UniProtKB - Q54T81 (DKC1_DICDI)
Protein
Probable H/ACA ribonucleoprotein complex subunit 4
Gene
nola4
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs (By similarity).By similarity
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 109 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- pseudouridine synthase activity Source: GO_Central
- RNA binding Source: dictyBase
- telomerase activity Source: dictyBase
GO - Biological processi
- box H/ACA RNA 3'-end processing Source: GO_Central
- cellular response to DNA damage stimulus Source: dictyBase
- mRNA pseudouridine synthesis Source: GO_Central
- response to mercury ion Source: dictyBase
- response to oxidative stress Source: dictyBase
- rRNA pseudouridine synthesis Source: GO_Central
- snRNA pseudouridine synthesis Source: GO_Central
Keywordsi
Molecular function | Isomerase, Ribonucleoprotein, RNA-binding |
Biological process | Ribosome biogenesis, rRNA processing |
Enzyme and pathway databases
Reactomei | R-DDI-171319, Telomere Extension By Telomerase |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:nola4 Synonyms:dkc1 ORF Names:DDB_G0281933 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0281933, nola4 |
Subcellular locationi
Nucleus
- nucleolus By similarity
Nucleus
- box H/ACA snoRNP complex Source: GO_Central
- nucleolus Source: dictyBase
- telomerase holoenzyme complex Source: dictyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000331202 | 1 – 540 | Probable H/ACA ribonucleoprotein complex subunit 4Add BLAST | 540 |
Proteomic databases
PaxDbi | Q54T81 |
Interactioni
Subunit structurei
Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs).
By similarityProtein-protein interaction databases
IntActi | Q54T81, 1 interactor |
STRINGi | 44689.DDB0233679 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 280 – 355 | PUAPROSITE-ProRule annotationAdd BLAST | 76 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 448 – 494 | Sequence analysisAdd BLAST | 47 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 409 – 413 | Poly-Thr | 5 |
Sequence similaritiesi
Belongs to the pseudouridine synthase TruB family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2529, Eukaryota |
HOGENOMi | CLU_032087_3_2_1 |
InParanoidi | Q54T81 |
OMAi | PRRWGMG |
PhylomeDBi | Q54T81 |
Family and domain databases
InterProi | View protein in InterPro IPR012960, Dyskerin-like IPR020103, PsdUridine_synth_cat_dom_sf IPR002501, PsdUridine_synth_N IPR002478, PUA IPR015947, PUA-like_sf IPR004802, tRNA_PsdUridine_synth_B_fam IPR032819, TruB_C IPR004521, Uncharacterised_CHP00451 |
PANTHERi | PTHR23127, PTHR23127, 1 hit |
Pfami | View protein in Pfam PF08068, DKCLD, 1 hit PF01472, PUA, 1 hit PF16198, TruB_C_2, 1 hit PF01509, TruB_N, 1 hit |
SMARTi | View protein in SMART SM01136, DKCLD, 1 hit SM00359, PUA, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00425, CBF5, 1 hit TIGR00451, unchar_dom_2, 1 hit |
PROSITEi | View protein in PROSITE PS50890, PUA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q54T81-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTDKKSKSK SSEKSTQEVE QVIKPEKTPI LDTSKWPLLL KNYDQLSVRT
60 70 80 90 100
GHYTPIPNGH SPLKRPIKEY VKYGIINLDK PSNPSSHEVV AWIRTILRVT
110 120 130 140 150
KTGHSGTLDP KVTGCLIVCI ERATRLVKSQ QGAGKEYIGI VRLHGAIEGT
160 170 180 190 200
TELSKAVDTL TGALFQRPPQ KSAVKKRLRV RTIHNSKLLE FDPERNLGLI
210 220 230 240 250
WVDCEAGTYI RTLCVHIGLL MGVGGHMQEL RRVRSGIMSE KKGQVTMHDV
260 270 280 290 300
KDAQYEYDNS KDESYLRRVI QPLEAILTNY KRIVVKDSAI NAICYGAKLM
310 320 330 340 350
IPGLLRYEQD IESNEEVVLI TTKGEAIAIG IAQMTTASMA TCDHGVVATI
360 370 380 390 400
KRVIMDRDVY PVRWGLGPKA KVKKEMIKDG KLDKFGKPNE KTPSDWTNTY
410 420 430 440 450
TYYTGQVATT TTTSPVESMN VDTPKKATAS VAVKAEVESS EDEKPVPKKE
460 470 480 490 500
KKDKKEKKKD SSDDESEEEK SSKKDKKEKK EKKEKKEKKS SKDDSDDESS
510 520 530 540
KKEKKDKKRK KSSDKDSDSE KESSDKKDKK DKKEKKKSKN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000043 Genomic DNA Translation: EAL66526.1 |
RefSeqi | XP_640508.1, XM_635416.1 |
Genome annotation databases
EnsemblProtistsi | EAL66526; EAL66526; DDB_G0281933 |
GeneIDi | 8623323 |
KEGGi | ddi:DDB_G0281933 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000043 Genomic DNA Translation: EAL66526.1 |
RefSeqi | XP_640508.1, XM_635416.1 |
3D structure databases
SMRi | Q54T81 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q54T81, 1 interactor |
STRINGi | 44689.DDB0233679 |
Proteomic databases
PaxDbi | Q54T81 |
Genome annotation databases
EnsemblProtistsi | EAL66526; EAL66526; DDB_G0281933 |
GeneIDi | 8623323 |
KEGGi | ddi:DDB_G0281933 |
Organism-specific databases
dictyBasei | DDB_G0281933, nola4 |
Phylogenomic databases
eggNOGi | KOG2529, Eukaryota |
HOGENOMi | CLU_032087_3_2_1 |
InParanoidi | Q54T81 |
OMAi | PRRWGMG |
PhylomeDBi | Q54T81 |
Enzyme and pathway databases
Reactomei | R-DDI-171319, Telomere Extension By Telomerase |
Miscellaneous databases
PROi | PR:Q54T81 |
Family and domain databases
InterProi | View protein in InterPro IPR012960, Dyskerin-like IPR020103, PsdUridine_synth_cat_dom_sf IPR002501, PsdUridine_synth_N IPR002478, PUA IPR015947, PUA-like_sf IPR004802, tRNA_PsdUridine_synth_B_fam IPR032819, TruB_C IPR004521, Uncharacterised_CHP00451 |
PANTHERi | PTHR23127, PTHR23127, 1 hit |
Pfami | View protein in Pfam PF08068, DKCLD, 1 hit PF01472, PUA, 1 hit PF16198, TruB_C_2, 1 hit PF01509, TruB_N, 1 hit |
SMARTi | View protein in SMART SM01136, DKCLD, 1 hit SM00359, PUA, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00425, CBF5, 1 hit TIGR00451, unchar_dom_2, 1 hit |
PROSITEi | View protein in PROSITE PS50890, PUA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DKC1_DICDI | |
Accessioni | Q54T81Primary (citable) accession number: Q54T81 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2008 |
Last sequence update: | May 24, 2005 | |
Last modified: | April 7, 2021 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - SIMILARITY comments
Index of protein domains and families