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UniProtKB - Q56185 (HPPE_STRWE)
Protein
(S)-2-hydroxypropylphosphonic acid epoxidase
Gene
hppE
Organism
Streptomyces wedmorensis
Status
Functioni
Non-heme-dependent dioxygenase that catalyzes the oxidative epoxidation of (S)-2-hydroxypropylphosphonate into (1R,2S)-epoxypropylphosphonate, the final step in the biosynthesis of fosfomycin antibiotic.
2 PublicationsMiscellaneous
Phosphinothricin tripeptide (PTT) herbicide and fosfomycin antibiotic biosynthesis pathways share early steps starting with phosphoenolpyruvate before the pathways diverge after formation of 2-hydroxyethylphosphonate (HEP). HppE is involved in fosfomycin biosynthesis after divergence of the 2 pathways.1 Publication
Catalytic activityi
- EC:1.11.1.231 Publication
Cofactori
Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication
: fosfomycin biosynthesis Pathwayi
This protein is involved in the pathway fosfomycin biosynthesis, which is part of Antibiotic biosynthesis.2 PublicationsView all proteins of this organism that are known to be involved in the pathway fosfomycin biosynthesis and in Antibiotic biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 23 | SubstrateCombined sources2 Publications | 1 | |
Binding sitei | 97 | SubstrateCombined sources3 Publications | 1 | |
Binding sitei | 105 | SubstrateCombined sources3 Publications | 1 | |
Metal bindingi | 138 | Iron; via tele nitrogenCombined sources3 Publications | 1 | |
Metal bindingi | 142 | IronCombined sources3 Publications | 1 | |
Binding sitei | 142 | SubstrateCombined sources3 Publications | 1 | |
Metal bindingi | 180 | Iron; via tele nitrogenCombined sources3 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 26 – 45 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- dioxygenase activity Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- ferrous iron binding Source: UniProtKB
- oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water Source: UniProtKB
GO - Biological processi
- antibiotic biosynthetic process Source: UniProtKB-KW
- phosphinothricin biosynthetic process Source: UniProtKB
- protein homotetramerization Source: UniProtKB
Keywordsi
Molecular function | Dioxygenase, DNA-binding, Oxidoreductase |
Biological process | Antibiotic biosynthesis |
Ligand | Iron, Metal-binding, NAD |
Enzyme and pathway databases
BRENDAi | 1.11.1.23, 6118 1.14.19.7, 6118 |
UniPathwayi | UPA01071 |
Names & Taxonomyi
Protein namesi | Recommended name: (S)-2-hydroxypropylphosphonic acid epoxidase (EC:1.11.1.231 Publication)Alternative name(s): Fosfomycin biosynthesis protein 4 Hydroxypropylphosphonate epoxidase Short name: Sw-hppE |
Gene namesi | Name:hppE Synonyms:fom4 |
Organismi | Streptomyces wedmorensis |
Taxonomic identifieri | 43759 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 23 | K → A: Abolishes (S)-2-hydroxypropylphosphonic acid epoxidase activity. 1 Publication | 1 | |
Mutagenesisi | 105 | Y → F: Abolishes (S)-2-hydroxypropylphosphonic acid epoxidase activity. 1 Publication | 1 | |
Mutagenesisi | 142 | E → A: Abolishes (S)-2-hydroxypropylphosphonic acid epoxidase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000422030 | 1 – 198 | (S)-2-hydroxypropylphosphonic acid epoxidaseAdd BLAST | 198 |
Interactioni
Subunit structurei
Homotetramer.
