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Protein

Cytochrome P450 119

Gene

cyp119

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The endogenous substrate is not known. In vitro, catalyzes the H2O2-dependent epoxidation of styrene, cis-beta-methylstyrene, and cis-stilbene with retention of stereochemistry. Is able to use cumene hydroperoxide (CHP) or tert-butyl hydroperoxide (TBHP) instead of H2O2 as the electron acceptor. Can also hydroxylate fatty acids such as lauric acid.3 Publications

Caution

Was originally (PubMed:8617361) thought to originate from Sulfolobus solfataricus, but was shown (PubMed:18157853) to stem from Sulfolobus acidocaldarius. This was due to a contamination of the S.solfataricus P1 strain isolate used for the initial cloning with the S.acidocaldarius species.2 Publications

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

hemeNote: Binds 1 heme group per subunit.

Kineticsi

  1. KM=21 µM for lauric acid3 Publications
  2. KM=9.2 mM for styrene3 Publications

    pH dependencei

    Optimum pH is 8.5 for peroxidase activity.3 Publications

    Temperature dependencei

    Optimum temperature is about 75 degrees Celsius. Activity is 10-fold greater at 75 degrees Celsius than that measured at 25 degrees Celsius. Thermostable up to 85 degrees Celsius. Thermal denaturation midpoint (Tm) is 91 degrees Celsius.3 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei76Heme2 Publications1
    Binding sitei80Heme2 Publications1
    Binding sitei257Heme2 Publications1
    Binding sitei259Heme2 Publications1
    Binding sitei315Heme2 Publications1
    Metal bindingi317Iron (heme axial ligand)1

    GO - Molecular functioni

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase, Peroxidase
    LigandHeme, Iron, Metal-binding

    Enzyme and pathway databases

    BRENDAi1.11.1.7 6160

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 119 (EC:1.14.-.-)
    Alternative name(s):
    Peroxidase (EC:1.11.1.7)
    Gene namesi
    Name:cyp119
    Ordered Locus Names:Saci_2081
    OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
    Taxonomic identifieri330779 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    Proteomesi
    • UP000001018 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi77D → R: 1.4-fold reduction in styrene epoxidation activity. 13-fold increase in lauric acid hydroxylation activity. 1 Publication1
    Mutagenesisi213T → A: 1.2-fold reduction in styrene epoxidation activity. No effect on thermostability. 1 Publication1
    Mutagenesisi213T → F: Loss of styrene epoxidation activity. No effect on thermostability. 1 Publication1
    Mutagenesisi213T → S: 5-fold reduction in styrene epoxidation activity. No effect on thermostability. 1 Publication1
    Mutagenesisi213T → V: 147-fold reduction in styrene epoxidation activity. No effect on thermostability. 1 Publication1
    Mutagenesisi213T → W: 19-fold reduction in styrene epoxidation activity. No effect on thermostability. 1 Publication1
    Mutagenesisi214T → A: 2.7-fold increase in styrene epoxidation activity. No effect on thermostability. 2 Publications1
    Mutagenesisi214T → V: 3-fold increase in styrene epoxidation activity. 6-fold increase in lauric acid hydroxylation activity. No effect on thermostability. 2 Publications1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000522371 – 368Cytochrome P450 119Add BLAST368

    Proteomic databases

    PRIDEiQ55080

    Interactioni

    Protein-protein interaction databases

    STRINGi330779.Saci_2081

    Structurei

    Secondary structure

    1368
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliQ55080
    SMRiQ55080
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ55080

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiarCOG02814 Archaea
    COG2124 LUCA
    HOGENOMiHOG000243678
    KOiK21201
    OMAiEEIKWQG
    OrthoDBiPOG093Z029Q

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR002397 Cyt_P450_B
    IPR017972 Cyt_P450_CS
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00359 BP450
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q55080-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MYDWFSEMRK KDPVYYDGNI WQVFSYRYTK EVLNNFSKFS SDLTGYHERL
    60 70 80 90 100
    EDLRNGKIRF DIPTRYTMLT SDPPLHDELR SMSADIFSPQ KLQTLETFIR
    110 120 130 140 150
    ETTRSLLDSI DPREDDIVKK LAVPLPIIVI SKILGLPIED KEKFKEWSDL
    160 170 180 190 200
    VAFRLGKPGE IFELGKKYLE LIGYVKDHLN SGTEVVSRVV NSNLSDIEKL
    210 220 230 240 250
    GYIILLLIAG NETTTNLISN SVIDFTRFNL WQRIREENLY LKAIEEALRY
    260 270 280 290 300
    SPPVMRTVRK TKERVKLGDQ TIEEGEYVRV WIASANRDEE VFHDGEKFIP
    310 320 330 340 350
    DRNPNPHLSF GSGIHLCLGA PLARLEARIA IEEFSKRFRH IEILDTEKVP
    360
    NEVLNGYKRL VVRLKSNE
    Length:368
    Mass (Da):42,863
    Last modified:November 1, 1996 - v1
    Checksum:iE2A5C4F064537E8C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U51337 Genomic DNA Translation: AAB03278.1
    CP000077 Genomic DNA Translation: AAY81375.1
    PIRiS71328
    RefSeqiWP_011278877.1, NC_007181.1

    Genome annotation databases

    EnsemblBacteriaiAAY81375; AAY81375; Saci_2081
    GeneIDi3472594
    KEGGisai:Saci_2081
    PATRICifig|330779.12.peg.2081

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U51337 Genomic DNA Translation: AAB03278.1
    CP000077 Genomic DNA Translation: AAY81375.1
    PIRiS71328
    RefSeqiWP_011278877.1, NC_007181.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1F4TX-ray1.93A/B1-368[»]
    1F4UX-ray2.69A/B1-368[»]
    1IO7X-ray1.50A/B1-368[»]
    1IO8X-ray2.00A/B1-368[»]
    1IO9X-ray2.05A/B1-368[»]
    4TT5X-ray2.18A1-368[»]
    4TUVX-ray2.50A1-368[»]
    4WPDX-ray2.00A/B1-368[»]
    4WQJX-ray2.70A1-368[»]
    5BV5X-ray2.70A/B/C/D1-368[»]
    ProteinModelPortaliQ55080
    SMRiQ55080
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi330779.Saci_2081

    Proteomic databases

    PRIDEiQ55080

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAY81375; AAY81375; Saci_2081
    GeneIDi3472594
    KEGGisai:Saci_2081
    PATRICifig|330779.12.peg.2081

    Phylogenomic databases

    eggNOGiarCOG02814 Archaea
    COG2124 LUCA
    HOGENOMiHOG000243678
    KOiK21201
    OMAiEEIKWQG
    OrthoDBiPOG093Z029Q

    Enzyme and pathway databases

    BRENDAi1.11.1.7 6160

    Miscellaneous databases

    EvolutionaryTraceiQ55080

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR002397 Cyt_P450_B
    IPR017972 Cyt_P450_CS
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00359 BP450
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCP119_SULAC
    AccessioniPrimary (citable) accession number: Q55080
    Secondary accession number(s): Q4J756
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 25, 2017
    This is version 117 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
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