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Protein

Glutamate decarboxylase A

Gene

gadA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Generates GABA from glutamate. GABA induces the release of acbA from prespore cells and induces the exposure of tagC on the surface of prestalk cells where it can convert acbA to SDF-2. Glutamate acts as a competitive inhibitor.1 Publication

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62SubstrateBy similarity1
Binding sitei83SubstrateBy similarity1
Binding sitei210Pyridoxal phosphateBy similarity1
Binding sitei273Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandPyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase A (EC:4.1.1.15)
Gene namesi
Name:gadA
ORF Names:DDB_G0280199
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0280199 gadA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: dictyBase

Pathology & Biotechi

Disruption phenotypei

Mutant cells grow and develop well, forming normally proportioned fruiting bodies, but the number of viable spores is reduced to about half.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003913291 – 462Glutamate decarboxylase AAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei274N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ54VQ5

Expressioni

Tissue specificityi

Exclusively expressed in prespore cells late in development.1 Publication

Developmental stagei

Expressed only after 10 hours of development and its mRNA accumulates to peak at 18 hours when cells are initiating fruiting body formation. Highly expressed during phagocytosis of non-pathogenic bacteria.2 Publications

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0231446

Structurei

3D structure databases

ProteinModelPortaliQ54VQ5
SMRiQ54VQ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125Pyridoxal phosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiKOG1383 Eukaryota
COG0076 LUCA
InParanoidiQ54VQ5
KOiK01580
OMAiRPNLVMG
PhylomeDBiQ54VQ5

Family and domain databases

Gene3Di3.40.640.10, 1 hit
InterProiView protein in InterPro
IPR010107 Glutamate_decarboxylase
IPR002129 PyrdxlP-dep_de-COase
IPR015424 PyrdxlP-dep_Trfase
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR43321 PTHR43321, 1 hit
PfamiView protein in Pfam
PF00282 Pyridoxal_deC, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01788 Glu-decarb-GAD, 1 hit

Sequencei

Sequence statusi: Complete.

Q54VQ5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLHHVKTNK YKGFYDSYAS PPATKEISKY SLKEESNKPE VIRDLIIDEL
60 70 80 90 100
LLDGNAKQNL ATFCQTDLDK EIHVLMDKCI DKNMIDKDEY PQTAEIESRC
110 120 130 140 150
VHILADLWNS PESSETIGCS TTGSSEAAML GGMALKWRWR ENRKKQGKPF
160 170 180 190 200
DKPNIVTGPV QICWHKFALY FDIELREVPM EHDRYIMTPE EAIKRCDENT
210 220 230 240 250
IGVIPTLGVT FTLQYEDVKG ISEALDKFEK ETGLDIPIHV DAASGGFVAP
260 270 280 290 300
FLQPEIVWDF RLPRVKSING SGHKFGLSPL GVGWVVWRGK NDIHKDLVFD
310 320 330 340 350
VNYLGGNMPT FSLNFSRPGG QIVCQYYNFL RHGRSGYTAV HQACLDHGIF
360 370 380 390 400
ISKQVKKHGI FDIVYDGTGA LPGACWKLKK DAKVKFNLFD LSDRMRNRGW
410 420 430 440 450
QIASYTLPNL PDIVVQRVLI RHGFSHDMAM LLIEDFNRSI EYFDKHPMVQ
460
SISKDEGQSF HH
Length:462
Mass (Da):52,621
Last modified:May 24, 2005 - v1
Checksum:i5DFE4AB49DD215CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000035 Genomic DNA Translation: EAL67326.1
RefSeqiXP_641300.1, XM_636208.1

Genome annotation databases

EnsemblProtistsiEAL67326; EAL67326; DDB_G0280199
GeneIDi8622433
KEGGiddi:DDB_G0280199

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000035 Genomic DNA Translation: EAL67326.1
RefSeqiXP_641300.1, XM_636208.1

3D structure databases

ProteinModelPortaliQ54VQ5
SMRiQ54VQ5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231446

Proteomic databases

PaxDbiQ54VQ5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL67326; EAL67326; DDB_G0280199
GeneIDi8622433
KEGGiddi:DDB_G0280199

Organism-specific databases

dictyBaseiDDB_G0280199 gadA

Phylogenomic databases

eggNOGiKOG1383 Eukaryota
COG0076 LUCA
InParanoidiQ54VQ5
KOiK01580
OMAiRPNLVMG
PhylomeDBiQ54VQ5

Miscellaneous databases

PROiPR:Q54VQ5

Family and domain databases

Gene3Di3.40.640.10, 1 hit
InterProiView protein in InterPro
IPR010107 Glutamate_decarboxylase
IPR002129 PyrdxlP-dep_de-COase
IPR015424 PyrdxlP-dep_Trfase
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR43321 PTHR43321, 1 hit
PfamiView protein in Pfam
PF00282 Pyridoxal_deC, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01788 Glu-decarb-GAD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGADA_DICDI
AccessioniPrimary (citable) accession number: Q54VQ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: May 24, 2005
Last modified: February 28, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
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Main funding by: National Institutes of Health

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