3 PublicationsStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q56185 |
SMRi | Q56185 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q56185 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 15 – 70 | HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST | 56 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 135 – 138 | Substrate bindingCombined sources3 Publications | 4 |
Sequence similaritiesi
Belongs to the non-heme iron-dependent dioxygenase family.Curated
Family and domain databases
CDDi | cd00093, HTH_XRE, 1 hit |
Gene3Di | 1.10.260.40, 1 hit 2.60.120.10, 1 hit |
InterProi | View protein in InterPro IPR001387, Cro/C1-type_HTH IPR013096, Cupin_2 IPR010982, Lambda_DNA-bd_dom_sf IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF07883, Cupin_2, 1 hit PF01381, HTH_3, 1 hit |
SMARTi | View protein in SMART SM00530, HTH_XRE, 1 hit |
SUPFAMi | SSF47413, SSF47413, 1 hit SSF51182, SSF51182, 1 hit |
PROSITEi | View protein in PROSITE PS50943, HTH_CROC1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q56185-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNTKTASTG FAELLKDRRE QVKMDHAALA SLLGETPETV AAWENGEGGE
60 70 80 90 100
LTLTQLGRIA HVLGTSIGAL TPPAGNDLDD GVIIQMPDER PILKGVRDNV
110 120 130 140 150
DYYVYNCLVR TKRAPSLVPL VVDVLTDNPD DAKFNSGHAG NEFLFVLEGE
160 170 180 190
IHMKWGDKEN PKEALLPTGA SMFVEEHVPH AFTAAKGTGS AKLIAVNF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB016934 Genomic DNA Translation: BAA32491.1 |
PIRi | S60215 |
RefSeqi | WP_033205181.1, NZ_JNWK01000026.1 |
Genome annotation databases
KEGGi | ag:BAA32491 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB016934 Genomic DNA Translation: BAA32491.1 |
PIRi | S60215 |
RefSeqi | WP_033205181.1, NZ_JNWK01000026.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZZ6 | X-ray | 2.00 | A/B | 1-198 | [»] | |
1ZZ7 | X-ray | 2.10 | A/B | 1-198 | [»] | |
1ZZ8 | X-ray | 2.30 | A/B/C | 1-198 | [»] | |
1ZZ9 | X-ray | 2.40 | A/B/C | 1-198 | [»] | |
1ZZB | X-ray | 2.30 | A/B | 1-198 | [»] | |
1ZZC | X-ray | 1.80 | A/B | 1-198 | [»] | |
2BNM | X-ray | 1.70 | A/B | 1-198 | [»] | |
2BNN | X-ray | 2.50 | A/B | 1-198 | [»] | |
2BNO | X-ray | 1.90 | A/B | 1-198 | [»] | |
3SCF | X-ray | 2.85 | A/B/C | 1-198 | [»] | |
3SCG | X-ray | 3.00 | A/B/C | 1-198 | [»] | |
3SCH | X-ray | 2.10 | A/B | 1-198 | [»] | |
4J1W | X-ray | 2.72 | A/B/C | 1-198 | [»] | |
4J1X | X-ray | 2.80 | A/B/C | 2-198 | [»] | |
AlphaFoldDBi | Q56185 | |||||
SMRi | Q56185 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
KEGGi | ag:BAA32491 |
Enzyme and pathway databases
UniPathwayi | UPA01071 |
BRENDAi | 1.11.1.23, 6118 1.14.19.7, 6118 |
Miscellaneous databases
EvolutionaryTracei | Q56185 |
Family and domain databases
CDDi | cd00093, HTH_XRE, 1 hit |
Gene3Di | 1.10.260.40, 1 hit 2.60.120.10, 1 hit |
InterProi | View protein in InterPro IPR001387, Cro/C1-type_HTH IPR013096, Cupin_2 IPR010982, Lambda_DNA-bd_dom_sf IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF07883, Cupin_2, 1 hit PF01381, HTH_3, 1 hit |
SMARTi | View protein in SMART SM00530, HTH_XRE, 1 hit |
SUPFAMi | SSF47413, SSF47413, 1 hit SSF51182, SSF51182, 1 hit |
PROSITEi | View protein in PROSITE PS50943, HTH_CROC1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HPPE_STRWE | |
Accessioni | Q56185Primary (citable) accession number: Q56185 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2013 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families