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Entry version 95 (07 Oct 2020)
Sequence version 1 (24 May 2005)
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Protein

Ras guanine nucleotide exchange factor A

Gene

gefA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Ras-bound GDP/GTP exchange factor required for normal activation of adenylyl cyclase (PubMed:17380187). Component of the Sca1 complex, a regulator of cell motility, chemotaxis and signal relay (PubMed:8793298, PubMed:16234315, PubMed:20493808). The Sca1 complex is recruited to the plasma membrane in a chemoattractant- and F-actin-dependent manner and is enriched at the leading edge of chemotaxing cells where it regulates F-actin dynamics and signal relay by controlling the activation of rasC and the downstream target of rapamycin complex 2 (TORC2)-Akt/protein kinase B (PKB) pathway (PubMed:20493808).4 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-DDI-1169092, Activation of RAS in B cells
R-DDI-354192, Integrin signaling
R-DDI-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-DDI-392517, Rap1 signalling
R-DDI-5673001, RAF/MAP kinase cascade

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ras guanine nucleotide exchange factor A
Short name:
Aimless
Alternative name(s):
RasGEF domain-containing protein A
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:gefA
Synonyms:aleA, rasGEFA
ORF Names:DDB_G0284329
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDictyostelium discoideum (Slime mold)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri44689 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaAmoebozoaEvoseaEumycetozoaDictyosteliaDictyostelialesDictyosteliaceaeDictyostelium
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002195 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

Dictyostelium discoideum online informatics resource

More...
dictyBasei
DDB_G0284329, gefA

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Grows at a normal rate, but are severely impaired in both chemotaxis and activation of adenylyl cyclase, both of which are critical for the early stages of development (PubMed:8793298, PubMed:10473630, PubMed:20493808). Unable to aggregate (PubMed:8793298).3 Publications

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003844591 – 605Ras guanine nucleotide exchange factor AAdd BLAST605

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q54PQ4

PRoteomics IDEntifications database

More...
PRIDEi
Q54PQ4

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expressed during development; especially until 8 hours of development (PubMed:16086850). Expression is higher in the tip regions of slugs and early culminants (PubMed:11422293).2 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the Sca1 complex composed of at least gefA, gefH, scaA, phr, and the protein phosphatase 2A subunits pppA and pho2B (PubMed:20493808).

Interacts directly with gefH (PubMed:20493808).

1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
44689.DDB0191187

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q54PQ4

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini67 – 99LisHPROSITE-ProRule annotationAdd BLAST33
Domaini198 – 320N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST123
Domaini353 – 597Ras-GEFPROSITE-ProRule annotationAdd BLAST245

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3417, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_452309_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q54PQ4

Identification of Orthologs from Complete Genome Data

More...
OMAi
ITAICEP

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q54PQ4

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00155, RasGEF, 1 hit
cd06224, REM, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.840.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006594, LisH
IPR008937, Ras-like_GEF
IPR000651, Ras-like_Gua-exchang_fac_N
IPR023578, Ras_GEF_dom_sf
IPR001895, RASGEF_cat_dom
IPR036964, RASGEF_cat_dom_sf

The PANTHER Classification System

More...
PANTHERi
PTHR23113, PTHR23113, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00617, RasGEF, 1 hit
PF00618, RasGEF_N, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00667, LisH, 1 hit
SM00147, RasGEF, 1 hit
SM00229, RasGEFN, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48366, SSF48366, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50896, LISH, 1 hit
PS50009, RASGEF_CAT, 1 hit
PS50212, RASGEF_NTER, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q54PQ4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MITPTSFEPV ELLYSKKKVN RTNWKKNVLK SNPEINNLCE RIYPINRAIQ
60 70 80 90 100
FGKQFHPGKA EVKQKLDKTA IIQLILQHLS TKGLKQTKQT LEKEARTTTP
110 120 130 140 150
IVEGLNESRL VTYIRNALKD TDRIYDLSME HTEYSKEERQ SKITEREELL
160 170 180 190 200
FQMDLLEDED EDDGVNIWDE PTENIITEKV HTTEYDINKS KENKDDQDDE
210 220 230 240 250
VVKFASLNKL VEHLTHDSKH DLQFLKTFLM TYQSFCTPEK LMSKLQQRYN
260 270 280 290 300
CPSGHDEMAT RNIQIRVINV LKGWVDNYYS DFDDKLIAML RTFIDQIQIK
310 320 330 340 350
FPAPASAVNK SLTKMVEKLS PVNDSKHIFN EKTPEPMVPK NIFSNNLSIY
360 370 380 390 400
DIDEEEIARQ LTLIEFEIYR NIKPPELLNQ SWNKTKLKSR APNVLKMIDR
410 420 430 440 450
FNSVSMWVAT MIIQTTKVKA RARMMTRFIK IADHLKNLNN YNSLMAIIAG
460 470 480 490 500
LNFSSVYRLK YTREELSAQT MRTYSDLEKI MNSEGSFKTY RTRLQNVPPM
510 520 530 540 550
LPYLGVHLTD LTFIDENPNN FVTDVGGKQV SLINFTKRTL VFKIISLIQE
560 570 580 590 600
TQVVPYNLQP VHQIQEFLLN IRSDLKAHTL DQYQQELYRE SLKREPKKAQ

