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UniProtKB - Q54GJ7 (HOT_DICDI)
Protein
Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
Gene
adhfe1
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).
By similarityCatalytic activityi
GO - Molecular functioni
- alcohol dehydrogenase (NAD+) activity Source: GO_Central
- hydroxyacid-oxoacid transhydrogenase activity Source: UniProtKB
- metal ion binding Source: InterPro
GO - Biological processi
- glutamate catabolic process via 2-oxoglutarate Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Enzyme and pathway databases
Reactomei | R-DDI-880009, Interconversion of 2-oxoglutarate and 2-hydroxyglutarate |
Names & Taxonomyi
Protein namesi | Recommended name: Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial (EC:1.1.99.24)Short name: HOT |
Gene namesi | Name:adhfe1 ORF Names:DDB_G0290111 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0290111, adhfe1 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrion Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000323002 | ? – 547 | Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial |
Proteomic databases
PaxDbi | Q54GJ7 |
Family & Domainsi
Sequence similaritiesi
Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3857, Eukaryota |
HOGENOMi | CLU_007207_0_7_1 |
InParanoidi | Q54GJ7 |
OMAi | IRFGPGC |
PhylomeDBi | Q54GJ7 |
Family and domain databases
CDDi | cd08190, HOT, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR042157, HOT |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q54GJ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTIIITKIAD KIIKSNRVKN TLQDIHIHSS NCGCHGSFKS NSVMNQTFSS
60 70 80 90 100
FSSLTTEKNE QQQQQLFKQF SNSKPIPMGR KVELDTSKVI DSVYFGNTEN
110 120 130 140 150
NNYRTNKGNT DYAFEASANN IRFGSGVTYE VGYDLLDMNC RNVIVFTDNN
160 170 180 190 200
LLKLVDTDKE SAVAKCLYSL EKCGIKYTIY SDVSIEPTDT SFKDAISVMG
210 220 230 240 250
KGRYDGVVAV GGGSVMDTAK AANLYNSYPP ADNDFLAYIN PPIGKGLLVP
260 270 280 290 300
GPLKRPLIAI PTTCGTASET TGVCILDIKL GDGLSAKTGI ASRHLKPILG
310 320 330 340 350
LVDPDNLLTL PSNVAISSGF DQLCHALESF TAIPFNQRSP RPLAPNQRPS
360 370 380 390 400
YQGANPVSDV WSLRSLEMLC KNIHRFVLNP NDDYARSQMM LAASYAGLGF
410 420 430 440 450
GNSGVHACHG MSYSISSMVK DYKPEGYYGL KKNLIPHGQS VILSAPAVFK
460 470 480 490 500
FTAPSNPERH LLLAKIMGAD ISNASESDAG VLLSNQIVKL MKLLNVPNGL
510 520 530 540
QALGYKESDI DSLVKGTLPQ HRVTKLMPKQ ATYDDLYKLF KDSMTIY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000153 Genomic DNA Translation: EAL62385.1 |
RefSeqi | XP_635889.1, XM_630797.1 |
Genome annotation databases
EnsemblProtistsi | EAL62385; EAL62385; DDB_G0290111 |
GeneIDi | 8627488 |
KEGGi | ddi:DDB_G0290111 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000153 Genomic DNA Translation: EAL62385.1 |
RefSeqi | XP_635889.1, XM_630797.1 |
3D structure databases
AlphaFoldDBi | Q54GJ7 |
SMRi | Q54GJ7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 44689.DDB0252839 |
Proteomic databases
PaxDbi | Q54GJ7 |
Genome annotation databases
EnsemblProtistsi | EAL62385; EAL62385; DDB_G0290111 |
GeneIDi | 8627488 |
KEGGi | ddi:DDB_G0290111 |
Organism-specific databases
dictyBasei | DDB_G0290111, adhfe1 |
Phylogenomic databases
eggNOGi | KOG3857, Eukaryota |
HOGENOMi | CLU_007207_0_7_1 |
InParanoidi | Q54GJ7 |
OMAi | IRFGPGC |
PhylomeDBi | Q54GJ7 |
Enzyme and pathway databases
Reactomei | R-DDI-880009, Interconversion of 2-oxoglutarate and 2-hydroxyglutarate |
Miscellaneous databases
PROi | PR:Q54GJ7 |
Family and domain databases
CDDi | cd08190, HOT, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR042157, HOT |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HOT_DICDI | |
Accessioni | Q54GJ7Primary (citable) accession number: Q54GJ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | May 24, 2005 | |
Last modified: | May 25, 2022 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - SIMILARITY comments
Index of protein domains and families