UniProtKB - Q54VA2 (FUMH_DICDI)
Fumarate hydratase, mitochondrial
fumH
Functioni
Catalyzes the reversible stereospecific interconversion of fumarate to L-malate.
By similarityCatalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH.
By similarityMiscellaneous
Catalytic activityi
- EC:4.2.1.2By similarity
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.By similarity This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 208 | SubstrateBy similarity | 1 | |
Active sitei | 209 | Proton donor/acceptorBy similarity | 1 | |
Active sitei | 339 | By similarity | 1 | |
Binding sitei | 340 | SubstrateBy similarity | 1 | |
Sitei | 352 | Important for catalytic activityBy similarity | 1 |
GO - Molecular functioni
- fumarate hydratase activity Source: GO_Central
GO - Biological processi
- fumarate metabolic process Source: GO_Central
- malate metabolic process Source: GO_Central
- response to bacterium Source: dictyBase
- tricarboxylic acid cycle Source: GO_Central
Keywordsi
Molecular function | Lyase |
Biological process | Tricarboxylic acid cycle |
Enzyme and pathway databases
Reactomei | R-DDI-71403, Citric acid cycle (TCA cycle) |
UniPathwayi | UPA00223;UER01007 |
Names & Taxonomyi
Protein namesi | Recommended name: Fumarate hydratase, mitochondrialBy similarity (EC:4.2.1.2By similarity)Short name: FumaraseBy similarity |
Gene namesi | Name:fumHImported ORF Names:DDB_G0280495 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0280495, fumH |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Mitochondrion
- mitochondrion Source: dictyBase
Other locations
- cytoplasm Source: dictyBase
- phagocytic vesicle Source: dictyBase
- tricarboxylic acid cycle enzyme complex Source: InterPro
Keywords - Cellular componenti
Cytoplasm, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 19 | MitochondrionSequence analysisAdd BLAST | 19 | |
ChainiPRO_0000331360 | 20 – 485 | Fumarate hydratase, mitochondrialAdd BLAST | 466 |
Proteomic databases
PaxDbi | Q54VA2 |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 44689.DDB0231397 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 118 – 120 | Substrate bindingBy similarity | 3 | |
Regioni | 150 – 153 | Substrate binding (B site)By similarity | 4 | |
Regioni | 160 – 162 | Substrate bindingBy similarity | 3 | |
Regioni | 345 – 347 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1317, Eukaryota |
InParanoidi | Q54VA2 |
OMAi | HDSMGEV |
PhylomeDBi | Q54VA2 |
Family and domain databases
CDDi | cd01362, Fumarase_classII, 1 hit |
Gene3Di | 1.10.275.10, 1 hit |
HAMAPi | MF_00743, FumaraseC, 1 hit |
InterProi | View protein in InterPro IPR005677, Fum_hydII IPR024083, Fumarase/histidase_N IPR018951, Fumarase_C_C IPR020557, Fumarate_lyase_CS IPR000362, Fumarate_lyase_fam IPR022761, Fumarate_lyase_N IPR008948, L-Aspartase-like |
PANTHERi | PTHR11444, PTHR11444, 1 hit |
Pfami | View protein in Pfam PF10415, FumaraseC_C, 1 hit PF00206, Lyase_1, 1 hit |
PRINTSi | PR00149, FUMRATELYASE |
SUPFAMi | SSF48557, SSF48557, 1 hit |
TIGRFAMsi | TIGR00979, fumC_II, 1 hit |
PROSITEi | View protein in PROSITE PS00163, FUMARATE_LYASES, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLSASRKLNN QQFLKTIRNM TTFRSEFDTF GEVKVNDEKY WGAQTQRSLE
60 70 80 90 100
NFDIGGESEK MPLMVVRSFG ILKRCAAIVN KKYGLDATIA DNIAKAATEV
110 120 130 140 150
VEGKLDDQFP LVVFQTGSGT QSNMNANEVI SNRAIELMTG KRDFSKKLVH
160 170 180 190 200
PNDHVNKSQS SNDTFPTCMH IAAAISINEK LVPALEMLLA AMRTKQNEFN
210 220 230 240 250
HIIKIGRTHL QDATPLTLGQ EFSGYCTQIE YGIQRIKDTL PRLYNLAQGG
260 270 280 290 300
TAVGTGLNTP VGFDVDIASE VAKFTGLPFK TAPNKFEALA AHDAMVEVSG
310 320 330 340 350
ALNTVAVSLM KIANDIRFLG SGPRCGLGEL ILPENEPGSS IMPGKVNPTQ
360 370 380 390 400
CEAMTMVCAQ VMGNNTTVSI AGSNGHFELN VFKPVIIKNV LSSIRLIADA
410 420 430 440 450
SVSFTKHCVV GIKADEKRID QLLHESLMLV TALNPYIGYD KAAKAAKKAH
460 470 480
KEKTTLKEAC LSLGFTTSEE FDKWVDPSKM IGSMK
The sequence of this isoform differs from the canonical sequence as follows:
1-19: Missing.
