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UniProtKB - Q54NW7 (VAM7A_DICDI)
Protein
Vesicle-associated membrane protein 7A
Gene
vamp7A
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis.
By similarity1 PublicationGO - Biological processi
- endosome to lysosome transport Source: UniProtKB
- protein transport Source: UniProtKB-KW
- vesicle fusion Source: UniProtKB
- vesicle-mediated transport Source: UniProtKB
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-DDI-199992, trans-Golgi Network Vesicle Budding |
Names & Taxonomyi
Protein namesi | Recommended name: Vesicle-associated membrane protein 7A |
Gene namesi | ORF Names:DDB_G0284951 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0284951, vamp7A |
Subcellular locationi
Endosome
- Late endosome membrane 1 Publication; Single-pass type IV membrane protein 1 Publication
Golgi apparatus
- trans-Golgi network membrane Sequence analysisBy similarity; Single-pass type IV membrane protein Sequence analysisBy similarity
Endoplasmic reticulum
- Endoplasmic reticulum membrane Sequence analysisBy similarity; Single-pass type IV membrane protein Sequence analysisBy similarity
Lysosome
- Lysosome membrane 1 Publication; Single-pass type IV membrane protein 1 Publication
Other locations
- secretory vesicle membrane 1 Publication; Single-pass type IV membrane protein 1 Publication
- phagosome membrane Sequence analysisBy similarity; Single-pass type IV membrane protein Sequence analysisBy similarity
Note: During macropinocytosis highest levels are found in endosomes with lower levels observed in the lysosomes.Sequence analysisBy similarity1 Publication
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
Endosome
- endosome Source: dictyBase
- late endosome membrane Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Lysosome
- lysosomal membrane Source: UniProtKB
Vacuole
- lysosomal membrane Source: UniProtKB
Other locations
- endocytic vesicle membrane Source: dictyBase
- integral component of membrane Source: UniProtKB-KW
- phagocytic vesicle Source: dictyBase
- phagocytic vesicle membrane Source: UniProtKB-SubCell
- SNARE complex Source: UniProtKB
- transport vesicle membrane Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 189 | CytoplasmicSequence analysisBy similarityAdd BLAST | 189 | |
Transmembranei | 190 – 210 | Helical; Anchor for type IV membrane proteinAdd BLAST | 21 | |
Topological domaini | 211 – 216 | VesicularSequence analysisBy similarity | 6 |
Keywords - Cellular componenti
Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000320001 | 1 – 216 | Vesicle-associated membrane protein 7AAdd BLAST | 216 |
Proteomic databases
PaxDbi | Q54NW7 |
Interactioni
Subunit structurei
Component of the SNARE complex composed of syn7A, syn8A, vamp7A and vti1A.
1 PublicationBinary interactionsi
Q54NW7
With | #Exp. | IntAct |
---|---|---|
syn7A [Q54JY7] | 6 | EBI-1810311,EBI-1810238 |
Protein-protein interaction databases
IntActi | Q54NW7, 1 interactor |
STRINGi | 44689.DDB0231535 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 112 | LonginPROSITE-ProRule annotationAdd BLAST | 107 | |
Domaini | 126 – 186 | v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST | 61 |
Sequence similaritiesi
Belongs to the synaptobrevin family.Curated
Keywords - Domaini
Coiled coil, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0859, Eukaryota |
HOGENOMi | CLU_064620_1_1_1 |
InParanoidi | Q54NW7 |
OMAi | SKFFIHC |
PhylomeDBi | Q54NW7 |
Family and domain databases
CDDi | cd14824, Longin, 1 hit |
InterProi | View protein in InterPro IPR011012, Longin-like_dom_sf IPR010908, Longin_dom IPR001388, Synaptobrevin IPR042855, V_SNARE_CC |
Pfami | View protein in Pfam PF13774, Longin, 1 hit PF00957, Synaptobrevin, 1 hit |
PRINTSi | PR00219, SYNAPTOBREVN |
SMARTi | View protein in SMART SM01270, Longin, 1 hit |
SUPFAMi | SSF64356, SSF64356, 1 hit |
PROSITEi | View protein in PROSITE PS50859, LONGIN, 1 hit PS50892, V_SNARE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q54NW7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQTDILYAC VSYKGVCLVE HKIANGNFID LARRLITKIP PTSKKIYTSE
60 70 80 90 100
NHNFHYISEN DLAFLCLCHE KLGVQIPSEF LSDIRQQFIR SYGQSFSQNS
110 120 130 140 150
PTATYDPFIR VLEERMKYYS NPKSNKMNLV MDQVSEAKGA LTDAIEKTIH
160 170 180 190 200
RGEKIEIIVD KTERLQSESF VFKSNSVALK RKLWWQNKKL AIAIGLVVCI
210
LIAVITLALL KYFKVI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000073 Genomic DNA Translation: EAL64939.1 |
RefSeqi | XP_639950.1, XM_634858.1 |
Genome annotation databases
EnsemblProtistsi | EAL64939; EAL64939; DDB_G0284951 |
GeneIDi | 8624862 |
KEGGi | ddi:DDB_G0284951 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000073 Genomic DNA Translation: EAL64939.1 |
RefSeqi | XP_639950.1, XM_634858.1 |
3D structure databases
AlphaFoldDBi | Q54NW7 |
SMRi | Q54NW7 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q54NW7, 1 interactor |
STRINGi | 44689.DDB0231535 |
Proteomic databases
PaxDbi | Q54NW7 |
Genome annotation databases
EnsemblProtistsi | EAL64939; EAL64939; DDB_G0284951 |
GeneIDi | 8624862 |
KEGGi | ddi:DDB_G0284951 |
Organism-specific databases
dictyBasei | DDB_G0284951, vamp7A |
Phylogenomic databases
eggNOGi | KOG0859, Eukaryota |
HOGENOMi | CLU_064620_1_1_1 |
InParanoidi | Q54NW7 |
OMAi | SKFFIHC |
PhylomeDBi | Q54NW7 |
Enzyme and pathway databases
Reactomei | R-DDI-199992, trans-Golgi Network Vesicle Budding |
Miscellaneous databases
PROi | PR:Q54NW7 |
Family and domain databases
CDDi | cd14824, Longin, 1 hit |
InterProi | View protein in InterPro IPR011012, Longin-like_dom_sf IPR010908, Longin_dom IPR001388, Synaptobrevin IPR042855, V_SNARE_CC |
Pfami | View protein in Pfam PF13774, Longin, 1 hit PF00957, Synaptobrevin, 1 hit |
PRINTSi | PR00219, SYNAPTOBREVN |
SMARTi | View protein in SMART SM01270, Longin, 1 hit |
SUPFAMi | SSF64356, SSF64356, 1 hit |
PROSITEi | View protein in PROSITE PS50859, LONGIN, 1 hit PS50892, V_SNARE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VAM7A_DICDI | |
Accessioni | Q54NW7Primary (citable) accession number: Q54NW7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | May 24, 2005 | |
Last modified: | May 25, 2022 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - SIMILARITY comments
Index of protein domains and families