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UniProtKB - Q55D52 (MSMOA_DICDI)
Protein
Putative methylsterol monooxygenase DDB_G0270946
Gene
DDB_G0270946
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
Catalytic activityi
- 4,4-dimethyl-5α-cholest-7-en-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-4β-methyl-5α-cholest-7-ene-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2OEC:1.14.18.9
Cofactori
Fe cationBy similarity
: zymosterol biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes zymosterol from lanosterol. This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.
GO - Molecular functioni
- C-4 methylsterol oxidase activity Source: GO_Central
- iron ion binding Source: InterPro
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- sterol biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | Iron, NAD |
Enzyme and pathway databases
Reactomei | R-DDI-191273, Cholesterol biosynthesis R-DDI-192105, Synthesis of bile acids and bile salts |
UniPathwayi | UPA00770;UER00756 |
Names & Taxonomyi
Protein namesi | Recommended name: Putative methylsterol monooxygenase DDB_G0270946 (EC:1.14.18.9)Alternative name(s): C-4 methylsterol oxidase |
Gene namesi | ORF Names:DDB_G0270946 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0270946 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 31 – 51 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 72 – 92 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 110 – 130 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000380108 | 1 – 267 | Putative methylsterol monooxygenase DDB_G0270946Add BLAST | 267 |
Proteomic databases
PaxDbi | Q55D52 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 119 – 249 | Fatty acid hydroxylaseSequence analysisAdd BLAST | 131 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 132 – 136 | Histidine box-1By similarity | 5 | |
Motifi | 145 – 149 | Histidine box-2By similarity | 5 | |
Motifi | 224 – 230 | Histidine box-3By similarity | 7 |
Domaini
The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similaritiesi
Belongs to the sterol desaturase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0873, Eukaryota |
HOGENOMi | CLU_047036_5_3_1 |
InParanoidi | Q55D52 |
OMAi | MFYYAHR |
PhylomeDBi | Q55D52 |
Family and domain databases
InterProi | View protein in InterPro IPR006694, Fatty_acid_hydroxylase |
Pfami | View protein in Pfam PF04116, FA_hydroxylase, 1 hit |
i Sequence
Sequence statusi: Complete.
Q55D52-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESLSLKFIE PYWFKFVDYY GEEFLLTYGT FIAHQVFYFG CFIPFLIADF
60 70 80 90 100
IPFFRKYKIQ QTKENDWKSQ TYCAIKVILT QVLIQLPMMY IFDPAIKAIG
110 120 130 140 150
LSARAPLPSI PYLLLTLVSS FIIEDFYFYW AHRALHHGIW YKYIHKVHHD
160 170 180 190 200
YASPFGITAE YAHPLETIIL GVGTVIGPFL FSRDLFTLWV WLGVRLYQTV
210 220 230 240 250
ECHSGYDFPW SFTNLIPFWG GAPFHDYHHE VFIGNYASTF TYLDKIFGTS
260
GKSYYSRIEK KSINKSE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000005 Genomic DNA Translation: EEU04148.1 |
RefSeqi | XP_002649198.1, XM_002649152.1 |
Genome annotation databases
EnsemblProtistsi | EEU04148; EEU04148; DDB_G0270946 |
GeneIDi | 8617234 |
KEGGi | ddi:DDB_G0270946 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000005 Genomic DNA Translation: EEU04148.1 |
RefSeqi | XP_002649198.1, XM_002649152.1 |
3D structure databases
SMRi | Q55D52 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 44689.DDB0304894 |
Proteomic databases
PaxDbi | Q55D52 |
Genome annotation databases
EnsemblProtistsi | EEU04148; EEU04148; DDB_G0270946 |
GeneIDi | 8617234 |
KEGGi | ddi:DDB_G0270946 |
Organism-specific databases
dictyBasei | DDB_G0270946 |
Phylogenomic databases
eggNOGi | KOG0873, Eukaryota |
HOGENOMi | CLU_047036_5_3_1 |
InParanoidi | Q55D52 |
OMAi | MFYYAHR |
PhylomeDBi | Q55D52 |
Enzyme and pathway databases
UniPathwayi | UPA00770;UER00756 |
Reactomei | R-DDI-191273, Cholesterol biosynthesis R-DDI-192105, Synthesis of bile acids and bile salts |
Miscellaneous databases
PROi | PR:Q55D52 |
Family and domain databases
InterProi | View protein in InterPro IPR006694, Fatty_acid_hydroxylase |
Pfami | View protein in Pfam PF04116, FA_hydroxylase, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MSMOA_DICDI | |
Accessioni | Q55D52Primary (citable) accession number: Q55D52 Secondary accession number(s): C7FZW7, Q55D53 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 28, 2009 |
Last sequence update: | July 28, 2009 | |
Last modified: | February 23, 2022 | |
This is version 84 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families