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_033185 | 1 – 19 | Missing in isoform Cytoplasmic. CuratedAdd BLAST | 19 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000036 Genomic DNA Translation: EAL67226.1 AAFI02000036 Genomic DNA Translation: EAL67227.1 |
RefSeqi | XP_001134561.1, XM_001134561.1 XP_641207.1, XM_636115.1 |
Genome annotation databases
EnsemblProtistsi | EAL67226; EAL67226; DDB_G0280495 EAL67227; EAL67227; DDB_G0280495 |
GeneIDi | 8622588 |
KEGGi | ddi:DDB_G0280495 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000036 Genomic DNA Translation: EAL67226.1 AAFI02000036 Genomic DNA Translation: EAL67227.1 |
RefSeqi | XP_001134561.1, XM_001134561.1 XP_641207.1, XM_636115.1 |
3D structure databases
SMRi | Q54VA2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 44689.DDB0231397 |
Proteomic databases
PaxDbi | Q54VA2 |
Genome annotation databases
EnsemblProtistsi | EAL67226; EAL67226; DDB_G0280495 EAL67227; EAL67227; DDB_G0280495 |
GeneIDi | 8622588 |
KEGGi | ddi:DDB_G0280495 |
Organism-specific databases
dictyBasei | DDB_G0280495, fumH |
Phylogenomic databases
eggNOGi | KOG1317, Eukaryota |
InParanoidi | Q54VA2 |
OMAi | HDSMGEV |
PhylomeDBi | Q54VA2 |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER01007 |
Reactomei | R-DDI-71403, Citric acid cycle (TCA cycle) |
Miscellaneous databases
PROi | PR:Q54VA2 |
Family and domain databases
CDDi | cd01362, Fumarase_classII, 1 hit |
Gene3Di | 1.10.275.10, 1 hit |
HAMAPi | MF_00743, FumaraseC, 1 hit |
InterProi | View protein in InterPro IPR005677, Fum_hydII IPR024083, Fumarase/histidase_N IPR018951, Fumarase_C_C IPR020557, Fumarate_lyase_CS IPR000362, Fumarate_lyase_fam IPR022761, Fumarate_lyase_N IPR008948, L-Aspartase-like |
PANTHERi | PTHR11444, PTHR11444, 1 hit |
Pfami | View protein in Pfam PF10415, FumaraseC_C, 1 hit PF00206, Lyase_1, 1 hit |
PRINTSi | PR00149, FUMRATELYASE |
SUPFAMi | SSF48557, SSF48557, 1 hit |
TIGRFAMsi | TIGR00979, fumC_II, 1 hit |
PROSITEi | View protein in PROSITE PS00163, FUMARATE_LYASES, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FUMH_DICDI | |
Accessioni | Q54VA2Primary (citable) accession number: Q54VA2 Secondary accession number(s): Q54VA1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2008 |
Last sequence update: | May 24, 2005 | |
Last modified: | February 23, 2022 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families