RSDVL
Length:605
Mass (Da):70,793
Last modified:May 24, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB9ABCF37604278B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti17K → E in AAB09441 (PubMed:8793298).Curated1
Sequence conflicti17K → E in AAN46870 (PubMed:16086850).Curated1
Sequence conflicti20N → D in AAB09441 (PubMed:8793298).Curated1
Sequence conflicti20N → D in AAN46870 (PubMed:16086850).Curated1
Sequence conflicti23 – 26NWKK → EWRR in AAB09441 (PubMed:8793298).Curated4
Sequence conflicti23 – 26NWKK → EWRR in AAN46870 (PubMed:16086850).Curated4
Sequence conflicti51F → L in AAN46870 (PubMed:16086850).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U53884 mRNA Translation: AAB09441.1
AY160090 Genomic DNA Translation: AAN46870.1
AAFI02000064 Genomic DNA Translation: EAL65212.1

Genome annotation databases

Ensembl protists genome annotation project

More...
EnsemblProtistsi
EAL65212; EAL65212; DDB_G0284329

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ddi:DDB_G0284329

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53884 mRNA Translation: AAB09441.1
AY160090 Genomic DNA Translation: AAN46870.1
AAFI02000064 Genomic DNA Translation: EAL65212.1

3D structure databases

SMRiQ54PQ4
ModBaseiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191187

Proteomic databases

PaxDbiQ54PQ4
PRIDEiQ54PQ4

Genome annotation databases

EnsemblProtistsiEAL65212; EAL65212; DDB_G0284329
KEGGiddi:DDB_G0284329

Organism-specific databases

dictyBaseiDDB_G0284329, gefA

Phylogenomic databases

eggNOGiKOG3417, Eukaryota
HOGENOMiCLU_452309_0_0_1
InParanoidiQ54PQ4
OMAiITAICEP
PhylomeDBiQ54PQ4

Enzyme and pathway databases

ReactomeiR-DDI-1169092, Activation of RAS in B cells
R-DDI-354192, Integrin signaling
R-DDI-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-DDI-392517, Rap1 signalling
R-DDI-5673001, RAF/MAP kinase cascade

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q54PQ4

Family and domain databases

CDDicd00155, RasGEF, 1 hit
cd06224, REM, 1 hit
Gene3Di1.10.840.10, 1 hit
InterProiView protein in InterPro
IPR006594, LisH
IPR008937, Ras-like_GEF
IPR000651, Ras-like_Gua-exchang_fac_N
IPR023578, Ras_GEF_dom_sf
IPR001895, RASGEF_cat_dom
IPR036964, RASGEF_cat_dom_sf
PANTHERiPTHR23113, PTHR23113, 1 hit
PfamiView protein in Pfam
PF00617, RasGEF, 1 hit
PF00618, RasGEF_N, 1 hit
SMARTiView protein in SMART
SM00667, LisH, 1 hit
SM00147, RasGEF, 1 hit
SM00229, RasGEFN, 1 hit
SUPFAMiSSF48366, SSF48366, 1 hit
PROSITEiView protein in PROSITE
PS50896, LISH, 1 hit
PS50009, RASGEF_CAT, 1 hit
PS50212, RASGEF_NTER, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGEFA_DICDI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q54PQ4
Secondary accession number(s): Q23867, Q8IS22
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 24, 2005
Last modified: October 7, 2020
This is version 95 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
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