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Protein

Secreted effector protein SifA

Gene

sifA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and formation of Salmonella-induced filaments (Sifs), which are filamentous structures containing lysosomal membrane glycoproteins within epithelial cells. Sif formation is concomitant with intracellular bacterial replication.3 Publications

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • regulation of protein localization Source: AgBase

Keywordsi

Biological processVirulence

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-1239-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted effector protein SifA
Gene namesi
Name:sifA
Ordered Locus Names:STM1224
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Secreted
  • Host cytoplasm
  • Host cell membrane
  • Note: Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. It associates with membranes and colocalizes with microtubules along the length of Sifs in infected cells.

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host cytoplasm, Host membrane, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene do not produce Sifs in epithelial cells and show attenuated virulence in mice.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi130L → D: Loss of interaction with host PLEKHM2. Fails to induce host endosomal tubulation; when associated with D-131. 1 Publication1
Mutagenesisi131M → D: Alters interaction with host PLEKHM2. Fails to induce host endosomal tubulation; when associated with D-130. 1 Publication1
Mutagenesisi197W → A: Fails to induce host endosomal tubulation; when associated with A-201. 1 Publication1
Mutagenesisi201E → A: Fails to induce host endosomal tubulation; when associated with A-197. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000977601 – 336Secreted effector protein SifAAdd BLAST336

Proteomic databases

PaxDbiQ56061
PRIDEiQ56061

Interactioni

Subunit structurei

Interacts with host PLEKHM2. Interacts with SseJ; the interaction is indirect.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PLEKHM2Q8IWE53EBI-10765408,EBI-726484From Homo sapiens.

Protein-protein interaction databases

DIPiDIP-46411N
IntActiQ56061, 2 interactors
STRINGi99287.STM1224

Structurei

Secondary structure

1336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiQ56061
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56061

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 330Interaction with host PLEKHM2Add BLAST330

Domaini

Domains throughout the protein, and not only the N-terminus, are required for secretion and translocation. Both N- and C-terminal domains are also required for formation of tubules.1 Publication

Sequence similaritiesi

Belongs to the Sif family.Curated

Phylogenomic databases

HOGENOMiHOG000028279
KOiK15347
OMAiLETMTMC

Family and domain databases

InterProiView protein in InterPro
IPR010637 Sif
PfamiView protein in Pfam
PF06767 Sif, 1 hit

Sequencei

Sequence statusi: Complete.

Q56061-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPITIGNGFL KSEILTNSPR NTKEAWWKVL WEKIKDFFFS TGKAKADRCL
60 70 80 90 100
HEMLFAERAP TRERLTEIFF ELKELACASQ RDRFQVHNPH ENDATIILRI
110 120 130 140 150
MDQNEENELL RITQNTDTFS CEVMGNLYFL MKDRPDILKS HPQMTAMIKR
160 170 180 190 200
RYSEIVDYPL PSTLCLNPAG APILSVPLDN IEGYLYTELR KGHLDGWKAQ
210 220 230 240 250
EKATYLAAKI QSGIEKTTRI LHHANISEST QQNAFLETMA MCGLKQLEIP
260 270 280 290 300
PPHTHIPIEK MVKEVLLADK TFQAFLVTDP STSQSMLAEI VEAISDQVFH
310 320 330
AIFRIDPQAI QKMAEEQLTT LHVRSEQQSG CLCCFL
Length:336
Mass (Da):38,498
Last modified:November 1, 1997 - v1
Checksum:i83C6185148A774B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51867 Genomic DNA Translation: AAA97467.1
AE006468 Genomic DNA Translation: AAL20153.1
PIRiS71033
RefSeqiNP_460194.1, NC_003197.2
WP_001520468.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL20153; AAL20153; STM1224
GeneIDi1252742
KEGGistm:STM1224
PATRICifig|99287.12.peg.1294

Similar proteinsi

Entry informationi

Entry nameiSIFA_SALTY
AccessioniPrimary (citable) accession number: Q56061
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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