(s):
Phosphoglycerate mutase family member 5
Gene namesi
Name:Pgam5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1312028 Pgam5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 29HelicalSequence analysisAdd BLAST23

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002887841 – 288Serine/threonine-protein phosphatase PGAM5, mitochondrialAdd BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86PhosphoserineBy similarity1
Modified residuei115N6-acetyllysineBy similarity1
Modified residuei143N6-acetyllysineBy similarity1
Modified residuei190N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ562B5
PRIDEiQ562B5

PTM databases

iPTMnetiQ562B5
PhosphoSitePlusiQ562B5

Interactioni

Subunit structurei

Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5 (By similarity). Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation (By similarity). Interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation (By similarity).By similarity

Protein-protein interaction databases

BioGridi252666, 1 interactor
STRINGi10116.ENSRNOP00000053513

Structurei

3D structure databases

ProteinModelPortaliQ562B5
SMRiQ562B5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 81Interaction with KEAP1By similarity6

Domaini

The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4609 Eukaryota
ENOG410XXCQ LUCA
HOGENOMiHOG000261217
HOVERGENiHBG105576
InParanoidiQ562B5
KOiK15637
PhylomeDBiQ562B5

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
PfamiView protein in Pfam
PF00300 His_Phos_1, 1 hit
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF53254 SSF53254, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q562B5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRAGGDADTR TTEPLAWTGA
60 70 80 90 100
RPGHGVWDSN WDRREPLSLI NLKKRNVEFG EDELASRLDH YKAKATRHIF
110 120 130 140 150
LIRHSQYNVD GSMEKDRTLT PLGREQAELT GIRLASLGLK FNKIVHSSMT
160 170 180 190 200
RAVETTDIIS KHLPGVCRVS TDLLREGAPI EPDPPVSHWK PEAVQYYEDG
210 220 230 240 250
ARIEAAFRNY IHRADAKQEE DSYEIFICHA NVIRYIVCRA LQFPPEGWLR
260 270 280
LSLNNGSITH LVIRPNGRVA LRTLGDTGFM PPDKITRS
Length:288
Mass (Da):32,060
Last modified:May 10, 2005 - v1
Checksum:iB4DD3F3D276A61C5
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V9N1G3V9N1_RAT
RCG21137
Pgam5 rCG_21137
288Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092607 mRNA Translation: AAH92607.1
RefSeqiNP_001020443.1, NM_001025272.1
UniGeneiRn.23489

Genome annotation databases

GeneIDi288731
KEGGirno:288731
UCSCiRGD:1312028 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092607 mRNA Translation: AAH92607.1
RefSeqiNP_001020443.1, NM_001025272.1
UniGeneiRn.23489

3D structure databases

ProteinModelPortaliQ562B5
SMRiQ562B5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi252666, 1 interactor
STRINGi10116.ENSRNOP00000053513

PTM databases

iPTMnetiQ562B5
PhosphoSitePlusiQ562B5

Proteomic databases

PaxDbiQ562B5
PRIDEiQ562B5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi288731
KEGGirno:288731
UCSCiRGD:1312028 rat

Organism-specific databases

CTDi192111
RGDi1312028 Pgam5

Phylogenomic databases

eggNOGiKOG4609 Eukaryota
ENOG410XXCQ LUCA
HOGENOMiHOG000261217
HOVERGENiHBG105576
InParanoidiQ562B5
KOiK15637
PhylomeDBiQ562B5

Miscellaneous databases

PROiPR:Q562B5

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
PfamiView protein in Pfam
PF00300 His_Phos_1, 1 hit
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF53254 SSF53254, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPGAM5_RAT
AccessioniPrimary (citable) accession number: Q562B5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 10, 2005
Last modified: September 12, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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