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Protein

Calcium-binding mitochondrial carrier protein

Gene

mcfO

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane.By similarity

Miscellaneous

Binds to one calcium ion with high affinity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi145CalciumBy similarity1
Metal bindingi156CalciumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi145 – 1561PROSITE-ProRule annotationBy similarityAdd BLAST12
Calcium bindingi179 – 1902PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi360 – 3713PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DDI-70263 Gluconeogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding mitochondrial carrier protein
Alternative name(s):
Mitochondrial substrate carrier family protein O
Gene namesi
Name:mcfO
ORF Names:DDB_G0283329
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283329 mcfO

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei442 – 459Helical; Name=1By similarityAdd BLAST18
Transmembranei501 – 520Helical; Name=2By similarityAdd BLAST20
Transmembranei545 – 558Helical; Name=3By similarityAdd BLAST14
Transmembranei591 – 610Helical; Name=4By similarityAdd BLAST20
Transmembranei630 – 647Helical; Name=5By similarityAdd BLAST18
Transmembranei687 – 706Helical; Name=6By similarityAdd BLAST20

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003294281 – 772Calcium-binding mitochondrial carrier proteinAdd BLAST772

Proteomic databases

PaxDbiQ54RB9
PRIDEiQ54RB9

Interactioni

Subunit structurei

Homodimer (via N-terminus).By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ54RB9
SMRiQ54RB9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini132 – 165EF-hand 1PROSITE-ProRule annotationAdd BLAST34
Domaini166 – 201EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini235 – 270EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini347 – 382EF-hand 4PROSITE-ProRule annotationAdd BLAST36
Repeati436 – 526Solcar 1PROSITE-ProRule annotationAdd BLAST91
Repeati535 – 616Solcar 2PROSITE-ProRule annotationAdd BLAST82
Repeati624 – 712Solcar 3PROSITE-ProRule annotationAdd BLAST89

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 377N-terminal domainBy similarityAdd BLAST377
Regioni378 – 422Linker loop domainBy similarityAdd BLAST45
Regioni432 – 720Carrier domainBy similarityAdd BLAST289
Regioni721 – 772C-terminal domainBy similarityAdd BLAST52

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi36 – 42Poly-Asn7

Domaini

Upon calcium binding, the EF-hand-containing regulatory N-terminal domain binds to the C-terminal domain, opening a vestibule which allows the substrates to be translocated through the carrier domain. In the absence of calcium, the linker loop domain may close the vestibule, which may prevent substrates from entering the carrier domain.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0751 Eukaryota
ENOG410XNRM LUCA
InParanoidiQ54RB9
KOiK15105
OMAiMQNQRGT
PhylomeDBiQ54RB9

Family and domain databases

CDDicd00051 EFh, 1 hit
Gene3Di1.50.40.10, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR002067 Mit_carrier
IPR018108 Mitochondrial_sb/sol_carrier
IPR023395 Mt_carrier_dom_sf
PfamiView protein in Pfam
PF00036 EF-hand_1, 1 hit
PF13833 EF-hand_8, 2 hits
PF00153 Mito_carr, 3 hits
PRINTSiPR00926 MITOCARRIER
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SUPFAMiSSF103506 SSF103506, 1 hit
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 4 hits
PS50920 SOLCAR, 3 hits

Sequencei

Sequence statusi: Complete.

Q54RB9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFANRVRQAQ KLYQKRFFSL GNNSTISKQQ FKNSSNNNNN KNGGNKNGFY
60 70 80 90 100
QKAFIATTVA LTTTLLTATT LLDDNTNSEK EILKSQRQTF EKYASTTLEG
110 120 130 140 150
ERQMTAEDFL SALTTLESDK QSGEHEILKA SLDADKFKVL FQMADVDHTG
160 170 180 190 200
YISFDEYVMF DELMAKPEAE YFLAFKLFDR DGNGYISKND FKHVITASLD
210 220 230 240 250
PSIPFNFDCE LVNLYFGDGR TELNYSQFTQ LLKDLQQERI KQEFKFHDKY
260 270 280 290 300
NSGYIPRDKF AKVLGSVKLR KIPDHVRDKL ESISELNLLS GHPNEVSYSQ
310 320 330 340 350
FVAANDMLLH IPSYGRVLKA AILKNKKDNI NKEEFLTEAR SSTSIEITPL
360 370 380 390 400
EIDLIFHLFD LNKDGKLSIS DFEKSTGLNI NKIGGGTNYS DSYPSDSHVT
410 420 430 440 450
IQNSSTTPSP STPITNTAAA IALNKKHGKT FAQQVLESIE NFALGSIAGG
460 470 480 490 500
IGAAAVYPID LVKTRMQNQR AVDPAKRLYV NSWDCFKKVV KFEGVRGLYK
510 520 530 540 550
GILPQMVGVA PEKAIKLTVN DLLRDLFGDK SKGEIYFPLE VLAGGFAGMS
560 570 580 590 600
QVCVTNPLEI VKIRLQVQST GPKVSAITII KELGLAGLYK GAGACLLRDI
610 620 630 640 650
PFSAIYFPTY AKMKTILANE DGKLGPMDLL LAGAVAGIPA ASLVTPADVI
660 670 680 690 700
KTRLQVKANA GEQTYTGIRD CFQKILKEEG PRALFKGALA RVFRSSPQFG
710 720 730 740 750
VTLVSYELLQ KALLPDAEYK PPTNAPITQK DFDVIRGNTN TVQRVIDMES
760 770
KFGTLHQTRD NNKSSNGGEN KN
Length:772
Mass (Da):85,648
Last modified:May 24, 2005 - v1
Checksum:iC7273988F0EF53FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000052 Genomic DNA Translation: EAL65805.1
RefSeqiXP_639128.1, XM_634036.1

Genome annotation databases

EnsemblProtistsiEAL65805; EAL65805; DDB_G0283329
GeneIDi8623999
KEGGiddi:DDB_G0283329

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000052 Genomic DNA Translation: EAL65805.1
RefSeqiXP_639128.1, XM_634036.1

3D structure databases

ProteinModelPortaliQ54RB9
SMRiQ54RB9
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ54RB9
PRIDEiQ54RB9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL65805; EAL65805; DDB_G0283329
GeneIDi8623999
KEGGiddi:DDB_G0283329

Organism-specific databases

dictyBaseiDDB_G0283329 mcfO

Phylogenomic databases

eggNOGiKOG0751 Eukaryota
ENOG410XNRM LUCA
InParanoidiQ54RB9
KOiK15105
OMAiMQNQRGT
PhylomeDBiQ54RB9

Enzyme and pathway databases

ReactomeiR-DDI-70263 Gluconeogenesis

Miscellaneous databases

PROiPR:Q54RB9

Family and domain databases

CDDicd00051 EFh, 1 hit
Gene3Di1.50.40.10, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR002067 Mit_carrier
IPR018108 Mitochondrial_sb/sol_carrier
IPR023395 Mt_carrier_dom_sf
PfamiView protein in Pfam
PF00036 EF-hand_1, 1 hit
PF13833 EF-hand_8, 2 hits
PF00153 Mito_carr, 3 hits
PRINTSiPR00926 MITOCARRIER
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SUPFAMiSSF103506 SSF103506, 1 hit
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 4 hits
PS50920 SOLCAR, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCMC_DICDI
AccessioniPrimary (citable) accession number: Q54RB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 20, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
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Main funding by: National Institutes of Health

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