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UniProtKB - Q54QG6 (ALG6_DICDI)
Protein
Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
Gene
alg6
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc2-PP-Dol (By similarity).
By similarityCatalytic activityi
- α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl β-D-glucosyl phosphate = α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl phosphate + H+EC:2.4.1.267
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: GO_Central
GO - Biological processi
- dolichol-linked oligosaccharide biosynthetic process Source: InterPro
- oligosaccharide-lipid intermediate biosynthetic process Source: GO_Central
- protein N-linked glycosylation Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-DDI-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
UniPathwayi | UPA00378 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.267)Alternative name(s): Asparagine-linked glycosylation protein 6 homolog Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase |
Gene namesi | Name:alg6 ORF Names:DDB_G0283841 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0283841, alg6 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Multi-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 8 – 28 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 127 – 147 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 151 – 171 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 174 – 194 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 215 – 235 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 247 – 267 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 290 – 310 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 311 – 331 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 339 – 359 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 368 – 388 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 396 – 416 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 451 – 471 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 484 – 504 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000327819 | 1 – 518 | Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST | 518 |
Proteomic databases
PaxDbi | Q54QG6 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ALG6/ALG8 glucosyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2575, Eukaryota |
HOGENOMi | CLU_008110_3_0_1 |
InParanoidi | Q54QG6 |
OMAi | RQWYFNT |
PhylomeDBi | Q54QG6 |
Family and domain databases
InterProi | View protein in InterPro IPR039488, ALG6 IPR004856, Glyco_trans_ALG6/ALG8 |
PANTHERi | PTHR12413, PTHR12413, 1 hit PTHR12413:SF1, PTHR12413:SF1, 1 hit |
Pfami | View protein in Pfam PF03155, Alg6_Alg8, 1 hit |
i Sequence
Sequence statusi: Complete.
Q54QG6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSLNNKVGF SSLNISIVLL ILMISLLARY LVSLNGYSGQ SKPPMFGDYE
60 70 80 90 100
AQRHWMEITT NLDIHQWYFN STDNDLMYWG LDYPPLTAYL SWVFGKIGEF
110 120 130 140 150
IEPKSMELFT SRGYETDSGK LFMRMTVIVS DLFIWLPSVW FFVKTFYKQR
160 170 180 190 200
NISQQISAFL FISLQPGLLL IDHGHFQYNG VSLGLGLFAI TFIIRDQQLL
210 220 230 240 250
ASFFFVLSLN YKQMCLYYSP AFFFYLLLSN FEFTLKFSKI FSSIFKILKI
260 270 280 290 300
GIVVIFTFIL CWIPFLSIEQ ASQVLFRLFP FARGLYEDKV ANFWCFISII
310 320 330 340 350
INVKNLFTTD QLIKICLILT LVTMLPLVYG IKRIPKNKFV FIHSLINSSF
360 370 380 390 400
SFFLFSFQVH EKTILLVSLP ISLLILHHPN MVWWFILIST FSMFPLLFKD
410 420 430 440 450
GLVIPYFAIM ILYIVIGYQF KNSITRSNNQ FKHQNSQENL LASSDKSFYS
460 470 480 490 500
IYLNYWFYLN IIGMVVCHLL YQFAPHPPHL PSLWLLLVCN FSFIHFILTF
510
FYFIKEMTTF SPIKIKSN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000057 Genomic DNA Translation: EAL65535.1 |
RefSeqi | XP_638905.1, XM_633813.1 |
Genome annotation databases
EnsemblProtistsi | EAL65535; EAL65535; DDB_G0283841 |
GeneIDi | 8624302 |
KEGGi | ddi:DDB_G0283841 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000057 Genomic DNA Translation: EAL65535.1 |
RefSeqi | XP_638905.1, XM_633813.1 |
3D structure databases
AlphaFoldDBi | Q54QG6 |
SMRi | Q54QG6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 44689.DDB0231451 |
Proteomic databases
PaxDbi | Q54QG6 |
Genome annotation databases
EnsemblProtistsi | EAL65535; EAL65535; DDB_G0283841 |
GeneIDi | 8624302 |
KEGGi | ddi:DDB_G0283841 |
Organism-specific databases
dictyBasei | DDB_G0283841, alg6 |
Phylogenomic databases
eggNOGi | KOG2575, Eukaryota |
HOGENOMi | CLU_008110_3_0_1 |
InParanoidi | Q54QG6 |
OMAi | RQWYFNT |
PhylomeDBi | Q54QG6 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-DDI-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
Miscellaneous databases
PROi | PR:Q54QG6 |
Family and domain databases
InterProi | View protein in InterPro IPR039488, ALG6 IPR004856, Glyco_trans_ALG6/ALG8 |
PANTHERi | PTHR12413, PTHR12413, 1 hit PTHR12413:SF1, PTHR12413:SF1, 1 hit |
Pfami | View protein in Pfam PF03155, Alg6_Alg8, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALG6_DICDI | |
Accessioni | Q54QG6Primary (citable) accession number: Q54QG6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | May 24, 2005 | |
Last modified: | May 25, 2022 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families