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UniProtKB - Q54E83 (GSHB_DICDI)
Protein
Glutathione synthetase
Gene
gshB
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
Catalytic activityi
- EC:6.3.2.3
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
: glutathione biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate. This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 117 | SubstrateBy similarity | 1 | |
Metal bindingi | 137 | MagnesiumBy similarity | 1 | |
Binding sitei | 137 | ATPBy similarity | 1 | |
Metal bindingi | 139 | MagnesiumBy similarity | 1 | |
Binding sitei | 217 | SubstrateBy similarity | 1 | |
Binding sitei | 308 | ATPBy similarity | 1 | |
Metal bindingi | 371 | MagnesiumBy similarity | 1 | |
Binding sitei | 378 | ATPBy similarity | 1 | |
Binding sitei | 426 | ATPBy similarity | 1 | |
Binding sitei | 452 | SubstrateBy similarity | 1 | |
Binding sitei | 454 | ATPBy similarity | 1 | |
Binding sitei | 460 | ATP; via carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 367 – 376 | ATPBy similarity | 10 | |
Nucleotide bindingi | 400 – 403 | ATPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- glutathione binding Source: UniProtKB
- glutathione synthase activity Source: dictyBase
- magnesium ion binding Source: UniProtKB
GO - Biological processi
- glutathione biosynthetic process Source: dictyBase
Keywordsi
Molecular function | Ligase |
Biological process | Glutathione biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DDI-174403, Glutathione synthesis and recycling |
UniPathwayi | UPA00142;UER00210 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione synthetase (EC:6.3.2.3)Short name: GSH synthetase Short name: GSH-S Alternative name(s): Glutathione synthase |
Gene namesi | Name:gshB ORF Names:DDB_G0291756 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0291756, gshB |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000312710 | 1 – 476 | Glutathione synthetaseAdd BLAST | 476 |
Proteomic databases
PaxDbi | Q54E83 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 44689.DDB0231404 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 141 – 144 | Substrate bindingBy similarity | 4 | |
Regioni | 211 – 213 | Substrate bindingBy similarity | 3 | |
Regioni | 267 – 270 | Substrate bindingBy similarity | 4 | |
Regioni | 463 – 464 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the eukaryotic GSH synthase family.Curated
Phylogenomic databases
eggNOGi | KOG0021, Eukaryota |
HOGENOMi | CLU_025152_2_1_1 |
InParanoidi | Q54E83 |
OMAi | CPTVLTQ |
PhylomeDBi | Q54E83 |
Family and domain databases
Gene3Di | 1.10.1080.10, 1 hit 3.30.1490.50, 1 hit 3.30.1490.80, 1 hit 3.40.50.1760, 1 hit |
InterProi | View protein in InterPro IPR005615, Glutathione_synthase IPR014042, Glutathione_synthase_a-hlx IPR014709, Glutathione_synthase_C_euk IPR014049, Glutathione_synthase_N_euk IPR037013, GSH-S_sub-bd_sf IPR004887, GSH_synth_subst-bd IPR016185, PreATP-grasp_dom_sf |
PANTHERi | PTHR11130, PTHR11130, 1 hit |
Pfami | View protein in Pfam PF03917, GSH_synth_ATP, 1 hit PF03199, GSH_synthase, 1 hit |
PIRSFi | PIRSF001558, GSHase, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01986, glut_syn_euk, 1 hit |
i Sequence
Sequence statusi: Complete.
Q54E83-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEEKLNDLKE QGIDWAFANG LIMIKKPTEE EAKNNVVNVT HVPFSLYPSK
60 70 80 90 100
MNKKLFNEAC KLAEDYNLLV HNISKDYDFL QNTLKDVFDD FTQMLLNIQR
110 120 130 140 150
KVVKEGIKQK ISLGIFRSDY MFHNKIEGEE ERIYQVELNT ISSSLAVVSN
160 170 180 190 200
RVFNLHKYLI GRNDLNDNGY DLLNHPTNQS DKEISDSIAL AHKLYNKEKS
210 220 230 240 250
SVVLMIIQEG ERNIYDQKGL EFQLWSNHSI KLIRRTMKEI NQCAKLDEEN
260 270 280 290 300
GSVLIVDGME ISVAYYRAGY TPNDYTSSGG DEWKARLLIE RSLAIKCPTI
310 320 330 340 350
AHHLVGVKKI QQVLAQPGVL EKFINNDKES LQRVKRSFTG LYSLSKEDID
360 370 380 390 400
MSVVKEAIES PQNYVMKPQR EGGGNNIYND QVAIALKSMS SEELSSYILM
410 420 430 440 450
DKIMSKSFKT HVVRDRQLLE IEGLYELGIY SVFISNGDDD IVLNKQAGIL
460 470
LRTKTANSDE VGVAAGFGLL DSPILE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000182 Genomic DNA Translation: EAL61574.1 |
RefSeqi | XP_629979.1, XM_629977.1 |
Genome annotation databases
EnsemblProtistsi | EAL61574; EAL61574; DDB_G0291756 |
GeneIDi | 8628309 |
KEGGi | ddi:DDB_G0291756 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000182 Genomic DNA Translation: EAL61574.1 |
RefSeqi | XP_629979.1, XM_629977.1 |
3D structure databases
SMRi | Q54E83 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 44689.DDB0231404 |
Proteomic databases
PaxDbi | Q54E83 |
Genome annotation databases
EnsemblProtistsi | EAL61574; EAL61574; DDB_G0291756 |
GeneIDi | 8628309 |
KEGGi | ddi:DDB_G0291756 |
Organism-specific databases
dictyBasei | DDB_G0291756, gshB |
Phylogenomic databases
eggNOGi | KOG0021, Eukaryota |
HOGENOMi | CLU_025152_2_1_1 |
InParanoidi | Q54E83 |
OMAi | CPTVLTQ |
PhylomeDBi | Q54E83 |
Enzyme and pathway databases
UniPathwayi | UPA00142;UER00210 |
Reactomei | R-DDI-174403, Glutathione synthesis and recycling |
Miscellaneous databases
PROi | PR:Q54E83 |
Family and domain databases
Gene3Di | 1.10.1080.10, 1 hit 3.30.1490.50, 1 hit 3.30.1490.80, 1 hit 3.40.50.1760, 1 hit |
InterProi | View protein in InterPro IPR005615, Glutathione_synthase IPR014042, Glutathione_synthase_a-hlx IPR014709, Glutathione_synthase_C_euk IPR014049, Glutathione_synthase_N_euk IPR037013, GSH-S_sub-bd_sf IPR004887, GSH_synth_subst-bd IPR016185, PreATP-grasp_dom_sf |
PANTHERi | PTHR11130, PTHR11130, 1 hit |
Pfami | View protein in Pfam PF03917, GSH_synth_ATP, 1 hit PF03199, GSH_synthase, 1 hit |
PIRSFi | PIRSF001558, GSHase, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01986, glut_syn_euk, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GSHB_DICDI | |
Accessioni | Q54E83Primary (citable) accession number: Q54E83 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 4, 2007 |
Last sequence update: | May 24, 2005 | |
Last modified: | February 23, 2022 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families