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Protein

(S)-2-hydroxypropylphosphonic acid epoxidase

Gene

hppE

Organism
Streptomyces wedmorensis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-heme-dependent dioxygenase that catalyzes the oxidative epoxidation of (S)-2-hydroxypropylphosphonate into (1R,2S)-epoxypropylphosphonate, the final step in the biosynthesis of fosfomycin antibiotic.2 Publications

Miscellaneous

Phosphinothricin tripeptide (PTT) herbicide and fosfomycin antibiotic biosynthesis pathways share early steps starting with phosphoenolpyruvate before the pathways diverge after formation of 2-hydroxyethylphosphonate (HEP). HppE is involved in fosfomycin biosynthesis after divergence of the 2 pathways.1 Publication

Catalytic activityi

(S)-2-hydroxypropylphosphonate + H2O2 = (1R,2S)-1,2-epoxypropylphosphonate + 2 H2O.1 Publication

Cofactori

Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication

Pathwayi: fosfomycin biosynthesis

This protein is involved in the pathway fosfomycin biosynthesis, which is part of Antibiotic biosynthesis.2 Publications
View all proteins of this organism that are known to be involved in the pathway fosfomycin biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23SubstrateCombined sources2 Publications1
Binding sitei97SubstrateCombined sources3 Publications1
Binding sitei105SubstrateCombined sources3 Publications1
Metal bindingi138Iron; via tele nitrogenCombined sources3 Publications1
Metal bindingi142IronCombined sources3 Publications1
Binding sitei142SubstrateCombined sources3 Publications1
Metal bindingi180Iron; via tele nitrogenCombined sources3 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi26 – 45H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, DNA-binding, Oxidoreductase
Biological processAntibiotic biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13661
BRENDAi1.11.1.23 6118
1.14.19.7 6118
UniPathwayi
UPA01071

Names & Taxonomyi

Protein namesi
Recommended name:
(S)-2-hydroxypropylphosphonic acid epoxidase (EC:1.11.1.231 Publication)
Alternative name(s):
Fosfomycin biosynthesis protein 4
Hydroxypropylphosphonate epoxidase
Short name:
Sw-hppE
Gene namesi
Name:hppE
Synonyms:fom4
OrganismiStreptomyces wedmorensis
Taxonomic identifieri43759 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23K → A: Abolishes (S)-2-hydroxypropylphosphonic acid epoxidase activity. 1 Publication1
Mutagenesisi105Y → F: Abolishes (S)-2-hydroxypropylphosphonic acid epoxidase activity. 1 Publication1
Mutagenesisi142E → A: Abolishes (S)-2-hydroxypropylphosphonic acid epoxidase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004220301 – 198(S)-2-hydroxypropylphosphonic acid epoxidaseAdd BLAST198

Interactioni

Subunit structurei

Homotetramer.3 Publications

Structurei

Secondary structure

1198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ56185
SMRiQ56185
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56185

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 70HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST56

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 138Substrate bindingCombined sources3 Publications4

Sequence similaritiesi

Phylogenomic databases

KOiK12903

Family and domain databases

CDDicd00093 HTH_XRE, 1 hit
Gene3Di2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR001387 Cro/C1-type_HTH
IPR013096 Cupin_2
IPR010982 Lambda_DNA-bd_dom_sf
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PfamiView protein in Pfam
PF07883 Cupin_2, 1 hit
PF01381 HTH_3, 1 hit
SMARTiView protein in SMART
SM00530 HTH_XRE, 1 hit
SUPFAMiSSF47413 SSF47413, 1 hit
SSF51182 SSF51182, 1 hit
PROSITEiView protein in PROSITE
PS50943 HTH_CROC1, 1 hit

Sequencei

Sequence statusi: Complete.

Q56185-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSNTKTASTG FAELLKDRRE QVKMDHAALA SLLGETPETV AAWENGEGGE
60 70 80 90 100
LTLTQLGRIA HVLGTSIGAL TPPAGNDLDD GVIIQMPDER PILKGVRDNV
110 120 130 140 150
DYYVYNCLVR TKRAPSLVPL VVDVLTDNPD DAKFNSGHAG NEFLFVLEGE
160 170 180 190
IHMKWGDKEN PKEALLPTGA SMFVEEHVPH AFTAAKGTGS AKLIAVNF
Length:198
Mass (Da):21,337
Last modified:November 1, 1996 - v1
Checksum:i550D70A1D0D2FC56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016934 Genomic DNA Translation: BAA32491.1
PIRiS60215
RefSeqiWP_033205181.1, NZ_JNWK01000026.1

Genome annotation databases

KEGGiag:BAA32491

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016934 Genomic DNA Translation: BAA32491.1
PIRiS60215
RefSeqiWP_033205181.1, NZ_JNWK01000026.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZZ6X-ray2.00A/B1-198[»]
1ZZ7X-ray2.10A/B1-198[»]
1ZZ8X-ray2.30A/B/C1-198[»]
1ZZ9X-ray2.40A/B/C1-198[»]
1ZZBX-ray2.30A/B1-198[»]
1ZZCX-ray1.80A/B1-198[»]
2BNMX-ray1.70A/B1-198[»]
2BNNX-ray2.50A/B1-198[»]
2BNOX-ray1.90A/B1-198[»]
3SCFX-ray2.85A/B/C1-198[»]
3SCGX-ray3.00A/B/C1-198[»]
3SCHX-ray2.10A/B1-198[»]
4J1WX-ray2.72A/B/C1-198[»]
4J1XX-ray2.80A/B/C2-198[»]
ProteinModelPortaliQ56185
SMRiQ56185
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAA32491

Phylogenomic databases

KOiK12903

Enzyme and pathway databases

UniPathwayi
UPA01071

BioCyciMetaCyc:MONOMER-13661
BRENDAi1.11.1.23 6118
1.14.19.7 6118

Miscellaneous databases

EvolutionaryTraceiQ56185

Family and domain databases

CDDicd00093 HTH_XRE, 1 hit
Gene3Di2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR001387 Cro/C1-type_HTH
IPR013096 Cupin_2
IPR010982 Lambda_DNA-bd_dom_sf
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PfamiView protein in Pfam
PF07883 Cupin_2, 1 hit
PF01381 HTH_3, 1 hit
SMARTiView protein in SMART
SM00530 HTH_XRE, 1 hit
SUPFAMiSSF47413 SSF47413, 1 hit
SSF51182 SSF51182, 1 hit
PROSITEiView protein in PROSITE
PS50943 HTH_CROC1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHPPE_STRWE
AccessioniPrimary (citable) accession number: Q56185
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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