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UniProtKB - Q55BH9 (DGAT1_DICDI)
Protein
Diacylglycerol O-acyltransferase 1
Gene
dgat1
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates.
By similarityCatalytic activityi
- EC:2.3.1.20By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.3.1.76By similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-glycerol = 1-O-(9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol = 1-O-(9Z-octadecenyl)-2,3-di-(9Z-octadecenoyl)glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H+ = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-3-(9Z)-octadecenoyl-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- hexadecane-1,2-diol + 2 hexadecanoyl-CoA = 1,2-O,O-dihexadecanoyl-1,2-hexadecanediol + 2 CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1,3-di-(9Z-octadecenoyl)-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 2,3-di-(9Z)-octadecenoyl-sn-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
: glycerolipid metabolism Pathwayi
This protein is involved in the pathway glycerolipid metabolism, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway glycerolipid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 532 | By similarity | 1 |
GO - Molecular functioni
- diacylglycerol O-acyltransferase activity Source: dictyBase
- O-acyltransferase activity Source: GO_Central
- retinol O-fatty-acyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- ether lipid biosynthetic process Source: dictyBase
- glycerol metabolic process Source: UniProtKB-KW
- lipid droplet formation Source: dictyBase
- monoacylglycerol biosynthetic process Source: UniProtKB
- triglyceride biosynthetic process Source: dictyBase
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Glycerol metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-DDI-1482883, Acyl chain remodeling of DAG and TAG R-DDI-6798695, Neutrophil degranulation R-DDI-75109, Triglyceride biosynthesis |
UniPathwayi | UPA00230 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dgat1 ORF Names:DDB_G0271342 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0271342, dgat1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: dictyBase
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 217 – 237 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 254 – 274 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 306 – 326 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 399 – 419 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 449 – 469 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 520 – 540 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 545 – 565 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 570 – 590 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000377488 | 1 – 617 | Diacylglycerol O-acyltransferase 1Add BLAST | 617 |
Proteomic databases
PaxDbi | Q55BH9 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 52 | DisorderedSequence analysisAdd BLAST | 52 | |
Regioni | 95 – 186 | DisorderedSequence analysisAdd BLAST | 92 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 477 – 483 | FYXDWWN motifBy similarity | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 38 | Polar residuesSequence analysisAdd BLAST | 38 | |
Compositional biasi | 95 – 146 | Polar residuesSequence analysisAdd BLAST | 52 | |
Compositional biasi | 161 – 186 | Polar residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0380, Eukaryota |
HOGENOMi | CLU_018190_0_0_1 |
InParanoidi | Q55BH9 |
OMAi | WAFMGIM |
PhylomeDBi | Q55BH9 |
Family and domain databases
InterProi | View protein in InterPro IPR027251, Diacylglycerol_acylTrfase1 IPR004299, MBOAT_fam IPR014371, Oat_ACAT_DAG_ARE |
PANTHERi | PTHR10408, PTHR10408, 1 hit PTHR10408:SF7, PTHR10408:SF7, 1 hit |
Pfami | View protein in Pfam PF03062, MBOAT, 1 hit |
PIRSFi | PIRSF000439, Oat_ACAT_DAG_ARE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q55BH9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPIPPSNGN KNNSMDKQPQ QPQQPQQQQQ QQQQQRRDQR NSKLNELNET
60 70 80 90 100
ERVRNRFISH EFHKLDRTKS RIDAPKISFS DSESESDSEF FLAKRNTNNN
110 120 130 140 150
NQNNTSPTFS SANGKQSNLT QRKINTQIQS KQPTNNNVQP LTDDEGTINH
160 170 180 190 200
SNHHHHHHNQ NNNGNNNNNN NNNNNNNKIS TPPKQEEKMT MNGLFTLRPS
210 220 230 240 250
ILSSESNGSS YRGFLNLLLI LLITASFRLV ILNHLLYGIR INLDLYKISE
260 270 280 290 300
YHRWPGVMIS LMINLFIIAA YLIEKAAAKQ LLPDRICYLL RIINCAAVII
310 320 330 340 350
VPSGSIIAFS PNPASGIIVM ILICTFSMKI ISYAYENSKQ RKLNPDNKKF
360 370 380 390 400
VIDPTNTSIY PNNLSLRSTY WFMLVPTLVY QLSYPRSPKI RKGYLLRRIV
410 420 430 440 450
EALSLSLLIL WMVNQYMLPL VQNSIEPLEK IDIVLIVERI MKLSLPNLYV
460 470 480 490 500
WLLGFYVFFH LYLNIVAEIT RFGDREFYRD WWNSTGLDYF WRTWNMPVHH
510 520 530 540 550
WMVVLIYTPM RRRGFSKNMG YFMCFFVSAI FHELVISIPF HSLKLWGFFG
560 570 580 590 600
IMSQMVLIAL TKNLMNGRNL GNVIFWISIV LGQPLVVLLY YRNFVLENPE
610
WYRNVEPPTS PPVMPFY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000006 Genomic DNA Translation: EAL71801.2 |
RefSeqi | XP_645633.2, XM_640541.2 |
Genome annotation databases
EnsemblProtistsi | EAL71801; EAL71801; DDB_G0271342 |
GeneIDi | 8617825 |
KEGGi | ddi:DDB_G0271342 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000006 Genomic DNA Translation: EAL71801.2 |
RefSeqi | XP_645633.2, XM_640541.2 |
3D structure databases
AlphaFoldDBi | Q55BH9 |
SMRi | Q55BH9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 44689.DDB0304727 |
Proteomic databases
PaxDbi | Q55BH9 |
Genome annotation databases
EnsemblProtistsi | EAL71801; EAL71801; DDB_G0271342 |
GeneIDi | 8617825 |
KEGGi | ddi:DDB_G0271342 |
Organism-specific databases
dictyBasei | DDB_G0271342, dgat1 |
Phylogenomic databases
eggNOGi | KOG0380, Eukaryota |
HOGENOMi | CLU_018190_0_0_1 |
InParanoidi | Q55BH9 |
OMAi | WAFMGIM |
PhylomeDBi | Q55BH9 |
Enzyme and pathway databases
UniPathwayi | UPA00230 |
Reactomei | R-DDI-1482883, Acyl chain remodeling of DAG and TAG R-DDI-6798695, Neutrophil degranulation R-DDI-75109, Triglyceride biosynthesis |
Miscellaneous databases
PROi | PR:Q55BH9 |
Family and domain databases
InterProi | View protein in InterPro IPR027251, Diacylglycerol_acylTrfase1 IPR004299, MBOAT_fam IPR014371, Oat_ACAT_DAG_ARE |
PANTHERi | PTHR10408, PTHR10408, 1 hit PTHR10408:SF7, PTHR10408:SF7, 1 hit |
Pfami | View protein in Pfam PF03062, MBOAT, 1 hit |
PIRSFi | PIRSF000439, Oat_ACAT_DAG_ARE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DGAT1_DICDI | |
Accessioni | Q55BH9Primary (citable) accession number: Q55BH9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 16, 2009 |
Last sequence update: | June 16, 2009 | |
Last modified: | May 25, 2022 | |
This is version 96 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families