UniProtKB - Q55FS0 (UBA4_DICDI)
Protein
Adenylyltransferase and sulfurtransferase MOCS3
Gene
mocs3
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and mocs2a. Its N-terminus first activates urm1 and mocs2a as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and mocs2a to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and mocs2a. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions (By similarity).By similarity
Catalytic activityi
- [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H+ = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphateUniRule annotationEC:2.7.7.80UniRule annotation
- [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H+ + L-cysteinyl-[cysteine desulfurase]UniRule annotationEC:2.8.1.11UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis Pathwayi
This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationView all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.
Pathwayi: molybdopterin biosynthesis
This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 47 | ATP; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 68 | ATPUniRule annotation | 1 | |
Binding sitei | 92 | ATPUniRule annotation | 1 | |
Metal bindingi | 177 | ZincUniRule annotation | 1 | |
Metal bindingi | 180 | ZincUniRule annotation | 1 | |
Active sitei | 194 | Glycyl thioester intermediate; for adenylyltransferase activityUniRule annotation | 1 | |
Metal bindingi | 253 | ZincUniRule annotation | 1 | |
Metal bindingi | 256 | ZincUniRule annotation | 1 | |
Active sitei | 378 | Cysteine persulfide intermediate; for sulfurtransferase activityUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 75 – 79 | ATPUniRule annotation | 5 | |
Nucleotide bindingi | 136 – 137 | ATPUniRule annotation | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- molybdopterin-synthase adenylyltransferase activity Source: UniProtKB-EC
- molybdopterin-synthase sulfurtransferase activity Source: UniProtKB-EC
- nucleotidyltransferase activity Source: GO_Central
- thiosulfate sulfurtransferase activity Source: UniProtKB
- URM1 activating enzyme activity Source: GO_Central
GO - Biological processi
- Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB
- tRNA wobble position uridine thiolation Source: InterPro
Keywordsi
Molecular function | Multifunctional enzyme, Transferase |
Biological process | Molybdenum cofactor biosynthesis, tRNA processing |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DDI-947581, Molybdenum cofactor biosynthesis |
UniPathwayi | UPA00344 UPA00988 |
Names & Taxonomyi
Protein namesi | Recommended name: Adenylyltransferase and sulfurtransferase MOCS3UniRule annotationAlternative name(s): Molybdenum cofactor synthesis protein 3UniRule annotation Ubiquitin-like protein activator 4 homologUniRule annotation Including the following 2 domains: Alternative name(s): Adenylyltransferase MOCS3UniRule annotation Sulfur carrier protein MOCS2A adenylyltransferaseUniRule annotation Alternative name(s): Sulfur carrier protein MOCS2A sulfurtransferaseUniRule annotation Sulfurtransferase MOCS3UniRule annotation |
Gene namesi | Name:mocs3 Synonyms:uba4 ORF Names:DDB_G0267980 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0267980, mocs3 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000331263 | 1 – 425 | Adenylyltransferase and sulfurtransferase MOCS3Add BLAST | 425 |
Proteomic databases
PaxDbi | Q55FS0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 305 – 423 | RhodaneseUniRule annotationAdd BLAST | 119 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 304 – 308 | Poly-Asn | 5 | |
Compositional biasi | 355 – 365 | Poly-AsnAdd BLAST | 11 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2017, Eukaryota |
HOGENOMi | CLU_013325_1_2_1 |
InParanoidi | Q55FS0 |
OMAi | GTIGAMQ |
PhylomeDBi | Q55FS0 |
Family and domain databases
Gene3Di | 3.40.250.10, 1 hit |
HAMAPi | MF_03049, MOCS3_Uba4, 1 hit |
InterProi | View protein in InterPro IPR028885, MOCS3/Uba4 IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz |
Pfami | View protein in Pfam PF00581, Rhodanese, 1 hit PF00899, ThiF, 1 hit |
SMARTi | View protein in SMART SM00450, RHOD, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q55FS0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKYIKEHTL KPNEIERYGR QLITPDIGVS GQMSLCNSSV LIIGAGGLGC
60 70 80 90 100
PVALYLSSAG IGTLGLVDYD TVEISNLHRQ IGHRESSKGI SKAVSLSKTI
110 120 130 140 150
SELNSLIKVN TYETTFTSET AMEIIKNYDI VVDASDNVAT RYLVNDACVL
160 170 180 190 200
TGKPLVSGSA LKWEGQITCY NYNNGPCYRC IFPTPPPVET VTKCSDGGVL
210 220 230 240 250
GPIVGVIGSL QALEVIKILT NNKEGVLSGR LLIYDGMSAV FRTVRIRGKQ
260 270 280 290 300
SGCNVCGDKP TVTQLIDYTQ FCQSNYSESA GKVDDRVDKT LIITVEQFNQ
310 320 330 340 350
SIKNNNNNHL LIDVRPKIQF DICSLPNSNN IPIEEIDKKE SIEIIEKLIK
360 370 380 390 400
EKVENNNNNN NNNENRDASD ELSVYLVCRR GNKSQDAVKI LDEKLKEFRD
410 420
KFKLLHIKDG LLGWNESIDD SFPIY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000003 Genomic DNA Translation: EAL73443.1 |
RefSeqi | XP_647463.1, XM_642371.1 |
Genome annotation databases
EnsemblProtistsi | EAL73443; EAL73443; DDB_G0267980 |
GeneIDi | 8616270 |
KEGGi | ddi:DDB_G0267980 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000003 Genomic DNA Translation: EAL73443.1 |
RefSeqi | XP_647463.1, XM_642371.1 |
3D structure databases
SMRi | Q55FS0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 44689.DDB0267172 |
Proteomic databases
PaxDbi | Q55FS0 |
Genome annotation databases
EnsemblProtistsi | EAL73443; EAL73443; DDB_G0267980 |
GeneIDi | 8616270 |
KEGGi | ddi:DDB_G0267980 |
Organism-specific databases
dictyBasei | DDB_G0267980, mocs3 |
Phylogenomic databases
eggNOGi | KOG2017, Eukaryota |
HOGENOMi | CLU_013325_1_2_1 |
InParanoidi | Q55FS0 |
OMAi | GTIGAMQ |
PhylomeDBi | Q55FS0 |
Enzyme and pathway databases
UniPathwayi | UPA00344 UPA00988 |
Reactomei | R-DDI-947581, Molybdenum cofactor biosynthesis |
Miscellaneous databases
PROi | PR:Q55FS0 |
Family and domain databases
Gene3Di | 3.40.250.10, 1 hit |
HAMAPi | MF_03049, MOCS3_Uba4, 1 hit |
InterProi | View protein in InterPro IPR028885, MOCS3/Uba4 IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz |
Pfami | View protein in Pfam PF00581, Rhodanese, 1 hit PF00899, ThiF, 1 hit |
SMARTi | View protein in SMART SM00450, RHOD, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBA4_DICDI | |
Accessioni | Q55FS0Primary (citable) accession number: Q55FS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2008 |
Last sequence update: | May 24, 2005 | |
Last modified: | April 7, 2021 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families